Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation.
Identifieur interne : 001354 ( Main/Merge ); précédent : 001353; suivant : 001355Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation.
Auteurs : Molly A H. Webb [États-Unis] ; Grant W. Feist ; John M. Trant ; Joel P. Van Eenennaam ; Martin S. Fitzpatrick ; Carl B. Schreck ; Serge I. DoroshovSource :
- General and comparative endocrinology [ 0016-6480 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Steroids.
- blood : Ovulation.
- growth & development : Oocytes.
- metabolism : Fishes, Oocytes, Ovarian Follicle, Ovary.
- Animals, Chromatography, High Pressure Liquid, Female, In Vitro Techniques, Ovulation Induction.
Abstract
Ovarian follicles and plasma were collected from two female white sturgeon, Acipenser transmontanus, injected with sturgeon pituitary homogenate followed 12h later with GnRHa to induce ovulation. The oocytes of one female underwent germinal vesicle breakdown (GVBD) but ovulation did not occur in response to hormonal stimulation (Female 1), while the oocytes of the other female underwent GVBD and ovulation (Female 2). Follicles collected 12h after the first injection to induce ovulation were incubated with radioinert pregnenolone (P5) or tritiated-P5 ([3H]P5) plus radioinert P5. Steroids were extracted from media and intact follicles, and the extracts were fractionated by high performance liquid chromatography (HPLC). Fractions from the incubation with radioinert precursor were used in a bioassay to determine the potency of the steroid products to induce GVBD. Plasma levels of testosterone (T), estradiol, and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) were measured by radioimmunoassay during induced ovulation, and plasma collected at the time of ovulation (actual or expected) was analyzed by HPLC. A peak in plasma 17,20beta-P was detected at the time of the second injection to induce ovulation in Female 2 (the time at which follicles were collected for incubation with [3H]P5). The HPLC analysis revealed several progestins in the plasma of Female 2 at ovulation that were not present in Female 1 at the time of expected ovulation. A variety of C19 and C21 steroids were produced in vitro by ovarian follicles from both females. The "suggestive" identities of the major metabolites were 11-deoxycortisol, androstenedione, 17-hydroxyprogesterone (17OHP), and 17,20beta-P in Female 1 and cortisol, 17,20beta, 21-trihydroxyprogesterone (20beta-S), 11-deoxycortisol, T, 17OHP, and 17,20beta-P in Female 2. Several of the steroids were active in a GVBD bioassay, but the fractions that contained the steroid coeluting the authentic 11-deoxycortisol on the HPLC and 17,20beta-P (positively identified by gas chromatography-mass spectrometry) were found to be the most potent. The results from this study combined with the results of Webb et al. (2001b) suggest the potential roles of 11-deoxycortisol, 17,20beta-P, 20beta-S, and P4 as maturation-inducing steroids in sturgeon.
PubMed: 12409093
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000599
- to stream PubMed, to step Curation: 000599
- to stream PubMed, to step Checkpoint: 000599
- to stream Ncbi, to step Merge: 000075
- to stream Ncbi, to step Curation: 000075
- to stream Ncbi, to step Checkpoint: 000075
Links to Exploration step
pubmed:12409093Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation.</title>
<author><name sortKey="Webb, Molly A H" sort="Webb, Molly A H" uniqKey="Webb M" first="Molly A H" last="Webb">Molly A H. Webb</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Animal Science, One Shields Avenue, University of California, Davis, CA 95616, USA. webbm@onid.orst.edu</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Animal Science, One Shields Avenue, University of California, Davis, CA 95616</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feist, Grant W" sort="Feist, Grant W" uniqKey="Feist G" first="Grant W" last="Feist">Grant W. Feist</name>
</author>
<author><name sortKey="Trant, John M" sort="Trant, John M" uniqKey="Trant J" first="John M" last="Trant">John M. Trant</name>
</author>
<author><name sortKey="Van Eenennaam, Joel P" sort="Van Eenennaam, Joel P" uniqKey="Van Eenennaam J" first="Joel P" last="Van Eenennaam">Joel P. Van Eenennaam</name>
</author>
<author><name sortKey="Fitzpatrick, Martin S" sort="Fitzpatrick, Martin S" uniqKey="Fitzpatrick M" first="Martin S" last="Fitzpatrick">Martin S. Fitzpatrick</name>
</author>
<author><name sortKey="Schreck, Carl B" sort="Schreck, Carl B" uniqKey="Schreck C" first="Carl B" last="Schreck">Carl B. Schreck</name>
</author>
<author><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I" last="Doroshov">Serge I. Doroshov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2002">2002</date>
<idno type="RBID">pubmed:12409093</idno>
<idno type="pmid">12409093</idno>
<idno type="wicri:Area/PubMed/Corpus">000599</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000599</idno>
<idno type="wicri:Area/PubMed/Curation">000599</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000599</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000599</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000599</idno>
<idno type="wicri:Area/Ncbi/Merge">000075</idno>
