The influence of oocyte maturational stage on hatching and triploidy rates in hybrid (bester) sturgeon, Huso huso × Acipneser ruthenus
Identifieur interne : 001017 ( Main/Merge ); précédent : 001016; suivant : 001018The influence of oocyte maturational stage on hatching and triploidy rates in hybrid (bester) sturgeon, Huso huso × Acipneser ruthenus
Auteurs : Naotaka Omoto [Japon] ; Mamoru Maebayashi [Japon] ; Shinji Adachi [Japon] ; Katsutoshi Arai [Japon] ; Kohei Yamauchi [Japon]Source :
- Aquaculture : (Amsterdam) [ 0044-8486 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Aquiculture, Milieu marin.
English descriptors
- KwdEn :
Abstract
We have investigated the relationship between maturational competence and hatching rate of eggs ovulated following injection of lutenizing hormone releasing hormone ethylamide (LH-RHa) in the cultured hybrid (bester) sturgeon (Huso huso female×Acipenser ruthenus male). Among 10 steroids tested (including progesterone) in a previous study, 17α-hydroxyprogesterone (17αOHP) was the most potent inducer of final maturation in the bester, with results similar to 17a,20p,21-trihydroxy-4-pregnen-3-one and 17α,20β-dihydroxy-4-pregnen-3-one. We therefore used 17αOHP to estimate maturational competence in the present study. In vitro oocyte maturation response to 17αOHP at a concentration of 0.1 μg/ml was correlated with ovulatory response to hormone treatment and quality of ovulated eggs. Two females exhibited higher in vitro oocyte maturation rate (100% germinal vesicle breakdown), ovulated sooner after hormone injection, and also had a higher hatching rate (77, 92%). In the F2 bester broodstock with a lower hatching rate (10-21%), a higher incidence (15-64%) of spontaneous triploidy was found. These results indicate that maturational competence can be estimated by in vitro oocyte maturation response to 17αOHP at 0.1 μg/ml and that this is a useful technique for selecting the proper female for breeding. Ovulatory success, hatching rate and occurrence of triploidy are thus all predictable in the cultured bester.
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067-0033</wicri:noRegion></affiliation></author><author><name sortKey="Maebayashi, Mamoru" sort="Maebayashi, Mamoru" uniqKey="Maebayashi M" first="Mamoru" last="Maebayashi">Mamoru Maebayashi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Research and Development, The Hokkaido Electric Power Co., Inc</s1><s2>Tsuishikari Ebetsu Hokkaido 067-0033</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Tsuishikari Ebetsu Hokkaido 067-0033</wicri:noRegion></affiliation></author><author><name sortKey="Adachi, Shinji" sort="Adachi, Shinji" uniqKey="Adachi S" first="Shinji" last="Adachi">Shinji Adachi</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University</s1><s2>Hakodate, Hokkaido 041-8611</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hakodate, Hokkaido 041-8611</wicri:noRegion></affiliation></author><author><name sortKey="Arai, Katsutoshi" sort="Arai, Katsutoshi" uniqKey="Arai K" first="Katsutoshi" last="Arai">Katsutoshi Arai</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University</s1><s2>Hakodate, Hokkaido 041-8611</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hakodate, Hokkaido 041-8611</wicri:noRegion></affiliation></author><author><name sortKey="Yamauchi, Kohei" sort="Yamauchi, Kohei" uniqKey="Yamauchi K" first="Kohei" last="Yamauchi">Kohei Yamauchi</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University</s1><s2>Hakodate, Hokkaido 041-8611</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hakodate, Hokkaido 041-8611</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Aquaculture : (Amsterdam)</title><title level="j" type="abbreviated">Aquaculture : (Amst.)</title><idno type="ISSN">0044-8486</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Aquaculture : (Amsterdam)</title><title level="j" type="abbreviated">Aquaculture : (Amst.)</title><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser sturio</term><term>Aquaculture</term><term>Egg</term><term>Hatching</term><term>Huso huso</term><term>Hybrid</term><term>In vitro</term><term>Marine environment</term><term>Oocyte</term><term>Oocyte maturation</term><term>Spawning</term><term>Triploidy</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Ovocyte</term><term>Eclosion</term><term>Triploïdie</term><term>Hybride</term><term>Frai</term><term>Oeuf</term><term>In vitro</term><term>Maturation oeuf</term><term>Aquiculture</term><term>Milieu marin</term><term>Acipenser sturio</term><term>Huso huso</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Aquiculture</term><term>Milieu marin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated the relationship between maturational competence and hatching rate of eggs ovulated following injection of lutenizing hormone releasing hormone ethylamide (LH-RHa) in the cultured hybrid (bester) sturgeon (Huso huso female×Acipenser ruthenus male). Among 10 steroids tested (including progesterone) in a previous study, 17α-hydroxyprogesterone (17αOHP) was the most potent inducer of final maturation in the bester, with results similar to 17a,20p,21-trihydroxy-4-pregnen-3-one and 17α,20β-dihydroxy-4-pregnen-3-one. We therefore used 17αOHP to estimate maturational competence in the present study. In vitro oocyte maturation response to 17αOHP at a concentration of 0.1 μg/ml was correlated with ovulatory response to hormone treatment and quality of ovulated eggs. Two females exhibited higher in vitro oocyte maturation rate (100% germinal vesicle breakdown), ovulated sooner after hormone injection, and also had a higher hatching rate (77, 92%). In the F<sub>2</sub> bester broodstock with a lower hatching rate (10-21%), a higher incidence (15-64%) of spontaneous triploidy was found. These results indicate that maturational competence can be estimated by in vitro oocyte maturation response to 17αOHP at 0.1 μg/ml and that this is a useful technique for selecting the proper female for breeding. Ovulatory success, hatching rate and occurrence of triploidy are thus all predictable in the cultured bester.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Omoto, Naotaka" sort="Omoto, Naotaka" uniqKey="Omoto N" first="Naotaka" last="Omoto">Naotaka Omoto</name></noRegion><name sortKey="Adachi, Shinji" sort="Adachi, Shinji" uniqKey="Adachi S" first="Shinji" last="Adachi">Shinji Adachi</name><name sortKey="Arai, Katsutoshi" sort="Arai, Katsutoshi" uniqKey="Arai K" first="Katsutoshi" last="Arai">Katsutoshi Arai</name><name sortKey="Maebayashi, Mamoru" sort="Maebayashi, Mamoru" uniqKey="Maebayashi M" first="Mamoru" last="Maebayashi">Mamoru Maebayashi</name><name sortKey="Yamauchi, Kohei" sort="Yamauchi, Kohei" uniqKey="Yamauchi K" first="Kohei" last="Yamauchi">Kohei Yamauchi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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