Osmo- and ionoregulatory responses of green sturgeon (Acipenser medirostris) to salinity acclimation.
Identifieur interne : 000893 ( Main/Exploration ); précédent : 000892; suivant : 000894Osmo- and ionoregulatory responses of green sturgeon (Acipenser medirostris) to salinity acclimation.
Auteurs : Brian A. Sardella [États-Unis] ; Dietmar KültzSource :
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology [ 1432-136X ] ; 2009.
English descriptors
- KwdEn :
- Acclimatization (physiology), Analysis of Variance, Animals, California, Caspases (metabolism), Fishes (physiology), Fresh Water, Hydrogen-Ion Concentration, Laser Scanning Cytometry, Microarray Analysis, Salinity, Seawater, Sodium-Potassium-Exchanging ATPase (metabolism), Water-Electrolyte Balance (physiology).
- MESH :
- chemical , metabolism : Caspases, Sodium-Potassium-Exchanging ATPase.
- geographic : California.
- physiology : Acclimatization, Fishes, Water-Electrolyte Balance.
- Analysis of Variance, Animals, Fresh Water, Hydrogen-Ion Concentration, Laser Scanning Cytometry, Microarray Analysis, Salinity, Seawater.
Abstract
The green sturgeon is a long-lived, highly migratory species with populations that are currently listed as threatened. Their anadromous life history requires that they make osmo- and ionoregulatory adjustments in order to maintain a consistent internal milieu as they move between fresh-, brackish-, and seawater. We acclimated juvenile green sturgeon (121 +/- 10.0 g) to 0 (freshwater; FW), 15 (estuarine; EST), and 24 g/l (SF Bay water; BAY) at 18 degrees C for 2 weeks and measured the physiological and biochemical responses with respect to osmo- and ionoregulatory mechanisms. Plasma osmolality in EST- and BAY-acclimated sturgeon was elevated relative to FW-acclimated sturgeon (P < 0.01), but there was no difference in muscle water content or abundance of stress proteins. Branchial Na(+), K(+)-ATPase (NKA) activity was also unchanged, but abundance within mitochondrion-rich cells (MRC) was greater in BAY-acclimated sturgeon (P < 0.01). FW-acclimated sturgeon had the greatest NKA abundance when assessed at the level of the entire tissue (P < 0.01), but there were no differences in v-type H(+)ATPase (VHA) activity or abundance between salinities. The Na(+), K(+), 2Cl(-) co-transporter (NKCC) was present in FW-acclimated sturgeon gills, but the overall abundance was lower relative to sturgeon in EST or BAY water (P < 0.01) where this enzyme is crucial to hypoosmoregulation. Branchial caspase 3/7 activity was significantly affected by acclimation salinity (P < 0.05) where the overall trend was for activity to increase with salinity as has been commonly observed in teleosts. Sturgeon of this age/size class were able to survive and acclimate following a salinity transfer with minimal signs of osmotic stress. The presence of the NKCC in FW-acclimated sturgeon may indicate the development of SW-readiness at this age/size.
DOI: 10.1007/s00360-008-0321-5
PubMed: 19066909
Affiliations:
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Sardella</name><affiliation wicri:level="2"><nlm:affiliation>Department of Animal Science, University of California, Davis, CA 95616, USA. basardella@ucdavis.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Animal Science, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kultz, Dietmar" sort="Kultz, Dietmar" uniqKey="Kultz D" first="Dietmar" last="Kültz">Dietmar Kültz</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19066909</idno><idno type="pmid">19066909</idno><idno type="doi">10.1007/s00360-008-0321-5</idno><idno type="wicri:Area/PubMed/Corpus">000437</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000437</idno><idno type="wicri:Area/PubMed/Curation">000437</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000437</idno><idno type="wicri:Area/PubMed/Checkpoint">000437</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000437</idno><idno type="wicri:Area/Ncbi/Merge">000272</idno><idno type="wicri:Area/Ncbi/Curation">000272</idno><idno type="wicri:Area/Ncbi/Checkpoint">000272</idno><idno type="wicri:Area/Main/Merge">000907</idno><idno type="wicri:Area/Main/Curation">000893</idno><idno type="wicri:Area/Main/Exploration">000893</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Osmo- and ionoregulatory responses of green sturgeon (Acipenser medirostris) to salinity acclimation.</title><author><name sortKey="Sardella, Brian A" sort="Sardella, Brian A" uniqKey="Sardella B" first="Brian A" last="Sardella">Brian A. Sardella</name><affiliation wicri:level="2"><nlm:affiliation>Department of Animal Science, University of California, Davis, CA 95616, USA. basardella@ucdavis.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Animal Science, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kultz, Dietmar" sort="Kultz, Dietmar" uniqKey="Kultz D" first="Dietmar" last="Kültz">Dietmar Kültz</name></author></analytic><series><title level="j">Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology</title><idno type="eISSN">1432-136X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acclimatization (physiology)</term><term>Analysis of Variance</term><term>Animals</term><term>California</term><term>Caspases (metabolism)</term><term>Fishes (physiology)</term><term>Fresh Water</term><term>Hydrogen-Ion Concentration</term><term>Laser Scanning Cytometry</term><term>Microarray Analysis</term><term>Salinity</term><term>Seawater</term><term>Sodium-Potassium-Exchanging ATPase (metabolism)</term><term>Water-Electrolyte Balance (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspases</term><term>Sodium-Potassium-Exchanging ATPase</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>California</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Acclimatization</term><term>Fishes</term><term>Water-Electrolyte Balance</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Fresh Water</term><term>Hydrogen-Ion Concentration</term><term>Laser Scanning Cytometry</term><term>Microarray Analysis</term><term>Salinity</term><term>Seawater</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The green sturgeon is a long-lived, highly migratory species with populations that are currently listed as threatened. Their anadromous life history requires that they make osmo- and ionoregulatory adjustments in order to maintain a consistent internal milieu as they move between fresh-, brackish-, and seawater. We acclimated juvenile green sturgeon (121 +/- 10.0 g) to 0 (freshwater; FW), 15 (estuarine; EST), and 24 g/l (SF Bay water; BAY) at 18 degrees C for 2 weeks and measured the physiological and biochemical responses with respect to osmo- and ionoregulatory mechanisms. Plasma osmolality in EST- and BAY-acclimated sturgeon was elevated relative to FW-acclimated sturgeon (P < 0.01), but there was no difference in muscle water content or abundance of stress proteins. Branchial Na(+), K(+)-ATPase (NKA) activity was also unchanged, but abundance within mitochondrion-rich cells (MRC) was greater in BAY-acclimated sturgeon (P < 0.01). FW-acclimated sturgeon had the greatest NKA abundance when assessed at the level of the entire tissue (P < 0.01), but there were no differences in v-type H(+)ATPase (VHA) activity or abundance between salinities. The Na(+), K(+), 2Cl(-) co-transporter (NKCC) was present in FW-acclimated sturgeon gills, but the overall abundance was lower relative to sturgeon in EST or BAY water (P < 0.01) where this enzyme is crucial to hypoosmoregulation. Branchial caspase 3/7 activity was significantly affected by acclimation salinity (P < 0.05) where the overall trend was for activity to increase with salinity as has been commonly observed in teleosts. Sturgeon of this age/size class were able to survive and acclimate following a salinity transfer with minimal signs of osmotic stress. The presence of the NKCC in FW-acclimated sturgeon may indicate the development of SW-readiness at this age/size.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Kultz, Dietmar" sort="Kultz, Dietmar" uniqKey="Kultz D" first="Dietmar" last="Kültz">Dietmar Kültz</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Sardella, Brian A" sort="Sardella, Brian A" uniqKey="Sardella B" first="Brian A" last="Sardella">Brian A. Sardella</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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