Determination of cortisol in lake sturgeon (Acipenser fulvescens) eggs by liquid chromatography tandem mass spectrometry.
Identifieur interne : 000013 ( Main/Merge ); précédent : 000012; suivant : 000014Determination of cortisol in lake sturgeon (Acipenser fulvescens) eggs by liquid chromatography tandem mass spectrometry.
Auteurs : Ugo Bussy [États-Unis] ; Lydia Wassink [États-Unis] ; Kim T. Scribner [États-Unis] ; Weiming Li [États-Unis]Source :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1873-376X ] ; 2017.
Abstract
Quantifying cortisol concentrations in fish eggs is important to understand the effects of environmental conditions on maternal physiological condition and on egg provisioning and quality. Data are particularly relevant to studies of the ecology of threatened species such as lake sturgeon (Aciperser fulvescens) as well as assessments of larval physical and behavioral phenotypes, fish health and caviar quality in sturgeon aquaculture. This study focuses on development of bioanalytical methods for high sensitivity and robust determination of cortisol in sturgeon eggs. Sample preparation was optimized after investigating protein precipitation and liquid-liquid extraction techniques. Ethyl acetate was found to be the most efficient solvent (recovery parameter) and also provided the best sample clean up (matrix effect parameter). The method was determined to be linear for cortisol concentrations between 0.025 and 100ng/mL. The limits of detection and quantification were 0.025 and 0.1ng/mL respectively. Intra- and inter-day performances of the method were validated at three concentrations (0.25; 10 and 100ng/mL). The method was applied to field-collected samples for the determination of endogenous cortisol in lake sturgeon eggs. Cortisol was detected in all egg samples and statistical analysis showed significant differences between fertilized and non-fertilized eggs.
DOI: 10.1016/j.jchromb.2016.11.028
PubMed: 27984758
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Scribner</name><affiliation wicri:level="4"><nlm:affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA; Department of Integrative Biology, Michigan State University, East Lansing, MI 48824, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA; Department of Integrative Biology, Michigan State University, East Lansing, MI 48824</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author><author><name sortKey="Li, Weiming" sort="Li, Weiming" uniqKey="Li W" first="Weiming" last="Li">Weiming Li</name><affiliation wicri:level="4"><nlm:affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA; Department of Physiology, Michigan State University, East Lansing, MI 48824, USA. Electronic address: liweim@msu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA; Department of Physiology, Michigan State University, East Lansing, MI 48824</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="eISSN">1873-376X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Quantifying cortisol concentrations in fish eggs is important to understand the effects of environmental conditions on maternal physiological condition and on egg provisioning and quality. Data are particularly relevant to studies of the ecology of threatened species such as lake sturgeon (Aciperser fulvescens) as well as assessments of larval physical and behavioral phenotypes, fish health and caviar quality in sturgeon aquaculture. This study focuses on development of bioanalytical methods for high sensitivity and robust determination of cortisol in sturgeon eggs. Sample preparation was optimized after investigating protein precipitation and liquid-liquid extraction techniques. Ethyl acetate was found to be the most efficient solvent (recovery parameter) and also provided the best sample clean up (matrix effect parameter). The method was determined to be linear for cortisol concentrations between 0.025 and 100ng/mL. The limits of detection and quantification were 0.025 and 0.1ng/mL respectively. Intra- and inter-day performances of the method were validated at three concentrations (0.25; 10 and 100ng/mL). The method was applied to field-collected samples for the determination of endogenous cortisol in lake sturgeon eggs. Cortisol was detected in all egg samples and statistical analysis showed significant differences between fertilized and non-fertilized eggs.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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