[Effects of storage medium and temperature on short-term storage of Acipenser baerii eggs].
Identifieur interne : 000750 ( Main/Exploration ); précédent : 000749; suivant : 000751[Effects of storage medium and temperature on short-term storage of Acipenser baerii eggs].
Auteurs : Tao Zhang [République populaire de Chine] ; Shi-Wei Yan ; Ping Zhuang ; Long-Zhen Zhang ; Mei-Ping Tian ; Bin Wang ; Da-Hai Zhang ; Zhi-Feng YaoSource :
- Ying yong sheng tai xue bao = The journal of applied ecology [ 1001-9332 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- chemical : Culture Media.
- geographic : Siberia.
- methods : Tissue Preservation.
- physiology : Fishes, Ovum.
- veterinary : Tissue Preservation.
- Animals, Temperature.
Abstract
This paper studied the effects of different storage medium (coelomic fluid, Hepes solution, and modified Ringer's solution), temperature (4 degrees C and 16 degrees C), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of Siberian sturgeon (Acipenser baerii) eggs. The results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (P < 0.05). With the increase of storage duration, the fertilization and hatching rates decreased, while the malformation rate of hatched larvae increased. The A. baerii eggs stored at 16 degrees C had higher fertilization, hatching, and malformation rates but shorter holding time than those stored at 4 degrees C. The optimal conditions for the short-term storage of A. baerii eggs were Hepes solution prepared according to the biochemical composition of A. baerii coelomic fluid, 16 degrees C, and 4 h, under which, 86.36% fertilization rate, 94.74% hatching rate, and zero malformation would be obtained.
PubMed: 20387448
Affiliations:
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Le document en format XML
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<author><name sortKey="Zhang, Long Zhen" sort="Zhang, Long Zhen" uniqKey="Zhang L" first="Long-Zhen" last="Zhang">Long-Zhen Zhang</name>
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<author><name sortKey="Zhang, Da Hai" sort="Zhang, Da Hai" uniqKey="Zhang D" first="Da-Hai" last="Zhang">Da-Hai Zhang</name>
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<series><title level="j">Ying yong sheng tai xue bao = The journal of applied ecology</title>
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<term>Temperature</term>
<term>Tissue Preservation (methods)</term>
<term>Tissue Preservation (veterinary)</term>
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<front><div type="abstract" xml:lang="en">This paper studied the effects of different storage medium (coelomic fluid, Hepes solution, and modified Ringer's solution), temperature (4 degrees C and 16 degrees C), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of Siberian sturgeon (Acipenser baerii) eggs. The results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (P < 0.05). With the increase of storage duration, the fertilization and hatching rates decreased, while the malformation rate of hatched larvae increased. The A. baerii eggs stored at 16 degrees C had higher fertilization, hatching, and malformation rates but shorter holding time than those stored at 4 degrees C. The optimal conditions for the short-term storage of A. baerii eggs were Hepes solution prepared according to the biochemical composition of A. baerii coelomic fluid, 16 degrees C, and 4 h, under which, 86.36% fertilization rate, 94.74% hatching rate, and zero malformation would be obtained.</div>
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<name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name>
<name sortKey="Yan, Shi Wei" sort="Yan, Shi Wei" uniqKey="Yan S" first="Shi-Wei" last="Yan">Shi-Wei Yan</name>
<name sortKey="Yao, Zhi Feng" sort="Yao, Zhi Feng" uniqKey="Yao Z" first="Zhi-Feng" last="Yao">Zhi-Feng Yao</name>
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<name sortKey="Zhang, Long Zhen" sort="Zhang, Long Zhen" uniqKey="Zhang L" first="Long-Zhen" last="Zhang">Long-Zhen Zhang</name>
<name sortKey="Zhuang, Ping" sort="Zhuang, Ping" uniqKey="Zhuang P" first="Ping" last="Zhuang">Ping Zhuang</name>
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