Comparative proteomics analysis of male and female Persian sturgeon (Acipenser persicus) gonads.
Identifieur interne : 000920 ( Main/Merge ); précédent : 000919; suivant : 000921Comparative proteomics analysis of male and female Persian sturgeon (Acipenser persicus) gonads.
Auteurs : Saeed Keyvanshokooh [Iran] ; Mohammad Reza Kalbassi ; Saman Hosseinkhani ; Behrouz VaziriSource :
- Animal reproduction science [ 1873-2232 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- metabolism : Fishes, Ovary, Testis.
- methods : Proteomics.
- veterinary : Electrophoresis, Gel, Two-Dimensional, Sex Determination Analysis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Animals, Aquaculture, Female, Male.
Abstract
In sturgeon aquaculture, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on a molecular method is of special interest. In the present study a proteomics approach has been utilized to analyze a differential protein expression between mature male and female Persian sturgeon (Acipenser persicus) gonads. When comparing protein patterns on the 2-DE gels of the testis and ovary, 48 unique spots were distinguished in testis while only two spots were matchless in ovary. The spots were identified by MALDI-TOF/TOF analysis. The identified proteins are involved in metabolism and energy production, cell structure, transcription and translation, cell defense, signal transduction, transport, cell division, and none were directly linked to a sex-determining gene. The provided proteomics data could be considered as a starting base for subsequent studies focusing on the identification of proteins involved in sex determination and differentiation at different stages of gonadal maturation.
DOI: 10.1016/j.anireprosci.2008.03.005
PubMed: 18440735
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Development of a non-invasive procedure for sexing fish based on a molecular method is of special interest. In the present study a proteomics approach has been utilized to analyze a differential protein expression between mature male and female Persian sturgeon (Acipenser persicus) gonads. When comparing protein patterns on the 2-DE gels of the testis and ovary, 48 unique spots were distinguished in testis while only two spots were matchless in ovary. The spots were identified by MALDI-TOF/TOF analysis. The identified proteins are involved in metabolism and energy production, cell structure, transcription and translation, cell defense, signal transduction, transport, cell division, and none were directly linked to a sex-determining gene. The provided proteomics data could be considered as a starting base for subsequent studies focusing on the identification of proteins involved in sex determination and differentiation at different stages of gonadal maturation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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