Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus)
Identifieur interne : 000523 ( Main/Merge ); précédent : 000522; suivant : 000524Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus)
Auteurs : A. Shaliutina [République tchèque] ; B. Dzyuba [République tchèque, Ukraine] ; M. Hulak [République tchèque] ; S. Boryshpolets [République tchèque] ; P. Li [République tchèque] ; O. Linhart [République tchèque]Source :
- Reproduction in Domestic Animals [ 0936-6768 ] ; 2012-06.
English descriptors
- KwdEn :
- MESH :
- methods : Semen Analysis.
- physiology : Fishes, Semen, Spermatozoa.
- veterinary : Semen Analysis.
- Animals, Endangered Species, Male, Osmolar Concentration, Sperm Motility.
Abstract
This study investigated the effects of multiple collections of sperm on endangered sterlet (Acipenser ruthenus) sperm functional parameters [spermatozoa motility and curvilinear velocity (VCL)] as well as on protein concentration and osmolality of seminal plasma. The average sperm volume and mean spermatozoa concentration per male were significantly altered with multiple collections. On the other hand, no significant effect of multiple collections on protein concentration of seminal plasma was observed. In all experimental groups, moderate impact of sequential collection on osmolality (p < 0.05) of seminal plasma was observed. Ninety to 100% of motile spermatozoa were observed at 15 s after activation, with an average VCL of 181.12 ± 19.10 μm/s. After 90 s, average VCL decreased to 130 ± 26 μm/s. Motility was maintained for up to 4 min. The maximum percentage of motile spermatozoa was observed after the third collection of sperm. The spermatozoa VCL increased significantly with subsequent collections. The results of this study provide new data on the effects of multiple collections on quantitative and qualitative parameters of sperm in sterlet. The data confirmed that the sequential stripping has no negative effect on the percentage of motility and spermatozoa velocity. This should be beneficial for the development of sterlet aquaculture programs.
Url:
DOI: 10.1111/j.1439-0531.2011.01907.x
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Li</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Reproduction in Domestic Animals</title><idno type="ISSN">0936-6768</idno><idno type="eISSN">1439-0531</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06">2012-06</date><biblScope unit="volume">47</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="479">479</biblScope><biblScope unit="page" to="484">484</biblScope></imprint><idno type="ISSN">0936-6768</idno></series><idno type="istex">97A45F76A1364C54F919A6981565FF277139348E</idno><idno type="DOI">10.1111/j.1439-0531.2011.01907.x</idno><idno type="ArticleID">RDA1907</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0936-6768</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Endangered Species</term><term>Fishes (physiology)</term><term>Male</term><term>Osmolar Concentration</term><term>Semen (physiology)</term><term>Semen Analysis (methods)</term><term>Semen Analysis (veterinary)</term><term>Sperm Motility</term><term>Spermatozoa (physiology)</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Semen Analysis</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term><term>Semen</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Semen Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Endangered Species</term><term>Male</term><term>Osmolar Concentration</term><term>Sperm Motility</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study investigated the effects of multiple collections of sperm on endangered sterlet (Acipenser ruthenus) sperm functional parameters [spermatozoa motility and curvilinear velocity (VCL)] as well as on protein concentration and osmolality of seminal plasma. The average sperm volume and mean spermatozoa concentration per male were significantly altered with multiple collections. On the other hand, no significant effect of multiple collections on protein concentration of seminal plasma was observed. In all experimental groups, moderate impact of sequential collection on osmolality (p < 0.05) of seminal plasma was observed. Ninety to 100% of motile spermatozoa were observed at 15 s after activation, with an average VCL of 181.12 ± 19.10 μm/s. After 90 s, average VCL decreased to 130 ± 26 μm/s. Motility was maintained for up to 4 min. The maximum percentage of motile spermatozoa was observed after the third collection of sperm. The spermatozoa VCL increased significantly with subsequent collections. The results of this study provide new data on the effects of multiple collections on quantitative and qualitative parameters of sperm in sterlet. The data confirmed that the sequential stripping has no negative effect on the percentage of motility and spermatozoa velocity. This should be beneficial for the development of sterlet aquaculture programs.</div></front></TEI><double doi="10.1111/j.1439-0531.2011.01907.x"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus)</title><author><name sortKey="Shaliutina, A" sort="Shaliutina, A" uniqKey="Shaliutina A" first="A" last="Shaliutina">A. Shaliutina</name></author><author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M" last="Hulak">M. Hulak</name></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S" last="Boryshpolets">S. Boryshpolets</name></author><author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P" last="Li">P. Li</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:97A45F76A1364C54F919A6981565FF277139348E</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1111/j.1439-0531.2011.01907.x</idno><idno type="url">https://api.istex.fr/document/97A45F76A1364C54F919A6981565FF277139348E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000933</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000933</idno><idno type="wicri:Area/Istex/Curation">000931</idno><idno type="wicri:Area/Istex/Checkpoint">000147</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000147</idno><idno type="wicri:doubleKey">0936-6768:2012:Shaliutina A:evaluation:of:spermiation</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus)</title><author><name sortKey="Shaliutina, A" sort="Shaliutina, A" uniqKey="Shaliutina A" first="A" last="Shaliutina">A. Shaliutina</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Ukraine</country><wicri:regionArea>Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov</wicri:regionArea><wicri:noRegion>Kharkov</wicri:noRegion></affiliation></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M" last="Hulak">M. Hulak</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S" last="Boryshpolets">S. Boryshpolets</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P" last="Li">P. Li</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Reproduction in Domestic Animals</title><idno type="ISSN">0936-6768</idno><idno type="eISSN">1439-0531</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06">2012-06</date><biblScope unit="volume">47</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="479">479</biblScope><biblScope unit="page" to="484">484</biblScope></imprint><idno type="ISSN">0936-6768</idno></series><idno type="istex">97A45F76A1364C54F919A6981565FF277139348E</idno><idno type="DOI">10.1111/j.1439-0531.2011.01907.x</idno><idno type="ArticleID">RDA1907</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0936-6768</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study investigated the effects of multiple collections of sperm on endangered sterlet (Acipenser ruthenus) sperm functional parameters [spermatozoa motility and curvilinear velocity (VCL)] as well as on protein concentration and osmolality of seminal plasma. The average sperm volume and mean spermatozoa concentration per male were significantly altered with multiple collections. On the other hand, no significant effect of multiple collections on protein concentration of seminal plasma was observed. In all experimental groups, moderate impact of sequential collection on osmolality (p < 0.05) of seminal plasma was observed. Ninety to 100% of motile spermatozoa were observed at 15 s after activation, with an average VCL of 181.12 ± 19.10 μm/s. After 90 s, average VCL decreased to 130 ± 26 μm/s. Motility was maintained for up to 4 min. The maximum percentage of motile spermatozoa was observed after the third collection of sperm. The spermatozoa VCL increased significantly with subsequent collections. The results of this study provide new data on the effects of multiple collections on quantitative and qualitative parameters of sperm in sterlet. The data confirmed that the sequential stripping has no negative effect on the percentage of motility and spermatozoa velocity. This should be beneficial for the development of sterlet aquaculture programs.</div></front></TEI></ISTEX><PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of spermiation indices with multiple sperm collections in endangered sterlet (Acipenser ruthenus).</title><author><name sortKey="Shaliutina, A" sort="Shaliutina, A" uniqKey="Shaliutina A" first="A" last="Shaliutina">A. Shaliutina</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic. a_shalutyna@mail.ru</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M" last="Hulak">M. Hulak</name></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S" last="Boryshpolets">S. Boryshpolets</name></author><author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P" last="Li">P. Li</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:21978021</idno><idno type="pmid">21978021</idno><idno type="doi">10.1111/j.1439-0531.2011.01907.x</idno><idno type="wicri:Area/PubMed/Corpus">000314</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000314</idno><idno type="wicri:Area/PubMed/Curation">000314</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000314</idno><idno type="wicri:Area/PubMed/Checkpoint">000314</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000314</idno><idno type="wicri:Area/Ncbi/Merge">000402</idno><idno type="wicri:Area/Ncbi/Curation">000402</idno><idno type="wicri:Area/Ncbi/Checkpoint">000402</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of spermiation indices with multiple sperm collections in endangered sterlet (Acipenser ruthenus).</title><author><name sortKey="Shaliutina, A" sort="Shaliutina, A" uniqKey="Shaliutina A" first="A" last="Shaliutina">A. Shaliutina</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic. a_shalutyna@mail.ru</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Dzyuba, B" sort="Dzyuba, B" uniqKey="Dzyuba B" first="B" last="Dzyuba">B. Dzyuba</name></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M" last="Hulak">M. Hulak</name></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S" last="Boryshpolets">S. Boryshpolets</name></author><author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P" last="Li">P. Li</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name></author></analytic><series><title level="j">Reproduction in domestic animals = Zuchthygiene</title><idno type="eISSN">1439-0531</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Endangered Species</term><term>Fishes (physiology)</term><term>Male</term><term>Osmolar Concentration</term><term>Semen (physiology)</term><term>Semen Analysis (methods)</term><term>Semen Analysis (veterinary)</term><term>Sperm Motility</term><term>Spermatozoa (physiology)</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Semen Analysis</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term><term>Semen</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Semen Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Endangered Species</term><term>Male</term><term>Osmolar Concentration</term><term>Sperm Motility</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study investigated the effects of multiple collections of sperm on endangered sterlet (Acipenser ruthenus) sperm functional parameters [spermatozoa motility and curvilinear velocity (VCL)] as well as on protein concentration and osmolality of seminal plasma. The average sperm volume and mean spermatozoa concentration per male were significantly altered with multiple collections. On the other hand, no significant effect of multiple collections on protein concentration of seminal plasma was observed. In all experimental groups, moderate impact of sequential collection on osmolality (p < 0.05) of seminal plasma was observed. Ninety to 100% of motile spermatozoa were observed at 15 s after activation, with an average VCL of 181.12 ± 19.10 μm/s. After 90 s, average VCL decreased to 130 ± 26 μm/s. Motility was maintained for up to 4 min. The maximum percentage of motile spermatozoa was observed after the third collection of sperm. The spermatozoa VCL increased significantly with subsequent collections. The results of this study provide new data on the effects of multiple collections on quantitative and qualitative parameters of sperm in sterlet. The data confirmed that the sequential stripping has no negative effect on the percentage of motility and spermatozoa velocity. This should be beneficial for the development of sterlet aquaculture programs.</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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