Identification and response to metals of metallothionein in two ancient fishes: white sturgeon (Acipenser transmontanus) and lake sturgeon (Acipenser fulvescens).
Identifieur interne : 000128 ( Main/Merge ); précédent : 000127; suivant : 000129Identification and response to metals of metallothionein in two ancient fishes: white sturgeon (Acipenser transmontanus) and lake sturgeon (Acipenser fulvescens).
Auteurs : Jon A. Doering [Canada] ; Shawn C. Beitel [Canada] ; Bryanna K. Eisner [Canada] ; Timon Heide [Allemagne] ; Henner Hollert [Allemagne] ; John P. Giesy [République populaire de Chine] ; Markus Hecker [Canada] ; Steve B. Wiseman [Canada]Source :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [ 1532-0456 ] ; 2015.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Aquaculture, Base Sequence, British Columbia, Cadmium (toxicity), Copper (toxicity), Fish Proteins (chemistry), Fish Proteins (genetics), Fish Proteins (metabolism), Fishes (growth & development), Gene Expression Regulation, Developmental (drug effects), Liver (drug effects), Liver (growth & development), Liver (metabolism), Metallothionein (chemistry), Metallothionein (genetics), Metallothionein (metabolism), Metals, Heavy (toxicity), Molecular Sequence Data, Organ Culture Techniques (veterinary), Osmolar Concentration, Phylogeny, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), Sequence Alignment, Water Pollutants, Chemical (toxicity), Wisconsin, Zinc (toxicity).
- MESH :
- chemical , chemistry : Fish Proteins, Metallothionein, Recombinant Proteins.
- chemical , genetics : Fish Proteins, Metallothionein.
- chemical , metabolism : Fish Proteins, Metallothionein, Recombinant Proteins.
- chemical , toxicity : Cadmium, Copper, Metals, Heavy, Water Pollutants, Chemical, Zinc.
- geographic : British Columbia, Wisconsin.
- drug effects : Gene Expression Regulation, Developmental, Liver.
- growth & development : Fishes, Liver.
- metabolism : Liver.
- veterinary : Organ Culture Techniques.
- Amino Acid Sequence, Animals, Aquaculture, Base Sequence, Molecular Sequence Data, Osmolar Concentration, Phylogeny, Sequence Alignment.
Abstract
White sturgeon (Acipenser transmontanus) are among the most sensitive species of fishes to Cu, Cd, and Zn, but there is no information about sensitivity of lake sturgeon (Acipenser fulvescens). To begin to elucidate molecular mechanism(s) of sensitivity of sturgeons to metals a cDNA encoding metallothionein (MT) was amplified from livers of white sturgeon (WS-MT) and lake sturgeon (LS-MT), and expression in response to Cu, Cd, or Zn was characterized in liver explants from each species. The primary structure of WS-MT and LS-MT contained 20 cysteine residues, which is the same as MTs of teleost fishes. However, the primary structure of WS-MT and LS-MT contained 63 amino acids, which is longer than any MT identified in teleost fishes. Abundance of transcripts of WS-MT in explants exposed to 0.3, 3, 30, or 100 μg/L of Cu was 1.7-, 1.7-, 2.1-, and 2.6-fold less than in controls, respectively. In contrast, abundances of transcripts of WS-MT were 3.3- and 2.4-fold greater in explants exposed to 30 μg/L of Cd and 1000 μg/L of Zn, respectively. Abundance of transcripts of LS-MT was not significantly different at any concentration of Cu, Cd, or Zn. MT is hypothesized to represent a critical mechanism for detoxification of metals. Therefore, results of this study suggest that sensitivity of sturgeons to exposure to Cu, Cd, or Zn might be a result of the relatively lesser maximal response of MT to metals. The study also suggestslake sturgeon might be more sensitive than white sturgeon to metals.
