Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals.
Identifieur interne : 000910 ( Main/Merge ); précédent : 000909; suivant : 000911Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals.
Auteurs : Hong Cao [République populaire de Chine] ; Li Zhou ; Yan-Zhen Zhang ; Qi-Wei Wei ; Xi-Hua Chen ; Jian-Fang GuiSource :
- Molecular and cellular endocrinology [ 1872-8057 ] ; 2009.
English descriptors
- KwdEn :
- Age Factors, Animals, Cloning, Molecular, DNA, Complementary, Female, Fishes, Follicle Stimulating Hormone (analysis), Follicle Stimulating Hormone (genetics), Glycoprotein Hormones, alpha Subunit (analysis), Glycoprotein Hormones, alpha Subunit (genetics), Gonadotropins (analysis), Gonadotropins (genetics), Luteinizing Hormone (analysis), Luteinizing Hormone (genetics), Male, Pituitary Gland (chemistry), Sex Factors, Tissue Distribution.
- MESH :
- chemical , analysis : Follicle Stimulating Hormone, Glycoprotein Hormones, alpha Subunit, Gonadotropins, Luteinizing Hormone.
- chemical , genetics : Follicle Stimulating Hormone, Glycoprotein Hormones, alpha Subunit, Gonadotropins, Luteinizing Hormone.
- chemical : DNA, Complementary.
- chemistry : Pituitary Gland.
- Age Factors, Animals, Cloning, Molecular, Female, Fishes, Male, Sex Factors, Tissue Distribution.
Abstract
Chinese sturgeon (Acipenser sinensis) is a rare and endangered species, and also an important resource for the sturgeon aquaculture industry. To understand molecular characterization of Chinese sturgeon gonadotropins (GTHs), we cloned the full-length cDNAs of gonadotropin subunits common alpha (GTH-alpha), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from a pituitary cDNA library of mature female. Two subtypes of GTH-alpha were identified. The nucleotide sequences of A. sinensis common alpha I (AsGTH-alpha I), common alpha II (AsGTH-alpha II), FSHbeta (AsFSHbeta) and LHbeta (AsLHbeta) subunit cDNAs are 345, 363, 387 and 414bp in length, and encode mature peptides of 115, 121, 129 and 138aa, respectively. Then, three polyclonal antibodies were prepared from the in vitro expressed AsGTH-alpha I, AsFSHbeta and AsLHbeta mature proteins, respectively. Significant expression differences were revealed between immature and mature sturgeon pituitaries. Western blot detection and immunofluoresence localization revealed the existence of three-gonadotropin subunits (AsGTH-alpha, AsFSHbeta and AsLHbeta) in mature sturgeon pituitaries, but only AsFSHbeta was detected in immature individual pituitaries during early stages in the sturgeon life, and obvious difference was observed between males and females. In males, AsFSHbeta was expressed in 4-year-old individuals, whereas in females, AsFSHbeta was just expressed in 5-year-old individuals.
DOI: 10.1016/j.mce.2009.01.015
PubMed: 19428989
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Zhou</name></author><author><name sortKey="Zhang, Yan Zhen" sort="Zhang, Yan Zhen" uniqKey="Zhang Y" first="Yan-Zhen" last="Zhang">Yan-Zhen Zhang</name></author><author><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name></author><author><name sortKey="Chen, Xi Hua" sort="Chen, Xi Hua" uniqKey="Chen X" first="Xi-Hua" last="Chen">Xi-Hua Chen</name></author><author><name sortKey="Gui, Jian Fang" sort="Gui, Jian Fang" uniqKey="Gui J" first="Jian-Fang" last="Gui">Jian-Fang Gui</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19428989</idno><idno type="pmid">19428989</idno><idno type="doi">10.1016/j.mce.2009.01.015</idno><idno type="wicri:Area/PubMed/Corpus">000431</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000431</idno><idno type="wicri:Area/PubMed/Curation">000431</idno><idno 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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Li" sort="Zhou, Li" uniqKey="Zhou L" first="Li" last="Zhou">Li Zhou</name></author><author><name sortKey="Zhang, Yan Zhen" sort="Zhang, Yan Zhen" uniqKey="Zhang Y" first="Yan-Zhen" last="Zhang">Yan-Zhen Zhang</name></author><author><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name></author><author><name sortKey="Chen, Xi Hua" sort="Chen, Xi Hua" uniqKey="Chen X" first="Xi-Hua" last="Chen">Xi-Hua Chen</name></author><author><name sortKey="Gui, Jian Fang" sort="Gui, Jian Fang" uniqKey="Gui J" first="Jian-Fang" last="Gui">Jian-Fang 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qualifier="analysis" xml:lang="en"><term>Follicle Stimulating Hormone</term><term>Glycoprotein Hormones, alpha Subunit</term><term>Gonadotropins</term><term>Luteinizing Hormone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Follicle Stimulating Hormone</term><term>Glycoprotein Hormones, alpha Subunit</term><term>Gonadotropins</term><term>Luteinizing Hormone</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Pituitary Gland</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Age Factors</term><term>Animals</term><term>Cloning, Molecular</term><term>Female</term><term>Fishes</term><term>Male</term><term>Sex Factors</term><term>Tissue Distribution</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chinese sturgeon (Acipenser sinensis) is a rare and endangered species, and also an important resource for the sturgeon aquaculture industry. To understand molecular characterization of Chinese sturgeon gonadotropins (GTHs), we cloned the full-length cDNAs of gonadotropin subunits common alpha (GTH-alpha), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from a pituitary cDNA library of mature female. Two subtypes of GTH-alpha were identified. The nucleotide sequences of A. sinensis common alpha I (AsGTH-alpha I), common alpha II (AsGTH-alpha II), FSHbeta (AsFSHbeta) and LHbeta (AsLHbeta) subunit cDNAs are 345, 363, 387 and 414bp in length, and encode mature peptides of 115, 121, 129 and 138aa, respectively. Then, three polyclonal antibodies were prepared from the in vitro expressed AsGTH-alpha I, AsFSHbeta and AsLHbeta mature proteins, respectively. Significant expression differences were revealed between immature and mature sturgeon pituitaries. Western blot detection and immunofluoresence localization revealed the existence of three-gonadotropin subunits (AsGTH-alpha, AsFSHbeta and AsLHbeta) in mature sturgeon pituitaries, but only AsFSHbeta was detected in immature individual pituitaries during early stages in the sturgeon life, and obvious difference was observed between males and females. In males, AsFSHbeta was expressed in 4-year-old individuals, whereas in females, AsFSHbeta was just expressed in 5-year-old individuals.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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