Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17β‐estradiol treatment
Identifieur interne : 001561 ( Main/Exploration ); précédent : 001560; suivant : 001562Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17β‐estradiol treatment
Auteurs : Joseph J. Korte [États-Unis] ; Michael D. Kahl [États-Unis] ; Kathleen M. Jensen [États-Unis] ; Mumtaz S. Pasha [États-Unis] ; Louise G. Parks [États-Unis] ; Gerald A. Leblanc [États-Unis] ; Gerald T. Ankley [États-Unis]Source :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2000-04.
English descriptors
- KwdEn :
Abstract
Induction of vitellogenin (VTG) in oviparous animals has been proposed as a sensitive indicator of environmental contaminants that activate the estrogen receptor. In the present study, a sensitive ribonuclease protection assay (RPA) for VTG messenger RNA (mRNA) was developed for the fathead minnow (Pimephales promelas), a species proposed for routine endocrine‐disrupting chemical (EDC) screening. The utility of this method was compared with an enzyme‐linked immunosorbent assay (ELISA) specific for fathead minnow VTG protein. Assessment of the two methods included kinetic characterization of the plasma VTG protein and hepatic VTG mRNA levels in male fathead minnows following intraperitoneal injections of 17β‐estradiol (E2) at two dose levels (0.5, 5.0 mg/kg). Initial plasma E2 concentrations were elevated in a dose‐dependent manner but returned to normal levels within 2 d. Liver VTG mRNA was detected within 4 h, reached a maximum around 48 h, and returned to normal levels in about 6 d. Plasma VTG protein was detectable within 16 h of treatment, reached maximum levels at about 72 h, and remained near these maximum levels for at least 18 d. While the RPA was about 1,000 times more sensitive than the ELISA, the ELISA appears superior for routine screening tests. The ELISA method is relatively simple to perform and, because males lack a clearance mechanism for VTG, the protein remains at relatively high concentrations in the plasma for an extended period of time. As part of the development of the RPA, the complementary DNA (cDNA) sequence for fathead minnow VTG was determined and the deduced amino acid sequence compared with VTG sequences for other fish species.
Url:
DOI: 10.1002/etc.5620190426
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001283
- to stream Istex, to step Curation: 001281
- to stream Istex, to step Checkpoint: 000C87
- to stream Main, to step Merge: 001638
- to stream Main, to step Curation: 001561
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17β‐estradiol treatment</title><author><name sortKey="Korte, Joseph J" sort="Korte, Joseph J" uniqKey="Korte J" first="Joseph J." last="Korte">Joseph J. Korte</name></author><author><name sortKey="Kahl, Michael D" sort="Kahl, Michael D" uniqKey="Kahl M" first="Michael D." last="Kahl">Michael D. Kahl</name></author><author><name sortKey="Jensen, Kathleen M" sort="Jensen, Kathleen M" uniqKey="Jensen K" first="Kathleen M." last="Jensen">Kathleen M. Jensen</name></author><author><name sortKey="Pasha, Mumtaz S" sort="Pasha, Mumtaz S" uniqKey="Pasha M" first="Mumtaz S." last="Pasha">Mumtaz S. Pasha</name></author><author><name sortKey="Parks, Louise G" sort="Parks, Louise G" uniqKey="Parks L" first="Louise G." last="Parks">Louise G. Parks</name></author><author><name sortKey="Leblanc, Gerald A" sort="Leblanc, Gerald A" uniqKey="Leblanc G" first="Gerald A." last="Leblanc">Gerald A. Leblanc</name></author><author><name sortKey="Ankley, Gerald T" sort="Ankley, Gerald T" uniqKey="Ankley G" first="Gerald T." last="Ankley">Gerald T. Ankley</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9946833BC01C6C6246CB9E2EB5F6EE3D42829124</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1002/etc.5620190426</idno><idno type="url">https://api.istex.fr/document/9946833BC01C6C6246CB9E2EB5F6EE3D42829124/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001283</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001283</idno><idno type="wicri:Area/Istex/Curation">001281</idno><idno type="wicri:Area/Istex/Checkpoint">000C87</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C87</idno><idno type="wicri:doubleKey">0730-7268:2000:Korte J:fathead:minnow:vitellogenin</idno><idno type="wicri:Area/Main/Merge">001638</idno><idno type="wicri:Area/Main/Curation">001561</idno><idno type="wicri:Area/Main/Exploration">001561</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17β‐estradiol treatment</title><author><name sortKey="Korte, Joseph J" sort="Korte, Joseph J" uniqKey="Korte J" first="Joseph J." last="Korte">Joseph J. Korte</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>U.S. Environmental Protection Agency, Mid‐Continent Ecology Division, 6201 Congdon Boulevard, Duluth</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>U.S. Environmental Protection Agency, Mid‐Continent Ecology Division, 6201 Congdon Boulevard, Duluth</wicri:cityArea></affiliation></author><author><name sortKey="Kahl, Michael D" sort="Kahl, Michael D" uniqKey="Kahl M" first="Michael D." last="Kahl">Michael D. Kahl</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>U.S. Environmental Protection Agency, Mid‐Continent Ecology Division, 6201 Congdon Boulevard, Duluth</wicri:cityArea></affiliation></author><author><name sortKey="Jensen, Kathleen M" sort="Jensen, Kathleen M" uniqKey="Jensen K" first="Kathleen M." last="Jensen">Kathleen M. Jensen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>U.S. Environmental Protection Agency, Mid‐Continent Ecology Division, 6201 Congdon Boulevard, Duluth</wicri:cityArea></affiliation></author><author><name sortKey="Pasha, Mumtaz S" sort="Pasha, Mumtaz S" uniqKey="Pasha M" first="Mumtaz S." last="Pasha">Mumtaz S. Pasha</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>U.S. Environmental Protection Agency, Mid‐Continent Ecology Division, 6201 Congdon Boulevard, Duluth</wicri:cityArea></affiliation></author><author><name sortKey="Parks, Louise G" sort="Parks, Louise G" uniqKey="Parks L" first="Louise G." last="Parks">Louise G. Parks</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>North Carolina State University Department of Toxicology, Box 7633, Raleigh, North Carolina 27695</wicri:regionArea><wicri:noRegion>North Carolina 27695</wicri:noRegion></affiliation></author><author><name sortKey="Leblanc, Gerald A" sort="Leblanc, Gerald A" uniqKey="Leblanc G" first="Gerald A." last="Leblanc">Gerald A. Leblanc</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>North Carolina State University Department of Toxicology, Box 7633, Raleigh, North Carolina 27695</wicri:regionArea><wicri:noRegion>North Carolina 27695</wicri:noRegion></affiliation></author><author><name sortKey="Ankley, Gerald T" sort="Ankley, Gerald T" uniqKey="Ankley G" first="Gerald T." last="Ankley">Gerald T. Ankley</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>U.S. Environmental Protection Agency, Mid‐Continent Ecology Division, 6201 Congdon Boulevard, Duluth</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental Toxicology and Chemistry</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Environmental Toxicology and Chemistry</title><idno type="ISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><imprint><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2000-04">2000-04</date><biblScope unit="volume">19</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="972">972</biblScope><biblScope unit="page" to="981">981</biblScope></imprint><idno type="ISSN">0730-7268</idno></series><idno type="istex">9946833BC01C6C6246CB9E2EB5F6EE3D42829124</idno><idno type="DOI">10.1002/etc.5620190426</idno><idno type="ArticleID">ETC5620190426</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0730-7268</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>17β‐Estradiol</term><term>Pimephales promelas</term><term>Vitellogenin</term><term>cDNA</term><term>mRNA</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Induction of vitellogenin (VTG) in oviparous animals has been proposed as a sensitive indicator of environmental contaminants that activate the estrogen receptor. In the present study, a sensitive ribonuclease protection assay (RPA) for VTG messenger RNA (mRNA) was developed for the fathead minnow (Pimephales promelas), a species proposed for routine endocrine‐disrupting chemical (EDC) screening. The utility of this method was compared with an enzyme‐linked immunosorbent assay (ELISA) specific for fathead minnow VTG protein. Assessment of the two methods included kinetic characterization of the plasma VTG protein and hepatic VTG mRNA levels in male fathead minnows following intraperitoneal injections of 17β‐estradiol (E2) at two dose levels (0.5, 5.0 mg/kg). Initial plasma E2 concentrations were elevated in a dose‐dependent manner but returned to normal levels within 2 d. Liver VTG mRNA was detected within 4 h, reached a maximum around 48 h, and returned to normal levels in about 6 d. Plasma VTG protein was detectable within 16 h of treatment, reached maximum levels at about 72 h, and remained near these maximum levels for at least 18 d. While the RPA was about 1,000 times more sensitive than the ELISA, the ELISA appears superior for routine screening tests. The ELISA method is relatively simple to perform and, because males lack a clearance mechanism for VTG, the protein remains at relatively high concentrations in the plasma for an extended period of time. As part of the development of the RPA, the complementary DNA (cDNA) sequence for fathead minnow VTG was determined and the deduced amino acid sequence compared with VTG sequences for other fish species.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Minnesota</li></region></list><tree><country name="États-Unis"><region name="Minnesota"><name sortKey="Korte, Joseph J" sort="Korte, Joseph J" uniqKey="Korte J" first="Joseph J." last="Korte">Joseph J. Korte</name></region><name sortKey="Ankley, Gerald T" sort="Ankley, Gerald T" uniqKey="Ankley G" first="Gerald T." last="Ankley">Gerald T. Ankley</name><name sortKey="Jensen, Kathleen M" sort="Jensen, Kathleen M" uniqKey="Jensen K" first="Kathleen M." last="Jensen">Kathleen M. Jensen</name><name sortKey="Kahl, Michael D" sort="Kahl, Michael D" uniqKey="Kahl M" first="Michael D." last="Kahl">Michael D. Kahl</name><name sortKey="Korte, Joseph J" sort="Korte, Joseph J" uniqKey="Korte J" first="Joseph J." last="Korte">Joseph J. Korte</name><name sortKey="Leblanc, Gerald A" sort="Leblanc, Gerald A" uniqKey="Leblanc G" first="Gerald A." last="Leblanc">Gerald A. Leblanc</name><name sortKey="Parks, Louise G" sort="Parks, Louise G" uniqKey="Parks L" first="Louise G." last="Parks">Louise G. Parks</name><name sortKey="Pasha, Mumtaz S" sort="Pasha, Mumtaz S" uniqKey="Pasha M" first="Mumtaz S." last="Pasha">Mumtaz S. Pasha</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001561 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001561 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:9946833BC01C6C6246CB9E2EB5F6EE3D42829124
|texte= Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17β‐estradiol treatment
}}
| This area was generated with Dilib version V0.6.27. |  |