Comparison of the sensitivity of four native Canadian fish species to 17-α ethinylestradiol, using an in vitro liver explant assay.
Identifieur interne : 000148 ( Main/Merge ); précédent : 000147; suivant : 000149Comparison of the sensitivity of four native Canadian fish species to 17-α ethinylestradiol, using an in vitro liver explant assay.
Auteurs : Shawn C. Beitel [Canada] ; Jon A. Doering [Canada] ; Bryanna K. Eisner [Canada] ; Markus Hecker [Canada]Source :
- Environmental science and pollution research international [ 1614-7499 ] ; 2015.
Descripteurs français
- Wicri :
- geographic : Canada.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Estrogen Receptor alpha, Estrogens, Vitellogenins.
- chemical , toxicity : Ethinyl Estradiol, Water Pollutants, Chemical.
- geographic : Canada.
- drug effects : Liver.
- metabolism : Fishes, Liver.
- Animals, Dose-Response Relationship, Drug, In Vitro Techniques, Male.
Abstract
Exposure to environmental estrogens and other endocrine-active chemicals can impact reproduction of freshwater fishes. While extensive data exists regarding the effect of estrogens on standard laboratory species, little is known about the sensitivity of freshwater fishes native to North America to these compounds. Current testing strategies for the toxicological assessment of contaminants still rely heavily on studies with live animals, which poses increasing concerns from an economical and ethical perspective. Therefore, the aim of the present study was to investigate the sensitivity of four native species, namely, northern pike (Esox lucius), walleye (Sander vitreus), white sucker (Catostomus commersoni), and juvenile white sturgeon (Acipenser transmontanus), to an environmental estrogen, 17α-ethinylestradiol (EE2), using an in vitro tissue explant approach. Transcript abundances of vitellogenin (VTG) as well as the estrogen receptors (ER) α and β were used as the measuring endpoints as they represent well established biomarkers previously used to assess exposure to estrogens. Transcript abundance of VTG was upregulated in a concentration-dependent manner in each species. Liver explants of male walleye were found to have the greatest sensitivity to EE2, with a lowest observable effect concentration of 300 ng/L (1.0 nM) for VTG transcript abundance, with juvenile white sturgeon having the greatest magnitude of VTG transcript upregulation in exposed tissue (15-fold relative to control). Exposure of liver explants to EE2 resulted in no alteration in transcript abundance of ERβ, whereas upregulation of ERα was observed in northern pike only. Based on in vitro expression of VTG, the species tested were among the species with greatest sensitivity to environmental estrogens tested to date.
DOI: 10.1007/s11356-015-5101-7
PubMed: 26304805
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Beitel</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Graduate Program, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada. scb839@mail.usask.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Graduate Program, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3</wicri:regionArea><wicri:noRegion>S7N 5B3</wicri:noRegion></affiliation></author><author><name sortKey="Doering, Jon A" sort="Doering, Jon A" uniqKey="Doering J" first="Jon A" last="Doering">Jon A. 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Eisner</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3</wicri:regionArea><wicri:noRegion>S7N 5B3</wicri:noRegion></affiliation></author><author><name sortKey="Hecker, Markus" sort="Hecker, Markus" uniqKey="Hecker M" first="Markus" last="Hecker">Markus Hecker</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada. markus.hecker@usask.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3</wicri:regionArea><wicri:noRegion>S7N 5B3</wicri:noRegion></affiliation></author></analytic><series><title level="j">Environmental science and pollution research international</title><idno type="eISSN">1614-7499</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Canada</term><term>Dose-Response Relationship, Drug</term><term>Estrogen Receptor alpha (metabolism)</term><term>Estrogens (metabolism)</term><term>Ethinyl Estradiol (toxicity)</term><term>Fishes (metabolism)</term><term>In Vitro Techniques</term><term>Liver (drug effects)</term><term>Liver (metabolism)</term><term>Male</term><term>Vitellogenins (metabolism)</term><term>Water Pollutants, Chemical (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Estrogen Receptor alpha</term><term>Estrogens</term><term>Vitellogenins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Ethinyl Estradiol</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Canada</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Liver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Dose-Response Relationship, Drug</term><term>In Vitro Techniques</term><term>Male</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Canada</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Exposure to environmental estrogens and other endocrine-active chemicals can impact reproduction of freshwater fishes. While extensive data exists regarding the effect of estrogens on standard laboratory species, little is known about the sensitivity of freshwater fishes native to North America to these compounds. Current testing strategies for the toxicological assessment of contaminants still rely heavily on studies with live animals, which poses increasing concerns from an economical and ethical perspective. Therefore, the aim of the present study was to investigate the sensitivity of four native species, namely, northern pike (Esox lucius), walleye (Sander vitreus), white sucker (Catostomus commersoni), and juvenile white sturgeon (Acipenser transmontanus), to an environmental estrogen, 17α-ethinylestradiol (EE2), using an in vitro tissue explant approach. Transcript abundances of vitellogenin (VTG) as well as the estrogen receptors (ER) α and β were used as the measuring endpoints as they represent well established biomarkers previously used to assess exposure to estrogens. Transcript abundance of VTG was upregulated in a concentration-dependent manner in each species. Liver explants of male walleye were found to have the greatest sensitivity to EE2, with a lowest observable effect concentration of 300 ng/L (1.0 nM) for VTG transcript abundance, with juvenile white sturgeon having the greatest magnitude of VTG transcript upregulation in exposed tissue (15-fold relative to control). Exposure of liver explants to EE2 resulted in no alteration in transcript abundance of ERβ, whereas upregulation of ERα was observed in northern pike only. Based on in vitro expression of VTG, the species tested were among the species with greatest sensitivity to environmental estrogens tested to date.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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