DOI: 10.1016/j.cbpc.2015.03.002
PubMed: 25795035
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pubmed:25795035Le document en format XML
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Beitel</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Eisner, Bryanna K" sort="Eisner, Bryanna K" uniqKey="Eisner B" first="Bryanna K" last="Eisner">Bryanna K. 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Giesy</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK, Canada; Department of Biology and Chemistry, State Key Laboratory for Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China; School of Biological Science, University of Hong Kong, Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK, Canada; Department of Biology and Chemistry, State Key Laboratory for Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China; School of Biological Science, University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Hecker, Markus" sort="Hecker, Markus" uniqKey="Hecker M" first="Markus" last="Hecker">Markus Hecker</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK, Canada. Electronic address: markus.hecker@usask.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Wiseman, Steve B" sort="Wiseman, Steve B" uniqKey="Wiseman S" first="Steve B" last="Wiseman">Steve B. Wiseman</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25795035</idno><idno type="pmid">25795035</idno><idno type="doi">10.1016/j.cbpc.2015.03.002</idno><idno type="wicri:Area/PubMed/Corpus">000133</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000133</idno><idno type="wicri:Area/PubMed/Curation">000133</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000133</idno><idno type="wicri:Area/PubMed/Checkpoint">000133</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000133</idno><idno type="wicri:Area/Ncbi/Merge">000626</idno><idno type="wicri:Area/Ncbi/Curation">000626</idno><idno type="wicri:Area/Ncbi/Checkpoint">000626</idno><idno type="wicri:doubleKey">1532-0456:2015:Doering J:identification:and:response</idno><idno type="wicri:Area/Main/Merge">000128</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and response to metals of metallothionein in two ancient fishes: white sturgeon (Acipenser transmontanus) and lake sturgeon (Acipenser fulvescens).</title><author><name sortKey="Doering, Jon A" sort="Doering, Jon A" uniqKey="Doering J" first="Jon A" last="Doering">Jon A. Doering</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Beitel, Shawn C" sort="Beitel, Shawn C" uniqKey="Beitel S" first="Shawn C" last="Beitel">Shawn C. Beitel</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Eisner, Bryanna K" sort="Eisner, Bryanna K" uniqKey="Eisner B" first="Bryanna K" last="Eisner">Bryanna K. Eisner</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Heide, Timon" sort="Heide, Timon" uniqKey="Heide T" first="Timon" last="Heide">Timon Heide</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Environmental Research, RWTH University, Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Environmental Research, RWTH University, Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Hollert, Henner" sort="Hollert, Henner" uniqKey="Hollert H" first="Henner" last="Hollert">Henner Hollert</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Environmental Research, RWTH University, Aachen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Environmental Research, RWTH University, Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Giesy, John P" sort="Giesy, John P" uniqKey="Giesy J" first="John P" last="Giesy">John P. Giesy</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK, Canada; Department of Biology and Chemistry, State Key Laboratory for Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China; School of Biological Science, University of Hong Kong, Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK, Canada; Department of Biology and Chemistry, State Key Laboratory for Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China; School of Biological Science, University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Hecker, Markus" sort="Hecker, Markus" uniqKey="Hecker M" first="Markus" last="Hecker">Markus Hecker</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK, Canada. Electronic address: markus.hecker@usask.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada; School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Wiseman, Steve B" sort="Wiseman, Steve B" uniqKey="Wiseman S" first="Steve B" last="Wiseman">Steve B. Wiseman</name><affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author></analytic><series><title level="j">Comparative biochemistry and physiology. Toxicology & pharmacology : CBP</title><idno type="ISSN">1532-0456</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Aquaculture</term><term>Base Sequence</term><term>British Columbia</term><term>Cadmium (toxicity)</term><term>Copper (toxicity)</term><term>Fish Proteins (chemistry)</term><term>Fish Proteins (genetics)</term><term>Fish Proteins (metabolism)</term><term>Fishes (growth & development)</term><term>Gene Expression Regulation, Developmental (drug effects)</term><term>Liver (drug effects)</term><term>Liver (growth & development)</term><term>Liver (metabolism)</term><term>Metallothionein (chemistry)</term><term>Metallothionein (genetics)</term><term>Metallothionein (metabolism)</term><term>Metals, Heavy (toxicity)</term><term>Molecular Sequence Data</term><term>Organ Culture Techniques (veterinary)</term><term>Osmolar Concentration</term><term>Phylogeny</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (metabolism)</term><term>Sequence Alignment</term><term>Water Pollutants, Chemical (toxicity)</term><term>Wisconsin</term><term>Zinc (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fish Proteins</term><term>Metallothionein</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fish Proteins</term><term>Metallothionein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fish Proteins</term><term>Metallothionein</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term><term>Copper</term><term>Metals, Heavy</term><term>Water Pollutants, Chemical</term><term>Zinc</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>British Columbia</term><term>Wisconsin</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Developmental</term><term>Liver</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fishes</term><term>Liver</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Organ Culture Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Aquaculture</term><term>Base Sequence</term><term>Molecular Sequence Data</term><term>Osmolar Concentration</term><term>Phylogeny</term><term>Sequence Alignment</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">White sturgeon (Acipenser transmontanus) are among the most sensitive species of fishes to Cu, Cd, and Zn, but there is no information about sensitivity of lake sturgeon (Acipenser fulvescens). To begin to elucidate molecular mechanism(s) of sensitivity of sturgeons to metals a cDNA encoding metallothionein (MT) was amplified from livers of white sturgeon (WS-MT) and lake sturgeon (LS-MT), and expression in response to Cu, Cd, or Zn was characterized in liver explants from each species. The primary structure of WS-MT and LS-MT contained 20 cysteine residues, which is the same as MTs of teleost fishes. However, the primary structure of WS-MT and LS-MT contained 63 amino acids, which is longer than any MT identified in teleost fishes. Abundance of transcripts of WS-MT in explants exposed to 0.3, 3, 30, or 100 μg/L of Cu was 1.7-, 1.7-, 2.1-, and 2.6-fold less than in controls, respectively. In contrast, abundances of transcripts of WS-MT were 3.3- and 2.4-fold greater in explants exposed to 30 μg/L of Cd and 1000 μg/L of Zn, respectively. Abundance of transcripts of LS-MT was not significantly different at any concentration of Cu, Cd, or Zn. MT is hypothesized to represent a critical mechanism for detoxification of metals. Therefore, results of this study suggest that sensitivity of sturgeons to exposure to Cu, Cd, or Zn might be a result of the relatively lesser maximal response of MT to metals. The study also suggestslake sturgeon might be more sensitive than white sturgeon to metals.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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