Control of flatfish sperm motility by CO2 and carbonic anhydrase
Identifieur interne : 001236 ( Main/Exploration ); précédent : 001235; suivant : 001237Control of flatfish sperm motility by CO2 and carbonic anhydrase
Auteurs : Kazuo Inaba [Japon] ; Catherine Dréanno [France] ; Jacky Cosson [France]Source :
- Cell Motility and the Cytoskeleton [ 0886-1544 ] ; 2003-07.
English descriptors
- KwdEn :
Abstract
Sperm motility in flatfishes shows unique characteristics. The flagellar movement either in vivo or in permeabilized models is arrested by the presence of 25–100 mM HCO3−, or by gentle perfusion with CO2 gas. To understand the molecular basis of this property, sperm Triton‐soluble proteins and flagellar proteins from several species were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. An abundant 29‐kDa protein was observed only in flatfish species. Partial amino acid sequences identified this protein as a carbonic anhydrase, an enzyme involved in the interconversion of CO2 and HCO3−. 6‐ethoxyzolamide, a specific inhibitor of carbonic anhydrase inhibits sperm motility, especially at low pH. In the case of HCO3−‐arrested sperm, the motility is restored by addition of 6‐ethoxyzolamide. Taken together, these results suggest that a novel pH/ HCO3−‐dependent regulatory mechanism mediated by carbonic anhydrase is involved in the motility control in flatfish sperm. Cell Motil. Cytoskeleton 55:174–187, 2003. © 2003 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/cm.10119
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C20
- to stream Istex, to step Curation: 000C18
- to stream Istex, to step Checkpoint: 000A65
- to stream Main, to step Merge: 001290
- to stream Main, to step Curation: 001236
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Control of flatfish sperm motility by CO2 and carbonic anhydrase</title>
<author><name sortKey="Inaba, Kazuo" sort="Inaba, Kazuo" uniqKey="Inaba K" first="Kazuo" last="Inaba">Kazuo Inaba</name>
</author>
<author><name sortKey="Dreanno, Catherine" sort="Dreanno, Catherine" uniqKey="Dreanno C" first="Catherine" last="Dréanno">Catherine Dréanno</name>
</author>
<author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:F7E079A96CC9E5B71BB8D28415B527BECE7BC3E6</idno>
<date when="2003" year="2003">2003</date>
<idno type="doi">10.1002/cm.10119</idno>
<idno type="url">https://api.istex.fr/document/F7E079A96CC9E5B71BB8D28415B527BECE7BC3E6/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C20</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C20</idno>
<idno type="wicri:Area/Istex/Curation">000C18</idno>
<idno type="wicri:Area/Istex/Checkpoint">000A65</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A65</idno>
<idno type="wicri:doubleKey">0886-1544:2003:Inaba K:control:of:flatfish</idno>
<idno type="wicri:Area/Main/Merge">001290</idno>
<idno type="wicri:Area/Main/Curation">001236</idno>
<idno type="wicri:Area/Main/Exploration">001236</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Control of flatfish sperm motility by CO2 and carbonic anhydrase</title>
<author><name sortKey="Inaba, Kazuo" sort="Inaba, Kazuo" uniqKey="Inaba K" first="Kazuo" last="Inaba">Kazuo Inaba</name>
<affiliation wicri:level="4"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Asamushi, Aomori</wicri:regionArea>
<orgName type="university">Université du Tōhoku</orgName>
<placeName><settlement type="city">Sendai</settlement>
<region type="province">Région de Tōhoku</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Asamushi, Aomori 039‐3501</wicri:regionArea>
<orgName type="university">Université du Tōhoku</orgName>
<placeName><settlement type="city">Sendai</settlement>
<region type="province">Région de Tōhoku</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dreanno, Catherine" sort="Dreanno, Catherine" uniqKey="Dreanno C" first="Catherine" last="Dréanno">Catherine Dréanno</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>URA 671 CNRS, University of Paris 6, Villefranche‐sur‐mer</wicri:regionArea>
<wicri:noRegion>Villefranche‐sur‐mer</wicri:noRegion>
<wicri:noRegion>Villefranche‐sur‐mer</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>URA 671 CNRS, University of Paris 6, Villefranche‐sur‐mer</wicri:regionArea>
<wicri:noRegion>Villefranche‐sur‐mer</wicri:noRegion>
<wicri:noRegion>Villefranche‐sur‐mer</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell Motility and the Cytoskeleton</title>
<title level="j" type="abbrev">Cell Motil. Cytoskeleton</title>
<idno type="ISSN">0886-1544</idno>
<idno type="eISSN">1097-0169</idno>
<imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="2003-07">2003-07</date>
<biblScope unit="volume">55</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="174">174</biblScope>
<biblScope unit="page" to="187">187</biblScope>
</imprint>
<idno type="ISSN">0886-1544</idno>
</series>
<idno type="istex">F7E079A96CC9E5B71BB8D28415B527BECE7BC3E6</idno>
<idno type="DOI">10.1002/cm.10119</idno>
<idno type="ArticleID">CM10119</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0886-1544</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>creatine kinase</term>
<term>ethoxyzolamide</term>
<term>flounder</term>
<term>sperm axoneme</term>
<term>teleost flagella</term>
<term>turbot</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Sperm motility in flatfishes shows unique characteristics. The flagellar movement either in vivo or in permeabilized models is arrested by the presence of 25–100 mM HCO3−, or by gentle perfusion with CO2 gas. To understand the molecular basis of this property, sperm Triton‐soluble proteins and flagellar proteins from several species were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. An abundant 29‐kDa protein was observed only in flatfish species. Partial amino acid sequences identified this protein as a carbonic anhydrase, an enzyme involved in the interconversion of CO2 and HCO3−. 6‐ethoxyzolamide, a specific inhibitor of carbonic anhydrase inhibits sperm motility, especially at low pH. In the case of HCO3−‐arrested sperm, the motility is restored by addition of 6‐ethoxyzolamide. Taken together, these results suggest that a novel pH/ HCO3−‐dependent regulatory mechanism mediated by carbonic anhydrase is involved in the motility control in flatfish sperm. Cell Motil. Cytoskeleton 55:174–187, 2003. © 2003 Wiley‐Liss, Inc.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Japon</li>
</country>
<region><li>Région de Tōhoku</li>
</region>
<settlement><li>Sendai</li>
</settlement>
<orgName><li>Université du Tōhoku</li>
</orgName>
</list>
<tree><country name="Japon"><region name="Région de Tōhoku"><name sortKey="Inaba, Kazuo" sort="Inaba, Kazuo" uniqKey="Inaba K" first="Kazuo" last="Inaba">Kazuo Inaba</name>
</region>
<name sortKey="Inaba, Kazuo" sort="Inaba, Kazuo" uniqKey="Inaba K" first="Kazuo" last="Inaba">Kazuo Inaba</name>
</country>
<country name="France"><noRegion><name sortKey="Dreanno, Catherine" sort="Dreanno, Catherine" uniqKey="Dreanno C" first="Catherine" last="Dréanno">Catherine Dréanno</name>
</noRegion>
<name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001236 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001236 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:F7E079A96CC9E5B71BB8D28415B527BECE7BC3E6 |texte= Control of flatfish sperm motility by CO2 and carbonic anhydrase }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |