Lipovitellins and phosvitins of the fertilized eggs during embryo growth in the oviparous lizard Podarcis sicula
Identifieur interne : 001399 ( Main/Merge ); précédent : 001398; suivant : 001400Lipovitellins and phosvitins of the fertilized eggs during embryo growth in the oviparous lizard Podarcis sicula
Auteurs : Maurizio Romano [Italie] ; Patrizia Rosanova [Italie] ; Chiara Anteo [Italie] ; Ermelinda Limatola [Italie]Source :
- Molecular Reproduction and Development [ 1040-452X ] ; 2002-11.
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Abstract
In the lizard Podarcis sicula, the major vitellogenin (VTG)‐derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were isolated by precipitation in saturated (NH4)2SO4, separated on SDS–PAGE and detected by Western blotting with homologous polyclonal anti/VTG antibody. Two lipovitellins of 110 and 116 kDa were always present in the yolk of laid eggs after 1, 10, 18, and 44 days from oviposition. Both these proteins were glycosylated and were recognized by the anti/VTG antibody; their N‐terminal sequences were analyzed. Four phosvitins were detected in freshly laid eggs, but their number decreased during incubation, and after 44 days only a single protein of ≅ 6.5 kDa was present. The results indicated that, in this lizard, during embryonic development, lipovitellins remain unchanged, whereas the phosphorylated components of yolk undergo continuous degradation. Mol. Reprod. Dev. 63: 341–348, 2002. © 2002 Wiley‐Liss, Inc.
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DOI: 10.1002/mrd.90018
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Dev.</title><idno type="ISSN">1040-452X</idno><idno type="eISSN">1098-2795</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="2002-11">2002-11</date><biblScope unit="volume">63</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="341">341</biblScope><biblScope unit="page" to="348">348</biblScope></imprint><idno type="ISSN">1040-452X</idno></series><idno type="istex">4F803E60EA21A29F87B93E36DED42422E7899F63</idno><idno type="DOI">10.1002/mrd.90018</idno><idno type="ArticleID">MRD90018</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1040-452X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>albumen</term><term>embryonic development</term><term>reptiles</term><term>vitellogenesis</term><term>yolk proteins</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the lizard Podarcis sicula, the major vitellogenin (VTG)‐derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were isolated by precipitation in saturated (NH4)2SO4, separated on SDS–PAGE and detected by Western blotting with homologous polyclonal anti/VTG antibody. Two lipovitellins of 110 and 116 kDa were always present in the yolk of laid eggs after 1, 10, 18, and 44 days from oviposition. Both these proteins were glycosylated and were recognized by the anti/VTG antibody; their N‐terminal sequences were analyzed. Four phosvitins were detected in freshly laid eggs, but their number decreased during incubation, and after 44 days only a single protein of ≅ 6.5 kDa was present. The results indicated that, in this lizard, during embryonic development, lipovitellins remain unchanged, whereas the phosphorylated components of yolk undergo continuous degradation. Mol. Reprod. Dev. 63: 341–348, 2002. © 2002 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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