Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods.
Identifieur interne : 000E21 ( Main/Merge ); précédent : 000E20; suivant : 000E22Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods.
Auteurs : M. Pate [Slovénie] ; V. Jencic ; M. Zolnir-Dovc ; M. OcepekSource :
- Diseases of aquatic organisms [ 0177-5103 ] ; 2005.
Descripteurs français
- Wicri :
- geographic : Slovénie.
English descriptors
- KwdEn :
- Animals, Aquaculture, Colony Count, Microbial, Fish Diseases (epidemiology), Fish Diseases (microbiology), Fishes, Genotype, Mycobacterium (genetics), Mycobacterium (isolation & purification), Mycobacterium Infections (epidemiology), Mycobacterium Infections (veterinary), Polymorphism, Restriction Fragment Length, Restriction Mapping, Slovenia (epidemiology), Species Specificity.
- MESH :
- geographic , epidemiology : Slovenia.
- epidemiology : Fish Diseases, Mycobacterium Infections.
- genetics : Mycobacterium.
- isolation & purification : Mycobacterium.
- microbiology : Fish Diseases.
- veterinary : Mycobacterium Infections.
- Animals, Aquaculture, Colony Count, Microbial, Fishes, Genotype, Polymorphism, Restriction Fragment Length, Restriction Mapping, Species Specificity.
Abstract
Thirty-five aquarium fish were investigated for the presence of mycobacteria by culture and molecular methods. The following species were examined: goldfish Carassius auratus auratus, guppy Poecilia reticulata, 4 three-spot gourami Trichogaster trichopterus, dwarf gourami Colisa lalia, Siamese fighting fish Betta splendens, freshwater angelfish Pterophyllum scalare, African cichlid fish Cichlidae spp., cichlid fish Microgeophagus altispinosus, cichlid fish Pseudotropheus lombardoi, blue streak hap Labidochromis caeruleus, sterlet Acipenser ruthenus, southern platyfish Xiphophorus maculatus, and catfish Corydoras spp. Isolates of mycobacteria were obtained in 29 cases (82.9%). Two specimens were positive using Ziehl-Neelsen (ZN) staining, but the cultivation failed. Four specimens were both ZN- and culture-negative. On the basis of GenoType Mycobacterium assay (Hain Life-science) and restriction enzyme analysis of the amplified products (PCR-RFLP), 23 isolates (79.3%) were identified: 7 as Mycobacterium fortuitum, 6 as M. gordonae, 6 as M. marinum, 3 as M. chelonae, and 1 as M. peregrinum. Five isolates remained unidentified (Mycobacterium spp.). One case probably represented a mixed infection (M. marinum/M. fortuitum). Since M. marinum infections are also detected in humans, the significance of mycobacteria in aquarium fish should not be overlooked.
DOI: 10.3354/dao064029
PubMed: 15900685
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pubmed:15900685Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods.</title><author><name sortKey="Pate, M" sort="Pate, M" uniqKey="Pate M" first="M" last="Pate">M. Pate</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Faculty Ljubljana, University of Ljubljana, Gerbiceva 60, 1115 Ljubljana, Slovenia.</nlm:affiliation><country xml:lang="fr">Slovénie</country><wicri:regionArea>Veterinary Faculty Ljubljana, University of Ljubljana, Gerbiceva 60, 1115 Ljubljana</wicri:regionArea><wicri:noRegion>1115 Ljubljana</wicri:noRegion></affiliation></author><author><name sortKey="Jencic, V" sort="Jencic, V" uniqKey="Jencic V" first="V" last="Jencic">V. Jencic</name></author><author><name sortKey="Zolnir Dovc, M" sort="Zolnir Dovc, M" uniqKey="Zolnir Dovc M" first="M" last="Zolnir-Dovc">M. Zolnir-Dovc</name></author><author><name sortKey="Ocepek, M" sort="Ocepek, M" uniqKey="Ocepek M" first="M" last="Ocepek">M. Ocepek</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15900685</idno><idno type="pmid">15900685</idno><idno type="doi">10.3354/dao064029</idno><idno type="wicri:Area/PubMed/Corpus">000538</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000538</idno><idno type="wicri:Area/PubMed/Curation">000538</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000538</idno><idno type="wicri:Area/PubMed/Checkpoint">000538</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000538</idno><idno type="wicri:Area/Ncbi/Merge">000149</idno><idno type="wicri:Area/Ncbi/Curation">000149</idno><idno type="wicri:Area/Ncbi/Checkpoint">000149</idno><idno type="wicri:doubleKey">0177-5103:2005:Pate M:detection:of:mycobacteria</idno><idno type="wicri:Area/Main/Merge">000E21</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods.