Steroidogenic acute regulatory protein in white sturgeon (Acipenser transmontanus): cDNA cloning, sites of expression and transcript abundance in corticosteroidogenic tissue after an acute stressor.
Identifieur interne : 000888 ( Main/Exploration ); précédent : 000887; suivant : 000889Steroidogenic acute regulatory protein in white sturgeon (Acipenser transmontanus): cDNA cloning, sites of expression and transcript abundance in corticosteroidogenic tissue after an acute stressor.
Auteurs : Makoto Kusakabe [États-Unis] ; Micah D. Zuccarelli ; Ikumi Nakamura ; Graham YoungSource :
- General and comparative endocrinology [ 1095-6840 ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cloning, Molecular, DNA, Complementary (chemistry), Fish Proteins (chemistry), Fish Proteins (genetics), Fish Proteins (metabolism), Fishes (genetics), Fishes (metabolism), In Situ Hybridization, Molecular Sequence Data, Phosphoproteins (chemistry), Phosphoproteins (genetics), Phosphoproteins (metabolism), Phylogeny, RNA, Messenger (metabolism), Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Stress, Physiological.
- MESH :
- chemical , chemistry : DNA, Complementary, Fish Proteins, Phosphoproteins.
- chemical , genetics : Fish Proteins, Phosphoproteins.
- chemical , metabolism : Fish Proteins, Phosphoproteins, RNA, Messenger.
- genetics : Fishes.
- metabolism : Fishes.
- Amino Acid Sequence, Animals, Cloning, Molecular, In Situ Hybridization, Molecular Sequence Data, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Stress, Physiological.
Abstract
The white sturgeon, Acipenser transmontanus, is a primitive bony fish that is recognized as an important emerging species for aquaculture. However, many aspects of its stress and reproductive physiology remain unclear. These processes are controlled by various steroid hormones. In order to investigate the regulation of steroidogenesis associated with acute stress in sturgeon, a cDNA-encoding steroidogenic acute regulatory protein (StAR) was isolated from white sturgeon. The putative amino acid sequence of sturgeon StAR shares high homology (over 60%) with other vertebrates. Phylogenetic analysis grouped sturgeon StAR within Actinopterygii, but it was clearly segregated from teleost StARs. RT-PCR analysis revealed that transcripts were most abundant in yellow corpuscles found throughout the kidney and weaker signals were detected in gonad and kidney. Very weak signals were also detected in brain and spleen by quantitative real-time PCR. In situ hybridization revealed that StAR is expressed in the cells of yellow corpuscles. No significant changes in StAR gene expression were detected in response to an acute handling stress. These results suggest that StAR is highly conserved throughout vertebrates, but the expression of the functional protein during the stress response may be partially regulated post-transcriptionally.
DOI: 10.1016/j.ygcen.2009.02.007
PubMed: 19245813
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000430
- to stream PubMed, to step Curation: 000430
- to stream PubMed, to step Checkpoint: 000430
- to stream Ncbi, to step Merge: 000277
- to stream Ncbi, to step Curation: 000277
- to stream Ncbi, to step Checkpoint: 000277
- to stream Main, to step Merge: 000902
- to stream Main, to step Curation: 000888
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Steroidogenic acute regulatory protein in white sturgeon (Acipenser transmontanus): cDNA cloning, sites of expression and transcript abundance in corticosteroidogenic tissue after an acute stressor.</title><author><name sortKey="Kusakabe, Makoto" sort="Kusakabe, Makoto" uniqKey="Kusakabe M" first="Makoto" last="Kusakabe">Makoto Kusakabe</name><affiliation wicri:level="4"><nlm:affiliation>School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USA. kusakabe@ori.u-tokyo.ac.jp</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Zuccarelli, Micah D" sort="Zuccarelli, Micah D" uniqKey="Zuccarelli M" first="Micah D" last="Zuccarelli">Micah D. Zuccarelli</name></author><author><name sortKey="Nakamura, Ikumi" sort="Nakamura, Ikumi" uniqKey="Nakamura I" first="Ikumi" last="Nakamura">Ikumi Nakamura</name></author><author><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19245813</idno><idno type="pmid">19245813</idno><idno type="doi">10.1016/j.ygcen.2009.02.007</idno><idno type="wicri:Area/PubMed/Corpus">000430</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000430</idno><idno type="wicri:Area/PubMed/Curation">000430</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000430</idno><idno type="wicri:Area/PubMed/Checkpoint">000430</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000430</idno><idno type="wicri:Area/Ncbi/Merge">000277</idno><idno type="wicri:Area/Ncbi/Curation">000277</idno><idno type="wicri:Area/Ncbi/Checkpoint">000277</idno><idno type="wicri:Area/Main/Merge">000902</idno><idno type="wicri:Area/Main/Curation">000888</idno><idno type="wicri:Area/Main/Exploration">000888</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Steroidogenic acute regulatory protein in white sturgeon (Acipenser transmontanus): cDNA cloning, sites of expression and transcript abundance in corticosteroidogenic tissue after an acute stressor.