Lactococcus lactis subsp. lactis infection in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus, in Taiwan.
Identifieur interne : 000443 ( Main/Merge ); précédent : 000442; suivant : 000444Lactococcus lactis subsp. lactis infection in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus, in Taiwan.
Auteurs : Ming-Hui Chen [Taïwan] ; Shao-Wen Hung ; Ching-Lin Shyu ; Cheng-Chung Lin ; Pan-Chen Liu ; Chen-Hsuan Chang ; Wei-Yau Shia ; Ching-Fu Cheng ; Shiun-Long Lin ; Ching-Yu Tu ; Yu-Hsing Lin ; Way-Shyan WangSource :
- Research in veterinary science [ 1532-2661 ] ; 2012.
Descripteurs français
- Wicri :
- geographic : Taïwan.
English descriptors
- KwdEn :
- Animals, Aquaculture, Base Sequence, Fish Diseases (epidemiology), Fish Diseases (microbiology), Fish Diseases (pathology), Fishes (genetics), Gram-Positive Bacterial Infections (epidemiology), Gram-Positive Bacterial Infections (microbiology), Gram-Positive Bacterial Infections (pathology), Gram-Positive Bacterial Infections (veterinary), Lactococcus lactis (isolation & purification), Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction (veterinary), RNA, Bacterial (genetics), RNA, Ribosomal, 16S (genetics), Taiwan (epidemiology), Tilapia (genetics).
- MESH :
- chemical , genetics : RNA, Bacterial, RNA, Ribosomal, 16S.
- geographic , epidemiology : Taiwan.
- epidemiology : Fish Diseases, Gram-Positive Bacterial Infections.
- genetics : Fishes, Tilapia.
- isolation & purification : Lactococcus lactis.
- microbiology : Fish Diseases, Gram-Positive Bacterial Infections.
- pathology : Fish Diseases, Gram-Positive Bacterial Infections.
- veterinary : Gram-Positive Bacterial Infections, Polymerase Chain Reaction.
- Animals, Aquaculture, Base Sequence, Molecular Sequence Data, Phylogeny.
Abstract
Approximately 5300 hybrid sturgeons with an average body weight of 600-800 g were farmed in 3 round tankers measuring 3m in diameter each containing 28,000 L of aerated groundwater. According to the owner's description, the diseased fish had anorexia, pale body color, and reddish spots on the abdomen. The morbidity and lethality rates in this outbreak were about 70% (3706/5300) and 100% (3706/3706), respectively. The clinical examination revealed enteritis, enlarged abdomen, and rapid respiration rate. The gross findings revealed a volume of about 4 mL of ascites. The histopathological examination showed multiple massive, hemorrhagic or coagulative necrotic foci in the liver and spleen. Furthermore, there was diffuse infiltration of glycogen in hepatic cells, and a few polymorphonuclear and mononuclear leucocytes were observed surrounding the spleen. Some bacterial clumps were noted around the necrotic foci. We also observed that there was moderate to severe, acute, multifocal, coagulative necrosis in the renal parenchyma, with some necrotic foci present beneath the margin of the kidney. Additionally, multifocal, coagulative necrosis was found in the pancreas. Results of microbiologic examinations, including biochemical characteristics, PCR amplification of 16S rRNA gene, sequencing and comparison, and phylogenetic analysis, revealed the pathogen of this infection was Lactococcus lactis subsp. lactis, and based on the results of an antimicrobial agent sensitivity test the bacterium was only sensitive to ampicillin and florfenicol. Additionally, results of in vivo experimental infections in hybrid tilapia showed that 1×10(8) and 1×10(9) CFU/mL of our isolate caused death in all fish and LD(50) values ranged from 10(2) to 10(5) CFU/mL. To the best of the authors' knowledge, this is the first reported case of Lactococcus lactis subsp. lactis infection in hybrid sturgeon.
DOI: 10.1016/j.rvsc.2011.10.007
PubMed: 22098776
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pubmed:22098776Le document en format XML
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xml:lang="en">Approximately 5300 hybrid sturgeons with an average body weight of 600-800 g were farmed in 3 round tankers measuring 3m in diameter each containing 28,000 L of aerated groundwater. According to the owner's description, the diseased fish had anorexia, pale body color, and reddish spots on the abdomen. The morbidity and lethality rates in this outbreak were about 70% (3706/5300) and 100% (3706/3706), respectively. The clinical examination revealed enteritis, enlarged abdomen, and rapid respiration rate. The gross findings revealed a volume of about 4 mL of ascites. The histopathological examination showed multiple massive, hemorrhagic or coagulative necrotic foci in the liver and spleen. Furthermore, there was diffuse infiltration of glycogen in hepatic cells, and a few polymorphonuclear and mononuclear leucocytes were observed surrounding the spleen. Some bacterial clumps were noted around the necrotic foci. We also observed that there was moderate to severe, acute, multifocal, coagulative necrosis in the renal parenchyma, with some necrotic foci present beneath the margin of the kidney. Additionally, multifocal, coagulative necrosis was found in the pancreas. Results of microbiologic examinations, including biochemical characteristics, PCR amplification of 16S rRNA gene, sequencing and comparison, and phylogenetic analysis, revealed the pathogen of this infection was Lactococcus lactis subsp. lactis, and based on the results of an antimicrobial agent sensitivity test the bacterium was only sensitive to ampicillin and florfenicol. Additionally, results of in vivo experimental infections in hybrid tilapia showed that 1×10(8) and 1×10(9) CFU/mL of our isolate caused death in all fish and LD(50) values ranged from 10(2) to 10(5) CFU/mL. To the best of the authors' knowledge, this is the first reported case of Lactococcus lactis subsp. lactis infection in hybrid sturgeon.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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