A RING finger protein 114 (RNF114) homolog from Chinese sturgeon (Acipenser sinensis) possesses immune-regulation properties via modulating RIG-I signaling pathway-mediated interferon expression.
Identifieur interne : 000224 ( Main/Merge ); précédent : 000223; suivant : 000225A RING finger protein 114 (RNF114) homolog from Chinese sturgeon (Acipenser sinensis) possesses immune-regulation properties via modulating RIG-I signaling pathway-mediated interferon expression.
Auteurs : Zhiyong Liao [République populaire de Chine] ; Xiaojun Chen [République populaire de Chine] ; Dongsong Nie [République populaire de Chine] ; Jiajia Wang [République populaire de Chine] ; Mingjiang Wu [République populaire de Chine]Source :
- Fish & shellfish immunology [ 1095-9947 ] ; 2014.
English descriptors
- KwdEn :
- Analysis of Variance, Animals, Cloning, Molecular, Computational Biology, DNA Primers (genetics), Fishes (genetics), Fishes (immunology), Gene Knockdown Techniques, Gene Library, HEK293 Cells, Humans, Immunoprecipitation, Interferons (metabolism), NF-kappa B (metabolism), Open Reading Frames (genetics), Phylogeny, Poly I-C, RING Finger Domains (genetics), RING Finger Domains (immunology), Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction (immunology), Ubiquitin-Protein Ligases (genetics), Ubiquitin-Protein Ligases (immunology), Ubiquitin-Protein Ligases (metabolism).
- MESH :
- chemical , genetics : DNA Primers, Ubiquitin-Protein Ligases.
- genetics : Fishes, Open Reading Frames, RING Finger Domains.
- immunology : Fishes, RING Finger Domains, Signal Transduction, Ubiquitin-Protein Ligases.
- chemical , metabolism : Interferons, NF-kappa B, Ubiquitin-Protein Ligases.
- Analysis of Variance, Animals, Cloning, Molecular, Computational Biology, Gene Knockdown Techniques, Gene Library, HEK293 Cells, Humans, Immunoprecipitation, Phylogeny, Poly I-C, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Ubiquitin ligases play important roles in immune regulation. The human RNF114 (RING finger protein 114), an ubiquitin ligase, was recently reported to be involved in immune response to double-stranded RNA in disease pathogenesis. Here, we identified a RNF114 homolog in Chinese sturgeon (Acipenser sinensis) and investigated its potential role in immune response. The full-length cDNA of Chinese sturgeon RNF114 (csRNF114) contains an open reading frame (ORF) of 681 nucleotides coding a protein of 227 amino acids. csRNF114 shares the highest identity of 76% at amino acid level to other RNF114 homologs, clustering with bony fish RNF114s based on phylogenetic analysis. The main structural features of csRNF114, including a C3HC4 (Cys3-His-Cys4) RING domain, a C2HC (Cys2-His-Cys)-type zinc finger motif, a C2H2 (Cys2-His2)-type zinc finger motif, and a UIM (ubiquitin-interacting motif), take csRNF114 as an ubiquitin ligase. csRNF114 mRNA was widely expressed in various tissues and significantly up-regulated in poly(I:C)-treated Chinese sturgeon. Over-expression of csRNF114 in HEK293T cells significantly promoted both basal and poly(I:C)-induced activation of interferon regulatory transcription factor 3 (IRF3) and nuclear factor-κB (NF-κB) downstream retinoic acid inducible gene I (RIG-I) signaling pathway and expression of target genes type I interferon (IFN), which was nearly abolished by knockdown of RIG-I with specific human siRNA and by mutation of the C3HC4 RING domain (C28A/C31A) in csRNF114 as well. Furthermore, csRNF114 associated with ubiquitinated proteins in HEK293T cells, for which the C3HC4 RING domain was essential. These data suggested that an ubiquitin ligase RNF114 homolog with a potential role in antiviral response possibly through modulating RIG-I signaling pathway was cloned from Chinese sturgeon, which might contribute to our understanding of the immune biology of Chinese sturgeon.
DOI: 10.1016/j.fsi.2014.09.030
PubMed: 25290666
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pubmed:25290666Le document en format XML
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Electronic address: zyliap@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035</wicri:regionArea><wicri:noRegion>Wenzhou 325035</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xiaojun" sort="Chen, Xiaojun" uniqKey="Chen X" first="Xiaojun" last="Chen">Xiaojun Chen</name><affiliation wicri:level="1"><nlm:affiliation>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035</wicri:regionArea><wicri:noRegion>Wenzhou 325035</wicri:noRegion></affiliation></author><author><name sortKey="Nie, Dongsong" sort="Nie, Dongsong" uniqKey="Nie D" first="Dongsong" last="Nie">Dongsong Nie</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006</wicri:regionArea><wicri:noRegion>Yueyang 414006</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jiajia" sort="Wang, Jiajia" uniqKey="Wang J" first="Jiajia" last="Wang">Jiajia Wang</name><affiliation wicri:level="1"><nlm:affiliation>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035</wicri:regionArea><wicri:noRegion>Wenzhou 325035</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Mingjiang" sort="Wu, Mingjiang" uniqKey="Wu M" first="Mingjiang" last="Wu">Mingjiang Wu</name><affiliation wicri:level="1"><nlm:affiliation>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035</wicri:regionArea><wicri:noRegion>Wenzhou 325035</wicri:noRegion></affiliation></author></analytic><series><title level="j">Fish & shellfish immunology</title><idno type="eISSN">1095-9947</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Cloning, Molecular</term><term>Computational Biology</term><term>DNA Primers (genetics)</term><term>Fishes (genetics)</term><term>Fishes (immunology)</term><term>Gene Knockdown Techniques</term><term>Gene Library</term><term>HEK293 Cells</term><term>Humans</term><term>Immunoprecipitation</term><term>Interferons (metabolism)</term><term>NF-kappa B (metabolism)</term><term>Open Reading Frames (genetics)</term><term>Phylogeny</term><term>Poly I-C</term><term>RING Finger Domains (genetics)</term><term>RING Finger Domains (immunology)</term><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Signal Transduction (immunology)</term><term>Ubiquitin-Protein Ligases (genetics)</term><term>Ubiquitin-Protein Ligases (immunology)</term><term>Ubiquitin-Protein Ligases (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term><term>Open Reading Frames</term><term>RING Finger Domains</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Fishes</term><term>RING Finger Domains</term><term>Signal Transduction</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferons</term><term>NF-kappa B</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Cloning, Molecular</term><term>Computational Biology</term><term>Gene Knockdown Techniques</term><term>Gene Library</term><term>HEK293 Cells</term><term>Humans</term><term>Immunoprecipitation</term><term>Phylogeny</term><term>Poly I-C</term><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ubiquitin ligases play important roles in immune regulation. The human RNF114 (RING finger protein 114), an ubiquitin ligase, was recently reported to be involved in immune response to double-stranded RNA in disease pathogenesis. Here, we identified a RNF114 homolog in Chinese sturgeon (Acipenser sinensis) and investigated its potential role in immune response. The full-length cDNA of Chinese sturgeon RNF114 (csRNF114) contains an open reading frame (ORF) of 681 nucleotides coding a protein of 227 amino acids. csRNF114 shares the highest identity of 76% at amino acid level to other RNF114 homologs, clustering with bony fish RNF114s based on phylogenetic analysis. The main structural features of csRNF114, including a C3HC4 (Cys3-His-Cys4) RING domain, a C2HC (Cys2-His-Cys)-type zinc finger motif, a C2H2 (Cys2-His2)-type zinc finger motif, and a UIM (ubiquitin-interacting motif), take csRNF114 as an ubiquitin ligase. csRNF114 mRNA was widely expressed in various tissues and significantly up-regulated in poly(I:C)-treated Chinese sturgeon. Over-expression of csRNF114 in HEK293T cells significantly promoted both basal and poly(I:C)-induced activation of interferon regulatory transcription factor 3 (IRF3) and nuclear factor-κB (NF-κB) downstream retinoic acid inducible gene I (RIG-I) signaling pathway and expression of target genes type I interferon (IFN), which was nearly abolished by knockdown of RIG-I with specific human siRNA and by mutation of the C3HC4 RING domain (C28A/C31A) in csRNF114 as well. Furthermore, csRNF114 associated with ubiquitinated proteins in HEK293T cells, for which the C3HC4 RING domain was essential. These data suggested that an ubiquitin ligase RNF114 homolog with a potential role in antiviral response possibly through modulating RIG-I signaling pathway was cloned from Chinese sturgeon, which might contribute to our understanding of the immune biology of Chinese sturgeon.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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