Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus
Identifieur interne : 001930 ( Main/Exploration ); précédent : 001929; suivant : 001931Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus
Auteurs : K. M. Tham [Nouvelle-Zélande] ; C. D. Moon [Nouvelle-Zélande]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1996.
Abstract
Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.
Url:
DOI: 10.1016/0166-0934(96)02070-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000719
- to stream Istex, to step Curation: 000717
- to stream Istex, to step Checkpoint: 000F24
- to stream Main, to step Merge: 001A36
- to stream Main, to step Curation: 001930
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</title><author><name sortKey="Tham, K M" sort="Tham, K M" uniqKey="Tham K" first="K. M." last="Tham">K. M. Tham</name></author><author><name sortKey="Moon, C D" sort="Moon, C D" uniqKey="Moon C" first="C. D." last="Moon">C. D. Moon</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A77171C7A971B02A831582BFA98E2244FBF692A9</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/0166-0934(96)02070-8</idno><idno type="url">https://api.istex.fr/document/A77171C7A971B02A831582BFA98E2244FBF692A9/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000719</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000719</idno><idno type="wicri:Area/Istex/Curation">000717</idno><idno type="wicri:Area/Istex/Checkpoint">000F24</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F24</idno><idno type="wicri:doubleKey">0166-0934:1996:Tham K:polymerase:chain:reaction</idno><idno type="wicri:Area/Main/Merge">001A36</idno><idno type="wicri:Area/Main/Curation">001930</idno><idno type="wicri:Area/Main/Exploration">001930</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</title><author><name sortKey="Tham, K M" sort="Tham, K M" uniqKey="Tham K" first="K. M." last="Tham">K. M. Tham</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt</wicri:regionArea><wicri:noRegion>Upper Hutt</wicri:noRegion></affiliation></author><author><name sortKey="Moon, C D" sort="Moon, C D" uniqKey="Moon C" first="C. D." last="Moon">C. D. Moon</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt</wicri:regionArea><wicri:noRegion>Upper Hutt</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="65">65</biblScope><biblScope unit="page" to="72">72</biblScope></imprint><idno type="ISSN">0166-0934</idno></series><idno type="istex">A77171C7A971B02A831582BFA98E2244FBF692A9</idno><idno type="DOI">10.1016/0166-0934(96)02070-8</idno><idno type="PII">0166-0934(96)02070-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.</div></front></TEI><affiliations><list><country><li>Nouvelle-Zélande</li></country></list><tree><country name="Nouvelle-Zélande"><noRegion><name sortKey="Tham, K M" sort="Tham, K M" uniqKey="Tham K" first="K. M." last="Tham">K. M. Tham</name></noRegion><name sortKey="Moon, C D" sort="Moon, C D" uniqKey="Moon C" first="C. D." last="Moon">C. D. Moon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001930 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001930 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:A77171C7A971B02A831582BFA98E2244FBF692A9
|texte= Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus
}}
| This area was generated with Dilib version V0.6.27. |  |