EsturgeonV1, Main, Merge, bibRecord, 000901

Testing taxonomic boundaries and the limit of DNA barcoding in the Siberian sturgeon, Acipenser baerii.

Identifieur interne : 000901 ( Main/Merge ); précédent : 000900; suivant : 000902

Testing taxonomic boundaries and the limit of DNA barcoding in the Siberian sturgeon, Acipenser baerii.

Auteurs : Vadim J. Birstein [États-Unis] ; Rob Desalle ; Phaedra Doukakis ; Robert Hanner ; Georgii I. Ruban ; Eugene Wong

Source :

Mitochondrial DNA [ 1940-1744 ] ; 2009.

RBID : pubmed:19900060

English descriptors

KwdEn :
- Animals, Base Sequence, DNA, Mitochondrial (chemistry), Fishes (classification), Fishes (genetics), Genetic Variation, Genetics, Population, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Species Specificity.
MESH :
- chemical , chemistry : DNA, Mitochondrial.
- classification : Fishes.
- genetics : Fishes.
- Animals, Base Sequence, Genetic Variation, Genetics, Population, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Species Specificity.

Abstract

DNA barcoding efforts involving animals have focused on the mitochondrial cytochrome c oxidase subunit I (Cox1) gene. Some authors suggest that this marker might under-diagnose young species. Herein, we examine Cox1 and control region diversity in a sample of Siberian sturgeon (Acipenser baerii), a species with an extremely wide geographic distribution in the major rivers of Siberia and in Lake Baikal. Some authors currently recognize three subspecies within this species. These subspecies are reasonable candidates for species units detectable through DNA barcoding. The Cox1 gene illustrated no variation within the species, while the control region displayed statistically significant differences among the subspecies using analysis of molecular variance (AMOVA). Given the uniformity of Cox1 sequences recovered, Cox1 is probably a good region for barcoding A. baerii at the species level. Although control region variation among subspecies was significant, diagnostic differences were not found for any of the subspecies.

DOI: 10.3109/19401730903168182
PubMed: 19900060

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pubmed:19900060

Le document en format XML

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<author><name sortKey="Doukakis, Phaedra" sort="Doukakis, Phaedra" uniqKey="Doukakis P" first="Phaedra" last="Doukakis">Phaedra Doukakis</name>
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<author><name sortKey="Hanner, Robert" sort="Hanner, Robert" uniqKey="Hanner R" first="Robert" last="Hanner">Robert Hanner</name>
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<term>Genetic Variation</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">DNA barcoding efforts involving animals have focused on the mitochondrial cytochrome c oxidase subunit I (Cox1) gene. Some authors suggest that this marker might under-diagnose young species. Herein, we examine Cox1 and control region diversity in a sample of Siberian sturgeon (Acipenser baerii), a species with an extremely wide geographic distribution in the major rivers of Siberia and in Lake Baikal. Some authors currently recognize three subspecies within this species. These subspecies are reasonable candidates for species units detectable through DNA barcoding. The Cox1 gene illustrated no variation within the species, while the control region displayed statistically significant differences among the subspecies using analysis of molecular variance (AMOVA). Given the uniformity of Cox1 sequences recovered, Cox1 is probably a good region for barcoding A. baerii at the species level. Although control region variation among subspecies was significant, diagnostic differences were not found for any of the subspecies.</div>
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Testing taxonomic boundaries and the limit of DNA barcoding in the Siberian sturgeon, Acipenser baerii.

Testing taxonomic boundaries and the limit of DNA barcoding in the Siberian sturgeon, Acipenser baerii.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki