Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region.
Identifieur interne : 000059 ( Main/Merge ); précédent : 000058; suivant : 000060Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region.
Auteurs : Huan Ye [République populaire de Chine] ; Hua-Mei Yue [République populaire de Chine] ; Xiao-Ge Yang [République populaire de Chine] ; Chuang-Ju Li [République populaire de Chine] ; Qi-Wei Wei [République populaire de Chine]Source :
- Cell and tissue research [ 1432-0878 ] ; 2016.
Abstract
Germ cells are set aside from somatic cells early in embryogenesis, and are responsible for transmitting genetic information through generations. Vasa is a highly conserved germ cell marker across animal phyla, and widely used to label primordial germ cells. Dabry's sturgeon is a rare and endangered species distributed solely in the Yangtze River basin. Here, seven vasa isoforms, named Advasa1-7, were isolated and characterized in Dabry's sturgeon. RT-PCR and western blot analyses revealed that vasa mRNA and protein were mainly restricted to the testis and ovary, but exhibited sexually dimorphic expression. Cellular and subcellular localization uncovered that Advasa mRNA and protein displayed mitotic and meiotic expression in females, and mainly showed mitotic expression in males; surprisingly, they exhibited both cytoplasmic and nuclear expression in the ovarian germ cells, while showing exclusively cytoplasmic expression in the testicular germ cells. By microinjecting chimeric RNA consisting of the red fluorescent protein coding region and the Advasa 3'-untranslated region into embryos of Dabry's sturgeon, zebrafish and medaka, we demonstrated that it had the ability to visualize primordial germ cells (PGCs) in Dabry's sturgeon and zebrafish but not in medaka. It seemed that the machinery of vasa 3'UTR RNA localization was conserved between Dabry's sturgeon and ostariophysan, while possibly changed during the divergence of euteleosts and ostariophysan. Finally, Dabry's sturgeon PGCs moved on the yolk ball, and migrated toward the genital ridge via mesenchyme. Taken together, these results provide new information for vasa expression pattern and function, and lay a foundation for PGC cryopreservation and conservation of Dabry's sturgeon.
DOI: 10.1007/s00441-016-2418-6
PubMed: 27184950
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000033
- to stream PubMed, to step Curation: 000033
- to stream PubMed, to step Checkpoint: 000033
- to stream Ncbi, to step Merge: 000700
- to stream Ncbi, to step Curation: 000700
- to stream Ncbi, to step Checkpoint: 000700
Links to Exploration step
pubmed:27184950Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region.</title><author><name sortKey="Ye, Huan" sort="Ye, Huan" uniqKey="Ye H" first="Huan" last="Ye">Huan Ye</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072</wicri:regionArea><wicri:noRegion>430072</wicri:noRegion></affiliation></author><author><name sortKey="Yue, Hua Mei" sort="Yue, Hua Mei" uniqKey="Yue H" first="Hua-Mei" last="Yue">Hua-Mei Yue</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223</wicri:regionArea><wicri:noRegion>430223</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiao Ge" sort="Yang, Xiao Ge" uniqKey="Yang X" first="Xiao-Ge" last="Yang">Xiao-Ge Yang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072</wicri:regionArea><wicri:noRegion>430072</wicri:noRegion></affiliation></author><author><name sortKey="Li, Chuang Ju" sort="Li, Chuang Ju" uniqKey="Li C" first="Chuang-Ju" last="Li">Chuang-Ju Li</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China. lcj@yfi.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223</wicri:regionArea><wicri:noRegion>430223</wicri:noRegion></affiliation></author><author><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China. weiqw@yfi.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072</wicri:regionArea><wicri:noRegion>430072</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27184950</idno><idno type="pmid">27184950</idno><idno type="doi">10.1007/s00441-016-2418-6</idno><idno type="wicri:Area/PubMed/Corpus">000033</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000033</idno><idno type="wicri:Area/PubMed/Curation">000033</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000033</idno><idno type="wicri:Area/PubMed/Checkpoint">000033</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000033</idno><idno type="wicri:Area/Ncbi/Merge">000700</idno><idno type="wicri:Area/Ncbi/Curation">000700</idno><idno type="wicri:Area/Ncbi/Checkpoint">000700</idno><idno type="wicri:Area/Main/Merge">000059</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region.</title><author><name sortKey="Ye, Huan" sort="Ye, Huan" uniqKey="Ye H" first="Huan" last="Ye">Huan Ye</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072</wicri:regionArea><wicri:noRegion>430072</wicri:noRegion></affiliation></author><author><name sortKey="Yue, Hua Mei" sort="Yue, Hua Mei" uniqKey="Yue H" first="Hua-Mei" last="Yue">Hua-Mei Yue</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223</wicri:regionArea><wicri:noRegion>430223</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiao Ge" sort="Yang, Xiao Ge" uniqKey="Yang X" first="Xiao-Ge" last="Yang">Xiao-Ge Yang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072</wicri:regionArea><wicri:noRegion>430072</wicri:noRegion></affiliation></author><author><name sortKey="Li, Chuang Ju" sort="Li, Chuang Ju" uniqKey="Li C" first="Chuang-Ju" last="Li">Chuang-Ju Li</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China. lcj@yfi.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223</wicri:regionArea><wicri:noRegion>430223</wicri:noRegion></affiliation></author><author><name sortKey="Wei, Qi Wei" sort="Wei, Qi Wei" uniqKey="Wei Q" first="Qi-Wei" last="Wei">Qi-Wei Wei</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China. weiqw@yfi.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072</wicri:regionArea><wicri:noRegion>430072</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cell and tissue research</title><idno type="eISSN">1432-0878</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Germ cells are set aside from somatic cells early in embryogenesis, and are responsible for transmitting genetic information through generations. Vasa is a highly conserved germ cell marker across animal phyla, and widely used to label primordial germ cells. Dabry's sturgeon is a rare and endangered species distributed solely in the Yangtze River basin. Here, seven vasa isoforms, named Advasa1-7, were isolated and characterized in Dabry's sturgeon. RT-PCR and western blot analyses revealed that vasa mRNA and protein were mainly restricted to the testis and ovary, but exhibited sexually dimorphic expression. Cellular and subcellular localization uncovered that Advasa mRNA and protein displayed mitotic and meiotic expression in females, and mainly showed mitotic expression in males; surprisingly, they exhibited both cytoplasmic and nuclear expression in the ovarian germ cells, while showing exclusively cytoplasmic expression in the testicular germ cells. By microinjecting chimeric RNA consisting of the red fluorescent protein coding region and the Advasa 3'-untranslated region into embryos of Dabry's sturgeon, zebrafish and medaka, we demonstrated that it had the ability to visualize primordial germ cells (PGCs) in Dabry's sturgeon and zebrafish but not in medaka. It seemed that the machinery of vasa 3'UTR RNA localization was conserved between Dabry's sturgeon and ostariophysan, while possibly changed during the divergence of euteleosts and ostariophysan. Finally, Dabry's sturgeon PGCs moved on the yolk ball, and migrated toward the genital ridge via mesenchyme. Taken together, these results provide new information for vasa expression pattern and function, and lay a foundation for PGC cryopreservation and conservation of Dabry's sturgeon.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000059 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000059 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:27184950
|texte= Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:27184950" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |