Molecular identification of iridoviruses infecting various sturgeon species in Europe.
Identifieur interne : 000006 ( Main/Exploration ); précédent : 000005; suivant : 000007Molecular identification of iridoviruses infecting various sturgeon species in Europe.
Auteurs : L. Bigarré [France] ; M. Lesne [France] ; A. Lautraite [France] ; V. Chesneau [France] ; A. Leroux [France] ; M. Jamin [France] ; P M Boitard [France] ; A. Toffan [Italie] ; M. Prearo [Italie] ; S. Labrut [France] ; P. Daniel [France]Source :
- Journal of fish diseases [ 1365-2761 ] ; 2017.
Abstract
Iridoviridae are known to cause disease in sturgeons in North America. Here, histological and molecular methods were used to screen for this family of virus in sturgeons from various European farms with low-to-high morbidity. Some histological samples revealed basophilic cells in the gill and labial epithelia, strongly suggesting the accumulation of iridovirus particles. Newly developed generic PCR tests targeting the major capsid protein (MCP) gene of sturgeon iridoviruses identified in North America, namely the white sturgeon iridovirus and the Namao virus (NV), produced positive signals in most samples from four sturgeon species: Russian (Acipenser gueldenstaedtii), Siberian (A. baerii), Adriatic (A. naccarii) and beluga (Huso huso). The sequences of the PCR products were generally highly similar one another, with nucleotide identities greater than 98%. They were also related to (74-88%), although distinct from, American sturgeon iridoviruses. These European viruses were thus considered variants of a single new virus, provisionally named Acipenser iridovirus-European (AcIV-E). Moreover, three samples infected with AcIV-E showed genetic heterogeneity, with the co-existence of two sequences differing by five nucleotides. One of our European samples carried a virus distinct from AcIV-E, but closely related to NV identified in Canada (95%). This study demonstrates the presence of two distinct sturgeon iridoviruses in Europe: a new genotype AcIV-E and an NV-related virus.
DOI: 10.1111/jfd.12498
PubMed: 27193445
Affiliations:
- France, Italie
- Aquitaine, Nouvelle-Aquitaine, Pays de la Loire, Piémont
- Mont-de-Marsan, Nantes, Turin
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Bigarré</name><affiliation wicri:level="1"><nlm:affiliation>ANSES, Laboratoire de Ploufragan-Plouzané, Université Bretagne-Loire, Plouzané, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Laboratoire de Ploufragan-Plouzané, Université Bretagne-Loire, Plouzané</wicri:regionArea><wicri:noRegion>Plouzané</wicri:noRegion><wicri:noRegion>Plouzané</wicri:noRegion></affiliation></author><author><name sortKey="Lesne, M" sort="Lesne, M" uniqKey="Lesne M" first="M" last="Lesne">M. 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Jamin</name><affiliation wicri:level="1"><nlm:affiliation>Fili@vet, Aquaculture veterinarians, Saint-Martin-des-Champs, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Fili@vet, Aquaculture veterinarians, Saint-Martin-des-Champs</wicri:regionArea><wicri:noRegion>Saint-Martin-des-Champs</wicri:noRegion><wicri:noRegion>Saint-Martin-des-Champs</wicri:noRegion></affiliation></author><author><name sortKey="Boitard, P M" sort="Boitard, P M" uniqKey="Boitard P" first="P M" last="Boitard">P M Boitard</name><affiliation wicri:level="1"><nlm:affiliation>Fili@vet, Aquaculture veterinarians, Saint-Martin-des-Champs, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Fili@vet, Aquaculture veterinarians, Saint-Martin-des-Champs</wicri:regionArea><wicri:noRegion>Saint-Martin-des-Champs</wicri:noRegion><wicri:noRegion>Saint-Martin-des-Champs</wicri:noRegion></affiliation></author><author><name sortKey="Toffan, A" sort="Toffan, A" uniqKey="Toffan A" first="A" last="Toffan">A. Toffan</name><affiliation wicri:level="1"><nlm:affiliation>IZSVe, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>IZSVe, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro</wicri:regionArea><wicri:noRegion>Legnaro</wicri:noRegion></affiliation></author><author><name sortKey="Prearo, M" sort="Prearo, M" uniqKey="Prearo M" first="M" last="Prearo">M. Prearo</name><affiliation wicri:level="3"><nlm:affiliation>IZS PLV, Istituto Zooprofilattico Sperimentale del Piemonte, Torino, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>IZS PLV, Istituto Zooprofilattico Sperimentale