Conservation, sex-biased expression and functional annotation of microRNAs in the gonad of Amur sturgeon (Acipenser schrenckii).
Identifieur interne : 000088 ( Main/Merge ); précédent : 000087; suivant : 000089Conservation, sex-biased expression and functional annotation of microRNAs in the gonad of Amur sturgeon (Acipenser schrenckii).
Auteurs : Xiujuan Zhang [République populaire de Chine] ; Lihong Yuan [République populaire de Chine] ; Linmiao Li [République populaire de Chine] ; Haiying Jiang [République populaire de Chine] ; Jinping Chen [République populaire de Chine]Source :
- Comparative biochemistry and physiology. Part D, Genomics & proteomics [ 1878-0407 ] ; 2016.
Abstract
MicroRNAs (miRNAs) are involved in post-transcriptional gene regulation and have crucial roles in regulating the expression of gametogenesis-related genes in animals. However, the mechanism of sex determination and differentiation in the sturgeon has remained unclear. Identifying miRNAs and characterizing sex-biased miRNA expression is therefore critical for understanding the role of miRNAs during sexual differentiation in sturgeon. In this study, five different tissues from sturgeon before sex differentiation and the gonads were used for miRNA expression profiling. We screened 1037 miRNAs in miRBase 20.0 and an additional 103 sturgeon miRNAs using microarray and real-time PCR. We found that the sequences of 477 miRNAs out of a total of 1140 miRNAs were highly conserved (100%) among different fish species. From a total of 663 non-redundant miRNA probes, 481 miRNAs were detected in the gonads of both sexes. Of the 148 miRNAs that were identified to have sex-biased expression patterns between the testis and the ovary (P<0.01), 21 miRNAs (14.19%) were relatively highly expressed in the testis or the ovary with fold-changes >2. The microarray expression patterns of 13 randomly selected sex-biased miRNAs were validated using real-time PCR. Target gene prediction revealed a significant enrichment of functional groups (88 GO terms) and 18 KEGG pathways (P<0.05) and suggested that there are interactions between sex-biased miRNAs and 25 putative gametogenesis-related targets. Therefore, our miRNA expression analysis in juvenile A. schrenckii establishes a foundation for understanding and further investigating the role of miRNAs in sturgeon sex differentiation.
DOI: 10.1016/j.cbd.2016.04.001
PubMed: 27089517
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Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou, China; Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Entomological Institute/South China Institute of Endangered Animals, Guangzhou, China; Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou, China; Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yuan, Lihong" sort="Yuan, Lihong" uniqKey="Yuan L" first="Lihong" last="Yuan">Lihong Yuan</name><affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute/South China Institute of Endangered Animals, Guangzhou, China; Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou, China; Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Entomological Institute/South China Institute of Endangered Animals, Guangzhou, China; Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou, China; Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Linmiao" sort="Li, Linmiao" uniqKey="Li L" first="Linmiao" last="Li">Linmiao Li</name><affiliation wicri:level="3"><nlm:affiliation>Guangdong Entomological Institute/South China Institute of Endangered Animals, Guangzhou, China; Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou, China; Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Entomological Institute/South China Institute of Endangered Animals, Guangzhou, China; 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Part D, Genomics & proteomics</title><idno type="eISSN">1878-0407</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">MicroRNAs (miRNAs) are involved in post-transcriptional gene regulation and have crucial roles in regulating the expression of gametogenesis-related genes in animals. However, the mechanism of sex determination and differentiation in the sturgeon has remained unclear. Identifying miRNAs and characterizing sex-biased miRNA expression is therefore critical for understanding the role of miRNAs during sexual differentiation in sturgeon. In this study, five different tissues from sturgeon before sex differentiation and the gonads were used for miRNA expression profiling. We screened 1037 miRNAs in miRBase 20.0 and an additional 103 sturgeon miRNAs using microarray and real-time PCR. We found that the sequences of 477 miRNAs out of a total of 1140 miRNAs were highly conserved (100%) among different fish species. From a total of 663 non-redundant miRNA probes, 481 miRNAs were detected in the gonads of both sexes. Of the 148 miRNAs that were identified to have sex-biased expression patterns between the testis and the ovary (P<0.01), 21 miRNAs (14.19%) were relatively highly expressed in the testis or the ovary with fold-changes >2. The microarray expression patterns of 13 randomly selected sex-biased miRNAs were validated using real-time PCR. Target gene prediction revealed a significant enrichment of functional groups (88 GO terms) and 18 KEGG pathways (P<0.05) and suggested that there are interactions between sex-biased miRNAs and 25 putative gametogenesis-related targets. Therefore, our miRNA expression analysis in juvenile A. schrenckii establishes a foundation for understanding and further investigating the role of miRNAs in sturgeon sex differentiation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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