Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor® 488 conjugate for visualization of sturgeon acrosome
Identifieur interne : 000A88 ( Main/Merge ); précédent : 000A87; suivant : 000A89Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor® 488 conjugate for visualization of sturgeon acrosome
Auteurs : M. Psenicka [République tchèque] ; J. Cosson [France] ; S. M. H. Alavi [République tchèque] ; M. Rodina [République tchèque] ; V. Kaspar [République tchèque] ; D. Gela [République tchèque] ; O. Linhart [République tchèque] ; A. Ciereszko [Pologne]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2008-08.
Abstract
Trypsin‐like activity, similar to acrosin, is present in sturgeon spermatozoa and can be a potential target for trypsin inhibitors. The objective of this work was to use a fluorescent soybean trypsin inhibitor (SBTI) conjugate with the Alexa Fluor® 488 dye for visualization of the sturgeon acrosome. After incubation with SBTI‐Alexa, a strong signal was observed both in the acrosome and midpiece or implantation fossa region. We have also found that SBTI‐Alexa staining can be combined with PI viability test. Detailed examination of staining pattern revealed that SBTI‐Alexa can stain either acrosome or whole sperm. Staining of whole sperm correlated with dead staining (r2 = 0.94, P < 0.01). However, in fresh semen most cells (93–97%) were not stained with SBTI‐Alexa, probably due to intact acrosomes. Further studies should test if SBTI‐Alexa can be applied to monitor the acrosome status during the acrosome reaction and cryopreservation.
Url:
DOI: 10.1111/j.1439-0426.2008.01140.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000238
- to stream Istex, to step Curation: 000238
- to stream Istex, to step Checkpoint: 000488
Links to Exploration step
ISTEX:D410D188F9BE140E2E47C8262B3AE00BE1D95A10Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor® 488 conjugate for visualization of sturgeon acrosome</title><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M." last="Psenicka">M. Psenicka</name></author><author><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J." last="Cosson">J. Cosson</name></author><author><name sortKey="Alavi, S M H" sort="Alavi, S M H" uniqKey="Alavi S" first="S. M. H." last="Alavi">S. M. H. Alavi</name></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name></author><author><name sortKey="Kaspar, V" sort="Kaspar, V" uniqKey="Kaspar V" first="V." last="Kaspar">V. Kaspar</name></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. Gela</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name></author><author><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A." last="Ciereszko">A. Ciereszko</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D410D188F9BE140E2E47C8262B3AE00BE1D95A10</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1111/j.1439-0426.2008.01140.x</idno><idno type="url">https://api.istex.fr/document/D410D188F9BE140E2E47C8262B3AE00BE1D95A10/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000238</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000238</idno><idno type="wicri:Area/Istex/Curation">000238</idno><idno type="wicri:Area/Istex/Checkpoint">000488</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000488</idno><idno type="wicri:doubleKey">0175-8659:2008:Psenicka M:staining:of:sturgeon</idno><idno type="wicri:Area/Main/Merge">000A88</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor® 488 conjugate for visualization of sturgeon acrosome</title><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M." last="Psenicka">M. Psenicka</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J." last="Cosson">J. Cosson</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>UMR 7009 CNRS – Univ. Paris VI, Observatore de Villefranche/mer, Marine Station, Villefranche‐sur‐mer</wicri:regionArea><wicri:noRegion>Villefranche‐sur‐mer</wicri:noRegion><wicri:noRegion>Villefranche‐sur‐mer</wicri:noRegion></affiliation></author><author><name sortKey="Alavi, S M H" sort="Alavi, S M H" uniqKey="Alavi S" first="S. M. H." last="Alavi">S. M. H. Alavi</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Kaspar, V" sort="Kaspar, V" uniqKey="Kaspar V" first="V." last="Kaspar">V. Kaspar</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. Gela</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A." last="Ciereszko">A. Ciereszko</name><affiliation wicri:level="1"><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn</wicri:regionArea><wicri:noRegion>Olsztyn</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2008-08">2008-08</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="514">514</biblScope><biblScope unit="page" to="516">516</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">D410D188F9BE140E2E47C8262B3AE00BE1D95A10</idno><idno type="DOI">10.1111/j.1439-0426.2008.01140.x</idno><idno type="ArticleID">JAI1140</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trypsin‐like activity, similar to acrosin, is present in sturgeon spermatozoa and can be a potential target for trypsin inhibitors. The objective of this work was to use a fluorescent soybean trypsin inhibitor (SBTI) conjugate with the Alexa Fluor® 488 dye for visualization of the sturgeon acrosome. After incubation with SBTI‐Alexa, a strong signal was observed both in the acrosome and midpiece or implantation fossa region. We have also found that SBTI‐Alexa staining can be combined with PI viability test. Detailed examination of staining pattern revealed that SBTI‐Alexa can stain either acrosome or whole sperm. Staining of whole sperm correlated with dead staining (r2 = 0.94, P < 0.01). However, in fresh semen most cells (93–97%) were not stained with SBTI‐Alexa, probably due to intact acrosomes. Further studies should test if SBTI‐Alexa can be applied to monitor the acrosome status during the acrosome reaction and cryopreservation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A88 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000A88 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:D410D188F9BE140E2E47C8262B3AE00BE1D95A10
|texte= Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor® 488 conjugate for visualization of sturgeon acrosome
}}
| This area was generated with Dilib version V0.6.27. |  |