Mitochondrial DNA length variation and heteroplasmy in populations of white sturgeon (Acipenser transmontanus)
Identifieur interne : 001C62 ( Main/Exploration ); précédent : 001C61; suivant : 001C63Mitochondrial DNA length variation and heteroplasmy in populations of white sturgeon (Acipenser transmontanus)
Auteurs : J. R. Brown [Canada] ; A. T. Beckenbach ; M. J. SmithSource :
- Genetics [ 0016-6731 ] ; 1992.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Milieu marin.
English descriptors
- KwdEn :
- Acipenser transmontanus, Animals, Base Sequence, Blotting, Southern, Copy number, DNA Probes, DNA, Mitochondrial (genetics), Fishes (genetics), Gene frequency, Genotype, Marine environment, Mitochondrial DNA, Molecular Sequence Data, Phylogeny, Polymorphism, Genetic (genetics), Restriction fragment length polymorphism, Tandemly repeated sequence.
- MESH :
- chemical , genetics : DNA, Mitochondrial.
- chemical : DNA Probes.
- genetics : Fishes, Polymorphism, Genetic.
- Animals, Base Sequence, Blotting, Southern, Genotype, Molecular Sequence Data, Phylogeny.
Abstract
Southern blot analysis was used to quantify the extent of mtDNA D-loop length variation in two populations of white sturgeon, Acipenser transmontanus. Over 42% of individuals were heteroplasmic for up to six different mtDNA length variants attributable to varying copy numbers of an 82-bp repeat sequence. Chi-square analyses revealed that the frequencies of length genotypes and the incidence of heteroplasmy were significantly different between Fraser and Columbia River sturgeon populations but not between restriction site haplotypes. Heteroplasmic fish have, on average, higher copy number than homoplasmic fish
Affiliations:
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R." last="Brown">J. R. Brown</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Simon Fraser univ., inst. molecular biology biochemistry, dep. biological sci.</s1><s2>Burnaby BC V5A 1S6</s2><s3>CAN</s3></inist:fA14><country>Canada</country><wicri:noRegion>Burnaby BC V5A 1S6</wicri:noRegion></affiliation></author><author><name sortKey="Beckenbach, A T" sort="Beckenbach, A T" uniqKey="Beckenbach A" first="A. T." last="Beckenbach">A. T. Beckenbach</name></author><author><name sortKey="Smith, M J" sort="Smith, M J" uniqKey="Smith M" first="M. J." last="Smith">M. J. Smith</name></author></analytic><series><title level="j" type="main">Genetics</title><title level="j" type="abbreviated">Genetics</title><idno type="ISSN">0016-6731</idno><imprint><date when="1992">1992</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Genetics</title><title level="j" type="abbreviated">Genetics</title><idno type="ISSN">0016-6731</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term><term>Animals</term><term>Base Sequence</term><term>Blotting, Southern</term><term>Copy number</term><term>DNA Probes</term><term>DNA, Mitochondrial (genetics)</term><term>Fishes (genetics)</term><term>Gene frequency</term><term>Genotype</term><term>Marine environment</term><term>Mitochondrial DNA</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Polymorphism, Genetic (genetics)</term><term>Restriction fragment length polymorphism</term><term>Tandemly repeated sequence</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Mitochondrial</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Probes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term><term>Polymorphism, Genetic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Blotting, Southern</term><term>Genotype</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Acipenser transmontanus</term><term>Milieu marin</term><term>Séquence répétée en tandem</term><term>DNA mitochondrial</term><term>Nombre copie</term><term>Polymorphisme longueur fragment restriction</term><term>Fréquence génique</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Milieu marin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Southern blot analysis was used to quantify the extent of mtDNA D-loop length variation in two populations of white sturgeon, Acipenser transmontanus. Over 42% of individuals were heteroplasmic for up to six different mtDNA length variants attributable to varying copy numbers of an 82-bp repeat sequence. Chi-square analyses revealed that the frequencies of length genotypes and the incidence of heteroplasmy were significantly different between Fraser and Columbia River sturgeon populations but not between restriction site haplotypes. Heteroplasmic fish have, on average, higher copy number than homoplasmic fish</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Beckenbach, A T" sort="Beckenbach, A T" uniqKey="Beckenbach A" first="A. T." last="Beckenbach">A. T. Beckenbach</name><name sortKey="Smith, M J" sort="Smith, M J" uniqKey="Smith M" first="M. J." last="Smith">M. J. Smith</name></noCountry><country name="Canada"><noRegion><name sortKey="Brown, J R" sort="Brown, J R" uniqKey="Brown J" first="J. R." last="Brown">J. R. Brown</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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