Screening for oestrogenic activity of plant and food extracts using in vitro trout hepatocyte cultures
Identifieur interne : 001091 ( Main/Merge ); précédent : 001090; suivant : 001092Screening for oestrogenic activity of plant and food extracts using in vitro trout hepatocyte cultures
Auteurs : C. Bennetau-Pelissero [France] ; K. Gontier Latonnelle [France] ; V. Lamothe [France] ; S. Shinkaruk-Poix [France] ; S. J. Kaushik [France]Source :
- Phytochemical Analysis [ 0958-0344 ] ; 2004-01.
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Abstract
The use of in vitro trout hepatocyte cultures is shown to provide a simple and effective way to screen plant and food products for oestrogenic activity. The relative oestrogenic activities of 0.1 g each of extracts of phytosterol, soy isoflavone, red clover, kudzu and soybean extracts were determined using this assay and found to be equivalent to 212, 1, 3.2, 132 and 1025 nm of 17β‐estradiol, respectively. Controls were performed on soybean and kudzu extracts using specific ELISAs for isoflavones and these confirmed the validity of the cell culture assay. The method described offers an advantage over current methods in that it can detect increased oestrogenic activity that may occur as a result of metabolic activation of pre‐ or pro‐oestrogens liver cells. Copyright © 2004 John Wiley & Sons, Ltd.
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DOI: 10.1002/pca.741
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The relative oestrogenic activities of 0.1 g each of extracts of phytosterol, soy isoflavone, red clover, kudzu and soybean extracts were determined using this assay and found to be equivalent to 212, 1, 3.2, 132 and 1025 nm of 17β‐estradiol, respectively. Controls were performed on soybean and kudzu extracts using specific ELISAs for isoflavones and these confirmed the validity of the cell culture assay. The method described offers an advantage over current methods in that it can detect increased oestrogenic activity that may occur as a result of metabolic activation of pre‐ or pro‐oestrogens liver cells. Copyright © 2004 John Wiley & Sons, Ltd.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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