Insulin‐like growth factor‐I of pejerrey, Odontesthes bonariensis: cDNA characterization, tissue distribution and expression profiles after growth hormone administration
Identifieur interne : 000B06 ( Main/Merge ); précédent : 000B05; suivant : 000B07Insulin‐like growth factor‐I of pejerrey, Odontesthes bonariensis: cDNA characterization, tissue distribution and expression profiles after growth hormone administration
Auteurs : Andrés A. Sciara [Argentine] ; Gustavo. M. Somoza [Argentine] ; Silvia. E. Arranz [Argentine]Source :
- Journal of Experimental Zoology Part A: Ecological Genetics and Physiology [ 1932-5223 ] ; 2008-08-01.
Abstract
The liver production of the insulin‐like growth factor‐I (IGF‐I) is a key factor in the endocrine control of body growth by a growth hormone. As pejerrey Odontesthes bonariensis has been reported as a fish with low growth rates in captivity, basic research on this respect is needed in order to understand it. In this context, the pejerrey IGF‐I cDNA was cloned and its hepatic expression was examined in fish after recombinant pejerrey growth hormone (pjGHr) administration. The full length of IGF‐I transcript showed a high sequence similarity to other teleost sequences. The tissue distribution analysis by reverse transcriptase polymerase chain reaction in adult fish revealed that IGF‐I expressed ubiquitously with the highest mRNA levels in the liver, posterior intestine and brain. No alternative IGF‐I mRNA was found in the liver, as it was reported for other teleosts. IGF‐I transcript was measured in the liver after pjGHr in vivo stimulation by means of quantitative real‐time polymerase chain reaction assays. A dose‐dependent response of IGF‐I mRNA was observed after pjGHr administration, and reached a six‐fold IGF‐I maximum increase over control group when 2.5 µg pjGH/g‐body weight (bw) was injected. Temporal analysis of hepatic IGF‐I mRNA level showed that administration of a single dose of pjGHr into juvenile pejerrey resulted in a significant increase (P<0.02) 9 hours post‐injection (hpi). These results add novel information on the nucleotide sequence of IGF‐I in Atheriniformes and demonstrate that pjGHr could promote a dramatic response in liver, increasing the IGF‐I mRNA level. J. Exp. Zool. 309A:407–418, 2008. © 2008 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jez.466
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001362
- to stream Istex, to step Curation: 001360
- to stream Istex, to step Checkpoint: 000506
Links to Exploration step
ISTEX:B9E10AD8C5C97F7FC108809ACFF76B1D151892BFLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Insulin‐like growth factor‐I of pejerrey, Odontesthes bonariensis: cDNA characterization, tissue distribution and expression profiles after growth hormone administration</title>
<author><name sortKey="Sciara, Andres A" sort="Sciara, Andres A" uniqKey="Sciara A" first="Andrés A." last="Sciara">Andrés A. Sciara</name>
</author>
<author><name sortKey="Somoza, Gustavo M" sort="Somoza, Gustavo M" uniqKey="Somoza G" first="Gustavo. M." last="Somoza">Gustavo. M. Somoza</name>
</author>
<author><name sortKey="Arranz, Silvia E" sort="Arranz, Silvia E" uniqKey="Arranz S" first="Silvia. E." last="Arranz">Silvia. E. Arranz</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:B9E10AD8C5C97F7FC108809ACFF76B1D151892BF</idno>
<date when="2008" year="2008">2008</date>
<idno type="doi">10.1002/jez.466</idno>
<idno type="url">https://api.istex.fr/document/B9E10AD8C5C97F7FC108809ACFF76B1D151892BF/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001362</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001362</idno>
<idno type="wicri:Area/Istex/Curation">001360</idno>
<idno type="wicri:Area/Istex/Checkpoint">000506</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000506</idno>
<idno type="wicri:doubleKey">1932-5223:2008:Sciara A:insulin:like:growth</idno>
<idno type="wicri:Area/Main/Merge">000B06</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Insulin‐like growth factor‐I of pejerrey, Odontesthes bonariensis: cDNA characterization, tissue distribution and expression profiles after growth hormone administration</title>
<author><name sortKey="Sciara, Andres A" sort="Sciara, Andres A" uniqKey="Sciara A" first="Andrés A." last="Sciara">Andrés A. Sciara</name>
<affiliation wicri:level="1"><country xml:lang="fr">Argentine</country>