</title><author><name sortKey="Kusakabe, Makoto" sort="Kusakabe, Makoto" uniqKey="Kusakabe M" first="Makoto" last="Kusakabe">Makoto Kusakabe</name><affiliation wicri:level="4"><nlm:affiliation>School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USA. kusakabe@ori.u-tokyo.ac.jp</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Zuccarelli, Micah D" sort="Zuccarelli, Micah D" uniqKey="Zuccarelli M" first="Micah D" last="Zuccarelli">Micah D. Zuccarelli</name></author><author><name sortKey="Nakamura, Ikumi" sort="Nakamura, Ikumi" uniqKey="Nakamura I" first="Ikumi" last="Nakamura">Ikumi Nakamura</name></author><author><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name></author></analytic><series><title level="j">General and comparative endocrinology</title><idno type="eISSN">1095-6840</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>DNA, Complementary (chemistry)</term><term>Fish Proteins (chemistry)</term><term>Fish Proteins (genetics)</term><term>Fish Proteins (metabolism)</term><term>Fishes (genetics)</term><term>Fishes (metabolism)</term><term>In Situ Hybridization</term><term>Molecular Sequence Data</term><term>Phosphoproteins (chemistry)</term><term>Phosphoproteins (genetics)</term><term>Phosphoproteins (metabolism)</term><term>Phylogeny</term><term>RNA, Messenger (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term><term>Fish Proteins</term><term>Phosphoproteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fish Proteins</term><term>Phosphoproteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fish Proteins</term><term>Phosphoproteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>In Situ Hybridization</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Stress, Physiological</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The white sturgeon, Acipenser transmontanus, is a primitive bony fish that is recognized as an important emerging species for aquaculture. However, many aspects of its stress and reproductive physiology remain unclear. These processes are controlled by various steroid hormones. In order to investigate the regulation of steroidogenesis associated with acute stress in sturgeon, a cDNA-encoding steroidogenic acute regulatory protein (StAR) was isolated from white sturgeon. The putative amino acid sequence of sturgeon StAR shares high homology (over 60%) with other vertebrates. Phylogenetic analysis grouped sturgeon StAR within Actinopterygii, but it was clearly segregated from teleost StARs. RT-PCR analysis revealed that transcripts were most abundant in yellow corpuscles found throughout the kidney and weaker signals were detected in gonad and kidney. Very weak signals were also detected in brain and spleen by quantitative real-time PCR. In situ hybridization revealed that StAR is expressed in the cells of yellow corpuscles. No significant changes in StAR gene expression were detected in response to an acute handling stress. These results suggest that StAR is highly conserved throughout vertebrates, but the expression of the functional protein during the stress response may be partially regulated post-transcriptionally.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><noCountry><name sortKey="Nakamura, Ikumi" sort="Nakamura, Ikumi" uniqKey="Nakamura I" first="Ikumi" last="Nakamura">Ikumi Nakamura</name><name sortKey="Young, Graham" sort="Young, Graham" uniqKey="Young G" first="Graham" last="Young">Graham Young</name><name sortKey="Zuccarelli, Micah D" sort="Zuccarelli, Micah D" uniqKey="Zuccarelli M" first="Micah D" last="Zuccarelli">Micah D. Zuccarelli</name></noCountry><country name="États-Unis"><region name="Washington (État)"><name sortKey="Kusakabe, Makoto" sort="Kusakabe, Makoto" uniqKey="Kusakabe M" first="Makoto" last="Kusakabe">Makoto Kusakabe</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000888 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000888 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19245813
|texte= Steroidogenic acute regulatory protein in white sturgeon (Acipenser transmontanus): cDNA cloning, sites of expression and transcript abundance in corticosteroidogenic tissue after an acute stressor.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19245813" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |