Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells.
Identifieur interne : 000589 ( Main/Exploration ); précédent : 000588; suivant : 000590Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells.
Auteurs : Martin Flajšhans [République tchèque] ; Martin Pšeni Ka ; Marek Rodina ; Jakub T ItelSource :
- Cell biology international [ 1095-8355 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- blood : Fishes.
- cytology : Erythrocytes.
- Animals, Cell Size, Diploidy, Image Cytometry, Tetraploidy, Triploidy.
Abstract
Comparative image cytometry of erythrocytes of diploid and triploid tench Tinca tinca L. and evolutionary tetraploid sterlet Acipenser ruthenus L. was performed on whole live unstained cells, live cells with stained nuclei and on stained fixed whole cells and their nuclei to test if erythrocyte measurements made from blood smears reflect the true dimensions of live cells. Nuclear area and perimeter were the best ploidy level predictors distinguishing accurately among live and fixed diploid, triploid and tetraploid cells, without significant differences between live and fixed cells within a ploidy level. Redundancy analysis revealed insignificant marginal effect of fixation (explained 2.3% of variation, F = 0.804), whereas the effect of ploidy level was highly significant (explained 50.6% of variation, F = 34.874). The erythrocyte measurements of diploid, triploid and tetraploid fish erythrocytes and their nuclei made from blood smears reflect the true dimensions of live cells, and the fixation procedure did not substantially affect their predictive value for ploidy level determination.
DOI: 10.1042/CBI20100198
PubMed: 20812918
Affiliations:
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sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="T Itel, Jakub" sort="T Itel, Jakub" uniqKey="T Itel J" first="Jakub" last="T Itel">Jakub T Itel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20812918</idno><idno type="pmid">20812918</idno><idno type="doi">10.1042/CBI20100198</idno><idno type="wicri:Area/PubMed/Corpus">000366</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000366</idno><idno type="wicri:Area/PubMed/Curation">000366</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000366</idno><idno type="wicri:Area/PubMed/Checkpoint">000366</idno><idno type="wicri:explorRef" 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flajshans@vurh.jcu.cz</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia esk Budjovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, 389 25 Vodany</wicri:regionArea><wicri:noRegion>389 25 Vodany</wicri:noRegion></affiliation></author><author><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="T Itel, Jakub" sort="T Itel, Jakub" uniqKey="T Itel J" first="Jakub" last="T Itel">Jakub T Itel</name></author></analytic><series><title level="j">Cell biology international</title><idno type="eISSN">1095-8355</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Size</term><term>Diploidy</term><term>Erythrocytes (cytology)</term><term>Fishes (blood)</term><term>Image Cytometry</term><term>Tetraploidy</term><term>Triploidy</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Erythrocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Size</term><term>Diploidy</term><term>Image Cytometry</term><term>Tetraploidy</term><term>Triploidy</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Comparative image cytometry of erythrocytes of diploid and triploid tench Tinca tinca L. and evolutionary tetraploid sterlet Acipenser ruthenus L. was performed on whole live unstained cells, live cells with stained nuclei and on stained fixed whole cells and their nuclei to test if erythrocyte measurements made from blood smears reflect the true dimensions of live cells. Nuclear area and perimeter were the best ploidy level predictors distinguishing accurately among live and fixed diploid, triploid and tetraploid cells, without significant differences between live and fixed cells within a ploidy level. Redundancy analysis revealed insignificant marginal effect of fixation (explained 2.3% of variation, F = 0.804), whereas the effect of ploidy level was highly significant (explained 50.6% of variation, F = 34.874). The erythrocyte measurements of diploid, triploid and tetraploid fish erythrocytes and their nuclei made from blood smears reflect the true dimensions of live cells, and the fixation procedure did not substantially affect their predictive value for ploidy level determination.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><name sortKey="T Itel, Jakub" sort="T Itel, Jakub" uniqKey="T Itel J" first="Jakub" last="T Itel">Jakub T Itel</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Flajshans, Martin" sort="Flajshans, Martin" uniqKey="Flajshans M" first="Martin" last="Flajšhans">Martin Flajšhans</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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