<wicri:regionArea>Instituto de Biología Molecular y Celular de Rosario (IBR‐CONICET)—Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Provincia de Santa Fe</wicri:regionArea>
<wicri:noRegion>Provincia de Santa Fe</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Somoza, Gustavo M" sort="Somoza, Gustavo M" uniqKey="Somoza G" first="Gustavo. M." last="Somoza">Gustavo. M. Somoza</name>
<affiliation wicri:level="1"><country xml:lang="fr">Argentine</country>
<wicri:regionArea>Laboratorio de Ictiofisiología y Acuicultura, IIB‐INTECH (CONICET‐Universidad de San Martín), Chascomús, Provincia de Buenos Aires</wicri:regionArea>
<wicri:noRegion>Provincia de Buenos Aires</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Arranz, Silvia E" sort="Arranz, Silvia E" uniqKey="Arranz S" first="Silvia. E." last="Arranz">Silvia. E. Arranz</name>
<affiliation wicri:level="1"><country xml:lang="fr">Argentine</country>
<wicri:regionArea>Instituto de Biología Molecular y Celular de Rosario (IBR‐CONICET)—Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Provincia de Santa Fe</wicri:regionArea>
<wicri:noRegion>Provincia de Santa Fe</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Argentine</country>
<wicri:regionArea>Instituto de Biología Molecular y Celular de Rosario (IBR‐CONICET)—Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Suipacha 531, S2002LRK Santa Fe</wicri:regionArea>
<wicri:noRegion>S2002LRK Santa Fe</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Experimental Zoology Part A: Ecological Genetics and Physiology</title>
<title level="j" type="abbrev">J. Exp. Zool.</title>
<idno type="ISSN">1932-5223</idno>
<idno type="eISSN">1932-5231</idno>
<imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="2008-08-01">2008-08-01</date>
<biblScope unit="volume">309A</biblScope>
<biblScope unit="issue">7</biblScope>
<biblScope unit="page" from="407">407</biblScope>
<biblScope unit="page" to="418">418</biblScope>
</imprint>
<idno type="ISSN">1932-5223</idno>
</series>
<idno type="istex">B9E10AD8C5C97F7FC108809ACFF76B1D151892BF</idno>
<idno type="DOI">10.1002/jez.466</idno>
<idno type="ArticleID">JEZ466</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1932-5223</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The liver production of the insulin‐like growth factor‐I (IGF‐I) is a key factor in the endocrine control of body growth by a growth hormone. As pejerrey Odontesthes bonariensis has been reported as a fish with low growth rates in captivity, basic research on this respect is needed in order to understand it. In this context, the pejerrey IGF‐I cDNA was cloned and its hepatic expression was examined in fish after recombinant pejerrey growth hormone (pjGHr) administration. The full length of IGF‐I transcript showed a high sequence similarity to other teleost sequences. The tissue distribution analysis by reverse transcriptase polymerase chain reaction in adult fish revealed that IGF‐I expressed ubiquitously with the highest mRNA levels in the liver, posterior intestine and brain. No alternative IGF‐I mRNA was found in the liver, as it was reported for other teleosts. IGF‐I transcript was measured in the liver after pjGHr in vivo stimulation by means of quantitative real‐time polymerase chain reaction assays. A dose‐dependent response of IGF‐I mRNA was observed after pjGHr administration, and reached a six‐fold IGF‐I maximum increase over control group when 2.5 µg pjGH/g‐body weight (bw) was injected. Temporal analysis of hepatic IGF‐I mRNA level showed that administration of a single dose of pjGHr into juvenile pejerrey resulted in a significant increase (P<0.02) 9 hours post‐injection (hpi). These results add novel information on the nucleotide sequence of IGF‐I in Atheriniformes and demonstrate that pjGHr could promote a dramatic response in liver, increasing the IGF‐I mRNA level. J. Exp. Zool. 309A:407–418, 2008. © 2008 Wiley‐Liss, Inc.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B06 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000B06 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:B9E10AD8C5C97F7FC108809ACFF76B1D151892BF |texte= Insulin‐like growth factor‐I of pejerrey, Odontesthes bonariensis: cDNA characterization, tissue distribution and expression profiles after growth hormone administration }}
This area was generated with Dilib version V0.6.27. |