del Piemonte, Torino</wicri:regionArea><placeName><settlement type="city">Turin</settlement><region type="région" nuts="2">Piémont</region></placeName></affiliation></author><author><name sortKey="Labrut, S" sort="Labrut, S" uniqKey="Labrut S" first="S" last="Labrut">S. Labrut</name><affiliation wicri:level="3"><nlm:affiliation>ONIRIS, AMaROC, LUNAM University, Nantes, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ONIRIS, AMaROC, LUNAM University, Nantes</wicri:regionArea><placeName><region type="region">Pays de la Loire</region><region type="old region">Pays de la Loire</region><settlement type="city">Nantes</settlement></placeName></affiliation></author><author><name sortKey="Daniel, P" sort="Daniel, P" uniqKey="Daniel P" first="P" last="Daniel">P. Daniel</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire des Pyrénées et des Landes, Mont-de-Marsan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Pyrénées et des Landes, Mont-de-Marsan</wicri:regionArea><placeName><region type="region">Nouvelle-Aquitaine</region><region type="old region">Aquitaine</region><settlement type="city">Mont-de-Marsan</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of fish diseases</title><idno type="eISSN">1365-2761</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Iridoviridae are known to cause disease in sturgeons in North America. Here, histological and molecular methods were used to screen for this family of virus in sturgeons from various European farms with low-to-high morbidity. Some histological samples revealed basophilic cells in the gill and labial epithelia, strongly suggesting the accumulation of iridovirus particles. Newly developed generic PCR tests targeting the major capsid protein (MCP) gene of sturgeon iridoviruses identified in North America, namely the white sturgeon iridovirus and the Namao virus (NV), produced positive signals in most samples from four sturgeon species: Russian (Acipenser gueldenstaedtii), Siberian (A. baerii), Adriatic (A. naccarii) and beluga (Huso huso). The sequences of the PCR products were generally highly similar one another, with nucleotide identities greater than 98%. They were also related to (74-88%), although distinct from, American sturgeon iridoviruses. These European viruses were thus considered variants of a single new virus, provisionally named Acipenser iridovirus-European (AcIV-E). Moreover, three samples infected with AcIV-E showed genetic heterogeneity, with the co-existence of two sequences differing by five nucleotides. One of our European samples carried a virus distinct from AcIV-E, but closely related to NV identified in Canada (95%). This study demonstrates the presence of two distinct sturgeon iridoviruses in Europe: a new genotype AcIV-E and an NV-related virus.</div></front></TEI><affiliations><list><country><li>France</li><li>Italie</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li><li>Pays de la Loire</li><li>Piémont</li></region><settlement><li>Mont-de-Marsan</li><li>Nantes</li><li>Turin</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Bigarre, L" sort="Bigarre, L" uniqKey="Bigarre L" first="L" last="Bigarré">L. Bigarré</name></noRegion><name sortKey="Boitard, P M" sort="Boitard, P M" uniqKey="Boitard P" first="P M" last="Boitard">P M Boitard</name><name sortKey="Chesneau, V" sort="Chesneau, V" uniqKey="Chesneau V" first="V" last="Chesneau">V. Chesneau</name><name sortKey="Daniel, P" sort="Daniel, P" uniqKey="Daniel P" first="P" last="Daniel">P. Daniel</name><name sortKey="Jamin, M" sort="Jamin, M" uniqKey="Jamin M" first="M" last="Jamin">M. Jamin</name><name sortKey="Labrut, S" sort="Labrut, S" uniqKey="Labrut S" first="S" last="Labrut">S. Labrut</name><name sortKey="Lautraite, A" sort="Lautraite, A" uniqKey="Lautraite A" first="A" last="Lautraite">A. Lautraite</name><name sortKey="Leroux, A" sort="Leroux, A" uniqKey="Leroux A" first="A" last="Leroux">A. Leroux</name><name sortKey="Lesne, M" sort="Lesne, M" uniqKey="Lesne M" first="M" last="Lesne">M. Lesne</name></country><country name="Italie"><noRegion><name sortKey="Toffan, A" sort="Toffan, A" uniqKey="Toffan A" first="A" last="Toffan">A. Toffan</name></noRegion><name sortKey="Prearo, M" sort="Prearo, M" uniqKey="Prearo M" first="M" last="Prearo">M. Prearo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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