Relationships between radial glial progenitors and 5‐HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
Identifieur interne : 000326 ( Main/Merge ); précédent : 000325; suivant : 000327Relationships between radial glial progenitors and 5‐HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
Auteurs : María Rita Pérez [France, Argentine] ; Elisabeth Pellegrini [France] ; Joel Cano-Nicolau [France] ; Marie-Madeleine Gueguen [France] ; Dounia Menouer-Le Guillou [France] ; Yohann Merot [France] ; Colette Vaillant [France] ; Gustavo M. Somoza [Argentine] ; Olivier Kah [France]Source :
- European Journal of Neuroscience [ 0953-816X ] ; 2013-11.
Abstract
In non‐mammalian vertebrates, serotonin (5‐HT)‐producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)‐contacting neurons exhibiting 5‐HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5‐HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5‐HT CSF‐contacting neurons, we performed 5‐HT immunohistochemistry in transgenic tg(cyp19a1b‐GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5‐HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5‐HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5‐bromo‐2′‐deoxyuridine indicate that RGCs proliferate and give birth to 5‐HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5‐HT appears to promote genesis of 5‐HT neurons that accumulate along the brain ventricles and contact the CSF.
Url:
DOI: 10.1111/ejn.12348
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001788
- to stream Istex, to step Curation: 001786
- to stream Istex, to step Checkpoint: 000017
Links to Exploration step
ISTEX:0E32753B12A96A08653C53B0FB7DABC5C0F2AFD5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Relationships between radial glial progenitors and 5‐HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis</title><author><name sortKey="Perez, Maria Rita" sort="Perez, Maria Rita" uniqKey="Perez M" first="María Rita" last="Pérez">María Rita Pérez</name></author><author><name sortKey="Pellegrini, Elisabeth" sort="Pellegrini, Elisabeth" uniqKey="Pellegrini E" first="Elisabeth" last="Pellegrini">Elisabeth Pellegrini</name></author><author><name sortKey="Cano Icolau, Joel" sort="Cano Icolau, Joel" uniqKey="Cano Icolau J" first="Joel" last="Cano-Nicolau">Joel Cano-Nicolau</name></author><author><name sortKey="Gueguen, Marie Adeleine" sort="Gueguen, Marie Adeleine" uniqKey="Gueguen M" first="Marie-Madeleine" last="Gueguen">Marie-Madeleine Gueguen</name></author><author><name sortKey="Menouer E Guillou, Dounia" sort="Menouer E Guillou, Dounia" uniqKey="Menouer E Guillou D" first="Dounia" last="Menouer-Le Guillou">Dounia Menouer-Le Guillou</name></author><author><name sortKey="Merot, Yohann" sort="Merot, Yohann" uniqKey="Merot Y" first="Yohann" last="Merot">Yohann Merot</name></author><author><name sortKey="Vaillant, Colette" sort="Vaillant, Colette" uniqKey="Vaillant C" first="Colette" last="Vaillant">Colette Vaillant</name></author><author><name sortKey="Somoza, Gustavo M" sort="Somoza, Gustavo M" uniqKey="Somoza G" first="Gustavo M." last="Somoza">Gustavo M. Somoza</name></author><author><name sortKey="Kah, Olivier" sort="Kah, Olivier" uniqKey="Kah O" first="Olivier" last="Kah">Olivier Kah</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0E32753B12A96A08653C53B0FB7DABC5C0F2AFD5</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/ejn.12348</idno><idno type="url">https://api.istex.fr/document/0E32753B12A96A08653C53B0FB7DABC5C0F2AFD5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001788</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001788</idno><idno type="wicri:Area/Istex/Curation">001786</idno><idno type="wicri:Area/Istex/Checkpoint">000017</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000017</idno><idno type="wicri:doubleKey">0953-816X:2013:Perez M:relationships:between:radial</idno><idno type="wicri:Area/Main/Merge">000326</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Relationships between radial glial progenitors and 5‐HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis</title><author><name sortKey="Perez, Maria Rita" sort="Perez, Maria Rita" uniqKey="Perez M" first="María Rita" last="Pérez">María Rita Pérez</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (IIB‐INTECH. CONICET‐UNSAM), Chascomús</wicri:regionArea><wicri:noRegion>Chascomús</wicri:noRegion></affiliation></author><author><name sortKey="Pellegrini, Elisabeth" sort="Pellegrini, Elisabeth" uniqKey="Pellegrini E" first="Elisabeth" last="Pellegrini">Elisabeth Pellegrini</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Cano Icolau, Joel" sort="Cano Icolau, Joel" uniqKey="Cano Icolau J" first="Joel" last="Cano-Nicolau">Joel Cano-Nicolau</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Gueguen, Marie Adeleine" sort="Gueguen, Marie Adeleine" uniqKey="Gueguen M" first="Marie-Madeleine" last="Gueguen">Marie-Madeleine Gueguen</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Menouer E Guillou, Dounia" sort="Menouer E Guillou, Dounia" uniqKey="Menouer E Guillou D" first="Dounia" last="Menouer-Le Guillou">Dounia Menouer-Le Guillou</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Merot, Yohann" sort="Merot, Yohann" uniqKey="Merot Y" first="Yohann" last="Merot">Yohann Merot</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Vaillant, Colette" sort="Vaillant, Colette" uniqKey="Vaillant C" first="Colette" last="Vaillant">Colette Vaillant</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Somoza, Gustavo M" sort="Somoza, Gustavo M" uniqKey="Somoza G" first="Gustavo M." last="Somoza">Gustavo M. Somoza</name><affiliation wicri:level="1"><country xml:lang="fr">Argentine</country><wicri:regionArea>Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (IIB‐INTECH. CONICET‐UNSAM), Chascomús</wicri:regionArea><wicri:noRegion>Chascomús</wicri:noRegion></affiliation></author><author><name sortKey="Kah, Olivier" sort="Kah, Olivier" uniqKey="Kah O" first="Olivier" last="Kah">Olivier Kah</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Neuroendocrine Effects of Endocrine Disruptors, IRSET, Case 1302, INSERM U1085, Université de Rennes 1, Campus de Beaulieu, 35 042, Rennes cedex</wicri:regionArea><orgName type="university">Université de Rennes 1</orgName><placeName><settlement type="city">Rennes</settlement><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j">European Journal of Neuroscience</title><title level="j" type="abbrev">Eur J Neurosci</title><idno type="ISSN">0953-816X</idno><idno type="eISSN">1460-9568</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-11">2013-11</date><biblScope unit="volume">38</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="3292">3292</biblScope><biblScope unit="page" to="3301">3301</biblScope></imprint><idno type="ISSN">0953-816X</idno></series><idno type="istex">0E32753B12A96A08653C53B0FB7DABC5C0F2AFD5</idno><idno type="DOI">10.1111/ejn.12348</idno><idno type="ArticleID">EJN12348</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0953-816X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">In non‐mammalian vertebrates, serotonin (5‐HT)‐producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)‐contacting neurons exhibiting 5‐HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5‐HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle. To study relationships between RGCs and 5‐HT CSF‐contacting neurons, we performed 5‐HT immunohistochemistry in transgenic tg(cyp19a1b‐GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter. We show that the somata of the 5‐HT neurons are located closer to the ventricle than those of RGCs. RGCs extend towards the ventricle cytoplasmic processes that form a continuous barrier along the ventricular surface. In turn, 5‐HT neurons contact the CSF via processes that cross this barrier through small pores. Further experiments using proliferating cell nuclear antigen or 5‐bromo‐2′‐deoxyuridine indicate that RGCs proliferate and give birth to 5‐HT neurons migrating centripetally instead of centrifugally as in other brain regions. Furthermore, treatment of adult zebrafish with tryptophan hydroxylase inhibitor causes a significant decrease in the number of proliferating cells in the PVO, but not in the mediobasal hypothalamus. These data point to the PVO as an intriguing region in which 5‐HT appears to promote genesis of 5‐HT neurons that accumulate along the brain ventricles and contact the CSF.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000326 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000326 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:0E32753B12A96A08653C53B0FB7DABC5C0F2AFD5
|texte= Relationships between radial glial progenitors and 5‐HT neurons in the paraventricular organ of adult zebrafish – potential effects of serotonin on adult neurogenesis
}}
| This area was generated with Dilib version V0.6.27. |  |