Motility initiation of sterlet sturgeon (Acipenser ruthenus) spermatozoa: Describing the propagation of the first flagellar waves.
Identifieur interne : 000121 ( Main/Merge ); précédent : 000120; suivant : 000122Motility initiation of sterlet sturgeon (Acipenser ruthenus) spermatozoa: Describing the propagation of the first flagellar waves.
Auteurs : Galina Prokopchuk [République tchèque] ; Boris Dzyuba [République tchèque] ; Olga Bondarenko [République tchèque] ; Marek Rodina [République tchèque] ; Jacky Cosson [République tchèque]Source :
- Theriogenology [ 1879-3231 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- cytology : Spermatozoa.
- physiology : Fishes, Flagella, Spermatozoa.
- Animals, Aquaculture, Male, Sperm Motility.
Abstract
In the present study, for the first time in fish spermatozoa, we describe the precise chronology of motility initiation of sterlet (sturgeon) sperm from completely immotile flagella to regular full wave propagation. The successive activation steps were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds (10 ± 2.68 seconds). Starting from fully immotile, the flagellum shows some trembling for a brief period, soon followed by appearance of the first real bend (so-called "principal bend") with a large wave amplitude 4.28 ± 0.65 μm, then by the "reverse bend," the latter presenting a lower (P < 0.05) wave amplitude (1.14 ± 0.32 μm). This couple of first bends formed at the basal region begins to propagate toward the flagellar tip but gradually fades when reaching the midflagellum, wherein consequently the sperm cell remains nonprogressive. This behavior repeats several times until a stage where the amplitude of the reverse bend gradually reaches a value similar that of the principal bend: The larger amplitude of this couple of bends finally leads to sustain a real "takeoff" of the sperm cell characterized by a full flagellar wave propagation generating an active forward displacement similar to that occurring during regular steady state motility (several seconds after activation). Starting from the earliest stages of motility initiation, the wave propagation along the flagellum and formation of new waves proceeded in a helical manner leading to a 3-dimensional rotation of the whole spermatozoon. Eventually, we estimated that the time period needed from the activation signal (contact with fresh water) to full wave propagation ranges from 0.4 to 1.2 seconds.
DOI: 10.1016/j.theriogenology.2015.02.011
PubMed: 25794841
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000127
- to stream PubMed, to step Curation: 000127
- to stream PubMed, to step Checkpoint: 000127
- to stream Ncbi, to step Merge: 000625
- to stream Ncbi, to step Curation: 000625
- to stream Ncbi, to step Checkpoint: 000625
Links to Exploration step
pubmed:25794841Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Motility initiation of sterlet sturgeon (Acipenser ruthenus) spermatozoa: Describing the propagation of the first flagellar waves.</title>
<author><name sortKey="Prokopchuk, Galina" sort="Prokopchuk, Galina" uniqKey="Prokopchuk G" first="Galina" last="Prokopchuk">Galina Prokopchuk</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic. Electronic address: prokopchuk@frov.jcu.cz.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dzyuba, Boris" sort="Dzyuba, Boris" uniqKey="Dzyuba B" first="Boris" last="Dzyuba">Boris Dzyuba</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bondarenko, Olga" sort="Bondarenko, Olga" uniqKey="Bondarenko O" first="Olga" last="Bondarenko">Olga Bondarenko</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2015">2015</date>
<idno type="RBID">pubmed:25794841</idno>
<idno type="pmid">25794841</idno>
<idno type="doi">10.1016/j.theriogenology.2015.02.011</idno>
<idno type="wicri:Area/PubMed/Corpus">000127</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000127</idno>
<idno type="wicri:Area/PubMed/Curation">000127</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000127</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000127</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000127</idno>
<idno type="wicri:Area/Ncbi/Merge">000625</idno>
<idno type="wicri:Area/Ncbi/Curation">000625</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000625</idno>
<idno type="wicri:Area/Main/Merge">000121</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Motility initiation of sterlet sturgeon (Acipenser ruthenus) spermatozoa: Describing the propagation of the first flagellar waves.</title>
<author><name sortKey="Prokopchuk, Galina" sort="Prokopchuk, Galina" uniqKey="Prokopchuk G" first="Galina" last="Prokopchuk">Galina Prokopchuk</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic. Electronic address: prokopchuk@frov.jcu.cz.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dzyuba, Boris" sort="Dzyuba, Boris" uniqKey="Dzyuba B" first="Boris" last="Dzyuba">Boris Dzyuba</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bondarenko, Olga" sort="Bondarenko, Olga" uniqKey="Bondarenko O" first="Olga" last="Bondarenko">Olga Bondarenko</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name>
<affiliation wicri:level="1"><nlm:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation>
<country xml:lang="fr">République tchèque</country>
<wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea>
<wicri:noRegion>Vodňany</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Theriogenology</title>
<idno type="eISSN">1879-3231</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Aquaculture</term>
<term>Fishes (physiology)</term>
<term>Flagella (physiology)</term>
<term>Male</term>
<term>Sperm Motility</term>
<term>Spermatozoa (cytology)</term>
<term>Spermatozoa (physiology)</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spermatozoa</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term>
<term>Flagella</term>
<term>Spermatozoa</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Aquaculture</term>
<term>Male</term>
<term>Sperm Motility</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In the present study, for the first time in fish spermatozoa, we describe the precise chronology of motility initiation of sterlet (sturgeon) sperm from completely immotile flagella to regular full wave propagation. The successive activation steps were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds (10 ± 2.68 seconds). Starting from fully immotile, the flagellum shows some trembling for a brief period, soon followed by appearance of the first real bend (so-called "principal bend") with a large wave amplitude 4.28 ± 0.65 μm, then by the "reverse bend," the latter presenting a lower (P < 0.05) wave amplitude (1.14 ± 0.32 μm). This couple of first bends formed at the basal region begins to propagate toward the flagellar tip but gradually fades when reaching the midflagellum, wherein consequently the sperm cell remains nonprogressive. This behavior repeats several times until a stage where the amplitude of the reverse bend gradually reaches a value similar that of the principal bend: The larger amplitude of this couple of bends finally leads to sustain a real "takeoff" of the sperm cell characterized by a full flagellar wave propagation generating an active forward displacement similar to that occurring during regular steady state motility (several seconds after activation). Starting from the earliest stages of motility initiation, the wave propagation along the flagellum and formation of new waves proceeded in a helical manner leading to a 3-dimensional rotation of the whole spermatozoon. Eventually, we estimated that the time period needed from the activation signal (contact with fresh water) to full wave propagation ranges from 0.4 to 1.2 seconds.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000121 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000121 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:25794841 |texte= Motility initiation of sterlet sturgeon (Acipenser ruthenus) spermatozoa: Describing the propagation of the first flagellar waves. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:25794841" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |