Neomycin damage and regeneration of hair cells in both mechanoreceptor and electroreceptor lateral line organs of the larval Siberian sturgeon (Acipenser baerii).
Identifieur interne : 000048 ( Main/Exploration ); précédent : 000047; suivant : 000049Neomycin damage and regeneration of hair cells in both mechanoreceptor and electroreceptor lateral line organs of the larval Siberian sturgeon (Acipenser baerii).
Auteurs : Chunxin Fan [République populaire de Chine] ; Sha Zou [République populaire de Chine] ; Jian Wang [République populaire de Chine] ; Bo Zhang [République populaire de Chine] ; Jiakun Song [République populaire de Chine]Source :
- The Journal of comparative neurology [ 1096-9861 ] ; 2016.
English descriptors
- KwdEn :
- Age Factors, Animals, Cell Proliferation (drug effects), Dose-Response Relationship, Drug, Embryo, Nonmammalian, Fishes, Gene Expression Regulation, Developmental (drug effects), Gentamicins (metabolism), Hair Cells, Auditory (drug effects), Hair Cells, Auditory (ultrastructure), Larva (anatomy & histology), Lateral Line System (cytology), Mechanoreceptors (cytology), Microscopy, Electron, Scanning, Neomycin (toxicity), Nuclear Proteins (metabolism), Protein Synthesis Inhibitors (toxicity), Pyridinium Compounds (metabolism), Regeneration (drug effects), Regeneration (physiology), Time Factors, Tubulin (metabolism).
- MESH :
- chemical , metabolism : Gentamicins, Nuclear Proteins, Pyridinium Compounds, Tubulin.
- anatomy & histology : Larva.
- cytology : Lateral Line System, Mechanoreceptors.
- drug effects : Cell Proliferation, Gene Expression Regulation, Developmental, Hair Cells, Auditory, Regeneration.
- physiology : Regeneration.
- chemical , toxicity : Neomycin, Protein Synthesis Inhibitors.
- ultrastructure : Hair Cells, Auditory.
- Age Factors, Animals, Dose-Response Relationship, Drug, Embryo, Nonmammalian, Fishes, Microscopy, Electron, Scanning, Time Factors.
Abstract
The lateral line found in some amphibians and fishes has two distinctive classes of sensory organs: mechanoreceptors (neuromasts) and electroreceptors (ampullary organs). Hair cells in neuromasts can be damaged by aminoglycoside antibiotics and they will regenerate rapidly afterward. Aminoglycoside sensitivity and the capacity for regeneration have not been investigated in ampullary organs. We treated Siberian sturgeon (Acipenser baerii) larvae with neomycin and observed loss and regeneration of sensory hair cells in both organs by labeling with DASPEI and scanning electron microscopy (SEM). The numbers of sensory hair cells in both organs were reduced to the lowest levels at 6 hours posttreatment (hpt). New sensory hair cells began to appear at 12 hpt and were regenerated completely in 7 days. To reveal the possible mechanism for ampullary hair cell regeneration, we analyzed cell proliferation and the expression of neural placodal gene eya1 during regeneration. Both cell proliferation and eya1 expression were concentrated in peripheral mantle cells and both increased to the highest level at 12 hpt, which is consistent with the time course for regeneration of the ampullary hair cells. Furthermore, we used Texas Red-conjugated gentamicin in an uptake assay following pretreatment with a cation channel blocker (amiloride) and found that entry of the antibiotic was suppressed in both organs. Together, our results indicate that ampullary hair cells in Siberian sturgeon larvae can be damaged by neomycin exposure and they can regenerate rapidly. We suggest that the mechanisms for aminoglycoside uptake and hair cell regeneration are conserved for mechanoreceptors and electroreceptors. J. Comp. Neurol. 524:1443-1456, 2016. © 2015 Wiley Periodicals, Inc.
DOI: 10.1002/cne.23918
PubMed: 26502298
Affiliations:
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Le document en format XML
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Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Zou, Sha" sort="Zou, Sha" uniqKey="Zou S" first="Sha" last="Zou">Sha Zou</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Exploration and Utilization of Aquatic Genetic 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Factors</term><term>Animals</term><term>Dose-Response Relationship, Drug</term><term>Embryo, Nonmammalian</term><term>Fishes</term><term>Microscopy, Electron, Scanning</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The lateral line found in some amphibians and fishes has two distinctive classes of sensory organs: mechanoreceptors (neuromasts) and electroreceptors (ampullary organs). Hair cells in neuromasts can be damaged by aminoglycoside antibiotics and they will regenerate rapidly afterward. Aminoglycoside sensitivity and the capacity for regeneration have not been investigated in ampullary organs. We treated Siberian sturgeon (Acipenser baerii) larvae with neomycin and observed loss and regeneration of sensory hair cells in both organs by labeling with DASPEI and scanning electron microscopy (SEM). The numbers of sensory hair cells in both organs were reduced to the lowest levels at 6 hours posttreatment (hpt). New sensory hair cells began to appear at 12 hpt and were regenerated completely in 7 days. To reveal the possible mechanism for ampullary hair cell regeneration, we analyzed cell proliferation and the expression of neural placodal gene eya1 during regeneration. Both cell proliferation and eya1 expression were concentrated in peripheral mantle cells and both increased to the highest level at 12 hpt, which is consistent with the time course for regeneration of the ampullary hair cells. Furthermore, we used Texas Red-conjugated gentamicin in an uptake assay following pretreatment with a cation channel blocker (amiloride) and found that entry of the antibiotic was suppressed in both organs. Together, our results indicate that ampullary hair cells in Siberian sturgeon larvae can be damaged by neomycin exposure and they can regenerate rapidly. We suggest that the mechanisms for aminoglycoside uptake and hair cell regeneration are conserved for mechanoreceptors and electroreceptors. J. Comp. Neurol. 524:1443-1456, 2016. © 2015 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Fan, Chunxin" sort="Fan, Chunxin" uniqKey="Fan C" first="Chunxin" last="Fan">Chunxin Fan</name></noRegion><name sortKey="Song, Jiakun" sort="Song, Jiakun" uniqKey="Song J" first="Jiakun" last="Song">Jiakun Song</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name><name sortKey="Zou, Sha" sort="Zou, Sha" uniqKey="Zou S" first="Sha" last="Zou">Sha Zou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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