Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri.
Identifieur interne : 002574 ( Main/Exploration ); précédent : 002573; suivant : 002575Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri.
Auteurs : M L Lundqvist [Suède] ; L. PilströmSource :
- Developmental and comparative immunology [ 0145-305X ]
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Immunoglobulin Light Chains, Immunoglobulin Variable Region.
- genetics : Fishes.
- immunology : Fishes.
- Animals, Mice.
Abstract
All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.
PubMed: 10579389
Affiliations:
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Le document en format XML
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Pilström</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>1999 Oct-Dec</MedlineDate></PubDate></date><idno type="RBID">pubmed:10579389</idno><idno type="pmid">10579389</idno><idno type="wicri:Area/PubMed/Corpus">000654</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000654</idno><idno type="wicri:Area/PubMed/Curation">000654</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000654</idno><idno type="wicri:Area/PubMed/Checkpoint">000654</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000654</idno><idno type="wicri:Area/Ncbi/Merge">000010</idno><idno type="wicri:Area/Ncbi/Curation">000010</idno><idno type="wicri:Area/Ncbi/Checkpoint">000010</idno><idno type="wicri:doubleKey">0145-305X::Lundqvist M:variability:of:the</idno><idno type="wicri:Area/Main/Merge">002776</idno><idno type="wicri:Area/Main/Curation">002574</idno><idno type="wicri:Area/Main/Exploration">002574</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri.</title><author><name sortKey="Lundqvist, M L" sort="Lundqvist, M L" uniqKey="Lundqvist M" first="M L" last="Lundqvist">M L Lundqvist</name><affiliation wicri:level="4"><nlm:affiliation>Department of Cell and Molecular Biology, Uppsala University, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Cell and Molecular Biology, Uppsala University</wicri:regionArea><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName><orgName type="university">Université d'Uppsala</orgName></affiliation></author><author><name sortKey="Pilstrom, L" sort="Pilstrom, L" uniqKey="Pilstrom L" first="L" last="Pilström">L. Pilström</name></author></analytic><series><title level="j">Developmental and comparative immunology</title><idno type="ISSN">0145-305X</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Fishes (genetics)</term><term>Fishes (immunology)</term><term>Immunoglobulin Light Chains (genetics)</term><term>Immunoglobulin Variable Region (genetics)</term><term>Mice</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Immunoglobulin Light Chains</term><term>Immunoglobulin Variable Region</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Mice</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>East Middle Sweden</li><li>Svealand</li></region><settlement><li>Uppsala</li></settlement><orgName><li>Université d'Uppsala</li></orgName></list><tree><noCountry><name sortKey="Pilstrom, L" sort="Pilstrom, L" uniqKey="Pilstrom L" first="L" last="Pilström">L. Pilström</name></noCountry><country name="Suède"><region name="Svealand"><name sortKey="Lundqvist, M L" sort="Lundqvist, M L" uniqKey="Lundqvist M" first="M L" last="Lundqvist">M L Lundqvist</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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