</title><author><name sortKey="Pate, M" sort="Pate, M" uniqKey="Pate M" first="M" last="Pate">M. Pate</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Faculty Ljubljana, University of Ljubljana, Gerbiceva 60, 1115 Ljubljana, Slovenia.</nlm:affiliation><country xml:lang="fr">Slovénie</country><wicri:regionArea>Veterinary Faculty Ljubljana, University of Ljubljana, Gerbiceva 60, 1115 Ljubljana</wicri:regionArea><wicri:noRegion>1115 Ljubljana</wicri:noRegion></affiliation></author><author><name sortKey="Jencic, V" sort="Jencic, V" uniqKey="Jencic V" first="V" last="Jencic">V. Jencic</name></author><author><name sortKey="Zolnir Dovc, M" sort="Zolnir Dovc, M" uniqKey="Zolnir Dovc M" first="M" last="Zolnir-Dovc">M. Zolnir-Dovc</name></author><author><name sortKey="Ocepek, M" sort="Ocepek, M" uniqKey="Ocepek M" first="M" last="Ocepek">M. Ocepek</name></author></analytic><series><title level="j">Diseases of aquatic organisms</title><idno type="ISSN">0177-5103</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Aquaculture</term><term>Colony Count, Microbial</term><term>Fish Diseases (epidemiology)</term><term>Fish Diseases (microbiology)</term><term>Fishes</term><term>Genotype</term><term>Mycobacterium (genetics)</term><term>Mycobacterium (isolation & purification)</term><term>Mycobacterium Infections (epidemiology)</term><term>Mycobacterium Infections (veterinary)</term><term>Polymorphism, Restriction Fragment Length</term><term>Restriction Mapping</term><term>Slovenia (epidemiology)</term><term>Species Specificity</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Slovenia</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Fish Diseases</term><term>Mycobacterium Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycobacterium</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Mycobacterium</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Mycobacterium Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Aquaculture</term><term>Colony Count, Microbial</term><term>Fishes</term><term>Genotype</term><term>Polymorphism, Restriction Fragment Length</term><term>Restriction Mapping</term><term>Species Specificity</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Slovénie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Thirty-five aquarium fish were investigated for the presence of mycobacteria by culture and molecular methods. The following species were examined: goldfish Carassius auratus auratus, guppy Poecilia reticulata, 4 three-spot gourami Trichogaster trichopterus, dwarf gourami Colisa lalia, Siamese fighting fish Betta splendens, freshwater angelfish Pterophyllum scalare, African cichlid fish Cichlidae spp., cichlid fish Microgeophagus altispinosus, cichlid fish Pseudotropheus lombardoi, blue streak hap Labidochromis caeruleus, sterlet Acipenser ruthenus, southern platyfish Xiphophorus maculatus, and catfish Corydoras spp. Isolates of mycobacteria were obtained in 29 cases (82.9%). Two specimens were positive using Ziehl-Neelsen (ZN) staining, but the cultivation failed. Four specimens were both ZN- and culture-negative. On the basis of GenoType Mycobacterium assay (Hain Life-science) and restriction enzyme analysis of the amplified products (PCR-RFLP), 23 isolates (79.3%) were identified: 7 as Mycobacterium fortuitum, 6 as M. gordonae, 6 as M. marinum, 3 as M. chelonae, and 1 as M. peregrinum. Five isolates remained unidentified (Mycobacterium spp.). One case probably represented a mixed infection (M. marinum/M. fortuitum). Since M. marinum infections are also detected in humans, the significance of mycobacteria in aquarium fish should not be overlooked.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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