A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium
Identifieur interne : 000892 ( Main/Merge ); précédent : 000891; suivant : 000893A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium
Auteurs : Frédéric Silvestre [États-Unis, Belgique] ; Javier Linares-Casenave [États-Unis] ; Serge I. Doroshov [États-Unis] ; Dietmar Kültz [États-Unis]Source :
- Science of the total environment [ 0048-9697 ] ; 2010.
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- Pascal (Inist)
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Abstract
Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 °C and micro-injected with Seleno-L-Methionine to reach 8 μg g-1 selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P<0.01). Gel replicates were first grouped according to heat treatment. Fifteen of these spots were identified using MALDI TOF/TOF mass spectrometry. Proteins involved in protein folding, protein synthesis, protein degradation, ATP supply and structural proteins changed in abundance in response to heat and/or selenium. 40S ribosomal protein SA, FK506-binding protein 10, 65 kDa regulatory subunit A of protein phosphatase 2, protein disulfide isomerase, stress-induced-phosphoprotein 1, suppression of tumorigenicity 13 and collagen type II alpha 1, were differentially expressed in high temperature treatment only. Serine/ arginine repetitive matrix protein 1, creatine kinase, serine peptidase inhibitor Kazal type 5 and HSP90 were sensitive to combined temperature and selenium exposure. Valosin-containing protein, a protein involved in aggresome formation and in protein quality control decreased more than 50% in response to selenium treatment. Potential use of such proteins as biomarkers of environmental stressors in larval sturgeons could indicate early warning signals preceding population decline.
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To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 °C and micro-injected with Seleno-L-Methionine to reach 8 μg g<sup>-1</sup> selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (P<0.01). Gel replicates were first grouped according to heat treatment. Fifteen of these spots were identified using MALDI TOF/TOF mass spectrometry. Proteins involved in protein folding, protein synthesis, protein degradation, ATP supply and structural proteins changed in abundance in response to heat and/or selenium. 40S ribosomal protein SA, FK506-binding protein 10, 65 kDa regulatory subunit A of protein phosphatase 2, protein disulfide isomerase, stress-induced-phosphoprotein 1, suppression of tumorigenicity 13 and collagen type II alpha 1, were differentially expressed in high temperature treatment only. Serine/ arginine repetitive matrix protein 1, creatine kinase, serine peptidase inhibitor Kazal type 5 and HSP90 were sensitive to combined temperature and selenium exposure. Valosin-containing protein, a protein involved in aggresome formation and in protein quality control decreased more than 50% in response to selenium treatment. Potential use of such proteins as biomarkers of environmental stressors in larval sturgeons could indicate early warning signals preceding population decline.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Silvestre, Frederic" sort="Silvestre, Frederic" uniqKey="Silvestre F" first="Frédéric" last="Silvestre">Frédéric Silvestre</name></region><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I." last="Doroshov">Serge I. Doroshov</name><name sortKey="Kultz, Dietmar" sort="Kultz, Dietmar" uniqKey="Kultz D" first="Dietmar" last="Kültz">Dietmar Kültz</name><name sortKey="Linares Casenave, Javier" sort="Linares Casenave, Javier" uniqKey="Linares Casenave J" first="Javier" last="Linares-Casenave">Javier Linares-Casenave</name></country><country name="Belgique"><noRegion><name sortKey="Silvestre, Frederic" sort="Silvestre, Frederic" uniqKey="Silvestre F" first="Frédéric" last="Silvestre">Frédéric Silvestre</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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