The influence of cryoprotectants on sturgeon (Acipenser ruthenus) sperm quality, DNA integrity, antioxidant responses, and resistance to oxidative stress.
Identifieur interne : 000106 ( Main/Merge ); précédent : 000105; suivant : 000107The influence of cryoprotectants on sturgeon (Acipenser ruthenus) sperm quality, DNA integrity, antioxidant responses, and resistance to oxidative stress.
Auteurs : Anna Shaliutina-Kolešová [République tchèque] ; Jacky Cosson [République tchèque] ; Ievgen Lebeda [République tchèque] ; Ievgenia Gazo [République tchèque] ; Olena Shaliutina [République tchèque] ; Boris Dzyuba [République tchèque] ; Otomar Linhart [République tchèque]Source :
- Animal reproduction science [ 1873-2232 ] ; 2015.
English descriptors
- KwdEn :
- Acetamides (pharmacology), Animals, Cryopreservation (veterinary), Cryoprotective Agents (pharmacology), DNA (drug effects), DNA Fragmentation (drug effects), Dimethyl Sulfoxide (pharmacology), Ethylene Glycol (pharmacology), Fishes (physiology), Male, Methanol (pharmacology), Oxidation-Reduction (drug effects), Oxidative Stress (drug effects), Spermatozoa (drug effects).
- MESH :
- chemical , drug effects : DNA.
- chemical , pharmacology : Acetamides, Cryoprotective Agents, Dimethyl Sulfoxide, Ethylene Glycol, Methanol.
- drug effects : DNA Fragmentation, Oxidation-Reduction, Oxidative Stress, Spermatozoa.
- physiology : Fishes.
- veterinary : Cryopreservation.
- Animals, Male.
Abstract
This study examined the effect of cryoprotectants on DNA integrity, antioxidant defense, and resistance to oxidative stress in cryopreserved sterlet Acipenser ruthenus sperm. The freeze-thaw process significantly influenced sperm motility, with significant differences among cryoprotectants. In vitro exposure of cryopreserved sperm to the xanthine-xanthine oxidase (X-XO) system as a model reactive oxygen species inducer resulted in a lesser motility rate and velocity compared to the control, and there was a decrease in these variables in a time- and dose-dependent manner. The greatest X (0.6mM)-XO (0.05U/mL) concentration and incubation period (30min) was associated with 62% DNA fragmentation in sperm cryopreserved with 10% ethylene glycol (EG). The maximum lipid peroxidation (LPO) and carbonyl derivatives of proteins (CP) was also observed in sperm cryopreserved with 10% EG and exposed to the X-XO system at a concentration of 0.6mM X-0.05U/mL XO. The frozen/thawed sperm containing 10% EG and that with 10% dimethyl sulfoxide (DMSO) had a significant enhancement of superoxide dismutase (SOD) and glutathione reductase (GR) activity. The current study confirms that EG is not effective for cryopreservation, and sterlet sperm were highly sensitive to free radicals after cryopreservation with EG.
DOI: 10.1016/j.anireprosci.2015.05.014
PubMed: 26089085
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pubmed:26089085Le document en format XML
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tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Lebeda, Ievgen" sort="Lebeda, Ievgen" uniqKey="Lebeda I" first="Ievgen" last="Lebeda">Ievgen Lebeda</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and 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sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno 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stress.</title><author><name sortKey="Shaliutina Kolesova, Anna" sort="Shaliutina Kolesova, Anna" uniqKey="Shaliutina Kolesova A" first="Anna" last="Shaliutina-Kolešová">Anna Shaliutina-Kolešová</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: shalia00@frov.jcu.cz.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Lebeda, Ievgen" sort="Lebeda, Ievgen" uniqKey="Lebeda I" first="Ievgen" last="Lebeda">Ievgen Lebeda</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Gazo, Ievgenia" sort="Gazo, Ievgenia" uniqKey="Gazo I" first="Ievgenia" last="Gazo">Ievgenia Gazo</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Shaliutina, Olena" sort="Shaliutina, Olena" uniqKey="Shaliutina O" first="Olena" last="Shaliutina">Olena Shaliutina</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Dzyuba, Boris" sort="Dzyuba, Boris" uniqKey="Dzyuba B" first="Boris" last="Dzyuba">Boris Dzyuba</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author></analytic><series><title level="j">Animal reproduction science</title><idno type="eISSN">1873-2232</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetamides (pharmacology)</term><term>Animals</term><term>Cryopreservation (veterinary)</term><term>Cryoprotective Agents (pharmacology)</term><term>DNA (drug effects)</term><term>DNA Fragmentation (drug effects)</term><term>Dimethyl Sulfoxide (pharmacology)</term><term>Ethylene Glycol (pharmacology)</term><term>Fishes (physiology)</term><term>Male</term><term>Methanol (pharmacology)</term><term>Oxidation-Reduction (drug effects)</term><term>Oxidative Stress (drug effects)</term><term>Spermatozoa (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetamides</term><term>Cryoprotective Agents</term><term>Dimethyl Sulfoxide</term><term>Ethylene Glycol</term><term>Methanol</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>DNA Fragmentation</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Cryopreservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study examined the effect of cryoprotectants on DNA integrity, antioxidant defense, and resistance to oxidative stress in cryopreserved sterlet Acipenser ruthenus sperm. The freeze-thaw process significantly influenced sperm motility, with significant differences among cryoprotectants. In vitro exposure of cryopreserved sperm to the xanthine-xanthine oxidase (X-XO) system as a model reactive oxygen species inducer resulted in a lesser motility rate and velocity compared to the control, and there was a decrease in these variables in a time- and dose-dependent manner. The greatest X (0.6mM)-XO (0.05U/mL) concentration and incubation period (30min) was associated with 62% DNA fragmentation in sperm cryopreserved with 10% ethylene glycol (EG). The maximum lipid peroxidation (LPO) and carbonyl derivatives of proteins (CP) was also observed in sperm cryopreserved with 10% EG and exposed to the X-XO system at a concentration of 0.6mM X-0.05U/mL XO. The frozen/thawed sperm containing 10% EG and that with 10% dimethyl sulfoxide (DMSO) had a significant enhancement of superoxide dismutase (SOD) and glutathione reductase (GR) activity. The current study confirms that EG is not effective for cryopreservation, and sterlet sperm were highly sensitive to free radicals after cryopreservation with EG.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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