Functional C1q is present in the skin mucus of Siberian sturgeon (Acipenser baerii).
Identifieur interne : 000131 ( Main/Merge ); précédent : 000130; suivant : 000132Functional C1q is present in the skin mucus of Siberian sturgeon (Acipenser baerii).
Auteurs : Chunxin Fan [République populaire de Chine] ; Jian Wang ; Xuguang Zhang ; Jiakun SongSource :
- Integrative zoology [ 1749-4877 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Complement C1q.
- chemical , immunology : Complement C1q.
- drug effects : Escherichia coli.
- immunology : Fishes, Mucus, Skin.
- Animals, Immunity, Innate, Immunity, Mucosal, Phylogeny.
Abstract
The skin mucus of fish acts as the first line of self-protection against pathogens in the aquatic environment and comprises a number of innate immune components. However, the presence of the critical classical complement component C1q, which links the innate and adaptive immune systems of mammalians, has not been explored in a primitive actinopterygian fish. In this study, we report that C1q is present in the skin mucus of the Siberian sturgeon (Acipenser baerii). The skin mucus was able to inhibit the growth of Escherichia coli. The bacteriostatic activity of the skin mucus was reduced by heating and by pre-incubation with EDTA or mouse anti-human C1q antibody. We also detected C1q protein in skin mucus using the western blot procedure and isolated a cDNA that encodes the Siberian sturgeon C1qC, which had 44.7-51.4% identity with C1qCs in teleosts and tetrapods. A phylogenetic analysis revealed that Siberian sturgeon C1qC lies at the root of the actinopterygian branch and is separate from the tetrapod branch. The C1qC transcript was expressed in many tissues as well as in skin. Our data indicate that C1q is present in the skin mucus of the Siberian sturgeon to protect against water-borne bacteria, and the C1qC found in the sturgeon may represent the primitive form of teleost and tetrapod C1qCs.
DOI: 10.1111/1749-4877.12100
PubMed: 24920077
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pubmed:24920077Le document en format XML
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Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Marine Biosystem and Neuroscience, International Center for Marine Studies, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Zhang, Xuguang" sort="Zhang, Xuguang" uniqKey="Zhang X" first="Xuguang" last="Zhang">Xuguang Zhang</name></author><author><name sortKey="Song, Jiakun" sort="Song, Jiakun" uniqKey="Song J" first="Jiakun" last="Song">Jiakun Song</name></author></analytic><series><title level="j">Integrative zoology</title><idno type="eISSN">1749-4877</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Complement C1q (analysis)</term><term>Complement C1q (immunology)</term><term>Escherichia coli (drug effects)</term><term>Fishes (immunology)</term><term>Immunity, Innate</term><term>Immunity, Mucosal</term><term>Mucus (immunology)</term><term>Phylogeny</term><term>Skin (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Complement C1q</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Complement C1q</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Fishes</term><term>Mucus</term><term>Skin</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Immunity, Innate</term><term>Immunity, Mucosal</term><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The skin mucus of fish acts as the first line of self-protection against pathogens in the aquatic environment and comprises a number of innate immune components. However, the presence of the critical classical complement component C1q, which links the innate and adaptive immune systems of mammalians, has not been explored in a primitive actinopterygian fish. In this study, we report that C1q is present in the skin mucus of the Siberian sturgeon (Acipenser baerii). The skin mucus was able to inhibit the growth of Escherichia coli. The bacteriostatic activity of the skin mucus was reduced by heating and by pre-incubation with EDTA or mouse anti-human C1q antibody. We also detected C1q protein in skin mucus using the western blot procedure and isolated a cDNA that encodes the Siberian sturgeon C1qC, which had 44.7-51.4% identity with C1qCs in teleosts and tetrapods. A phylogenetic analysis revealed that Siberian sturgeon C1qC lies at the root of the actinopterygian branch and is separate from the tetrapod branch. The C1qC transcript was expressed in many tissues as well as in skin. Our data indicate that C1q is present in the skin mucus of the Siberian sturgeon to protect against water-borne bacteria, and the C1qC found in the sturgeon may represent the primitive form of teleost and tetrapod C1qCs.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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