<idno type="wicri:Area/Ncbi/Curation">000075</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000075</idno>
<idno type="wicri:doubleKey">0016-6480:2002:Webb M:ovarian:steroidogenesis:in</idno>
<idno type="wicri:Area/Main/Merge">001354</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation.</title>
<author><name sortKey="Webb, Molly A H" sort="Webb, Molly A H" uniqKey="Webb M" first="Molly A H" last="Webb">Molly A H. Webb</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Animal Science, One Shields Avenue, University of California, Davis, CA 95616, USA. webbm@onid.orst.edu</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Animal Science, One Shields Avenue, University of California, Davis, CA 95616</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feist, Grant W" sort="Feist, Grant W" uniqKey="Feist G" first="Grant W" last="Feist">Grant W. Feist</name>
</author>
<author><name sortKey="Trant, John M" sort="Trant, John M" uniqKey="Trant J" first="John M" last="Trant">John M. Trant</name>
</author>
<author><name sortKey="Van Eenennaam, Joel P" sort="Van Eenennaam, Joel P" uniqKey="Van Eenennaam J" first="Joel P" last="Van Eenennaam">Joel P. Van Eenennaam</name>
</author>
<author><name sortKey="Fitzpatrick, Martin S" sort="Fitzpatrick, Martin S" uniqKey="Fitzpatrick M" first="Martin S" last="Fitzpatrick">Martin S. Fitzpatrick</name>
</author>
<author><name sortKey="Schreck, Carl B" sort="Schreck, Carl B" uniqKey="Schreck C" first="Carl B" last="Schreck">Carl B. Schreck</name>
</author>
<author><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I" last="Doroshov">Serge I. Doroshov</name>
</author>
</analytic>
<series><title level="j">General and comparative endocrinology</title>
<idno type="ISSN">0016-6480</idno>
<imprint><date when="2002" type="published">2002</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Female</term>
<term>Fishes (metabolism)</term>
<term>In Vitro Techniques</term>
<term>Oocytes (growth & development)</term>
<term>Oocytes (metabolism)</term>
<term>Ovarian Follicle (metabolism)</term>
<term>Ovary (metabolism)</term>
<term>Ovulation (blood)</term>
<term>Ovulation Induction</term>
<term>Steroids (blood)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Steroids</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Ovulation</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Oocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term>
<term>Oocytes</term>
<term>Ovarian Follicle</term>
<term>Ovary</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Female</term>
<term>In Vitro Techniques</term>
<term>Ovulation Induction</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Ovarian follicles and plasma were collected from two female white sturgeon, Acipenser transmontanus, injected with sturgeon pituitary homogenate followed 12h later with GnRHa to induce ovulation. The oocytes of one female underwent germinal vesicle breakdown (GVBD) but ovulation did not occur in response to hormonal stimulation (Female 1), while the oocytes of the other female underwent GVBD and ovulation (Female 2). Follicles collected 12h after the first injection to induce ovulation were incubated with radioinert pregnenolone (P5) or tritiated-P5 ([3H]P5) plus radioinert P5. Steroids were extracted from media and intact follicles, and the extracts were fractionated by high performance liquid chromatography (HPLC). Fractions from the incubation with radioinert precursor were used in a bioassay to determine the potency of the steroid products to induce GVBD. Plasma levels of testosterone (T), estradiol, and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) were measured by radioimmunoassay during induced ovulation, and plasma collected at the time of ovulation (actual or expected) was analyzed by HPLC. A peak in plasma 17,20beta-P was detected at the time of the second injection to induce ovulation in Female 2 (the time at which follicles were collected for incubation with [3H]P5). The HPLC analysis revealed several progestins in the plasma of Female 2 at ovulation that were not present in Female 1 at the time of expected ovulation. A variety of C19 and C21 steroids were produced in vitro by ovarian follicles from both females. The "suggestive" identities of the major metabolites were 11-deoxycortisol, androstenedione, 17-hydroxyprogesterone (17OHP), and 17,20beta-P in Female 1 and cortisol, 17,20beta, 21-trihydroxyprogesterone (20beta-S), 11-deoxycortisol, T, 17OHP, and 17,20beta-P in Female 2. Several of the steroids were active in a GVBD bioassay, but the fractions that contained the steroid coeluting the authentic 11-deoxycortisol on the HPLC and 17,20beta-P (positively identified by gas chromatography-mass spectrometry) were found to be the most potent. The results from this study combined with the results of Webb et al. (2001b) suggest the potential roles of 11-deoxycortisol, 17,20beta-P, 20beta-S, and P4 as maturation-inducing steroids in sturgeon.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001354 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001354 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:12409093 |texte= Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:12409093" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |