Capacity for intracellular pH compensation during hypercapnia in white sturgeon primary liver cells.
Identifieur interne : 000597 ( Main/Exploration ); précédent : 000596; suivant : 000598Capacity for intracellular pH compensation during hypercapnia in white sturgeon primary liver cells.
Auteurs : Khuong Tuyen Huynh [Canada] ; Daniel W. Baker ; Robert Harris ; John Church ; Colin J. BraunerSource :
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology [ 1432-136X ] ; 2011.
English descriptors
- KwdEn :
- Acidosis (blood), Acidosis (metabolism), Animals, Carbon Dioxide (blood), Carbon Dioxide (metabolism), Cells, Cultured, Fishes (physiology), Hepatocytes (cytology), Hepatocytes (metabolism), Hydrogen-Ion Concentration, Hypercapnia (blood), Hypercapnia (metabolism), Hypercapnia (pathology), Intracellular Membranes (metabolism).
- MESH :
- chemical , blood : Carbon Dioxide.
- blood : Acidosis, Hypercapnia.
- cytology : Hepatocytes.
- metabolism : Acidosis, Carbon Dioxide, Hepatocytes, Hypercapnia, Intracellular Membranes.
- pathology : Hypercapnia.
- physiology : Fishes.
- Animals, Cells, Cultured, Hydrogen-Ion Concentration.
Abstract
Fish, exposed to elevated water CO(2), experience a rapid elevation in blood CO(2) (hypercapnia), resulting in acidification of both intra- and extra-cellular compartments. White sturgeon, Acipenser transmontanus, are exceptionally CO(2) tolerant and can regulate tissue intracellular pH (pH(i)) in the presence of a pronounced hypercapnic acidosis (preferential pH(i) regulation). In this study, pH(i) regulatory capacity of sturgeon liver cells in primary culture was examined to assess the suitability of employing this in vitro system to understand in vivo CO(2) tolerance in sturgeon. Using the pH-sensitive fluoroprobe BCECF, real-time changes in resting pH(i) and rates of pH(i) recovery were investigated during exposure to hypercapnia (3 and 6% CO(2)) in the absence and presence of additional acid loads induced by (20 mM) ammonium prepulse. During short-term (10 min) exposure to hypercapnia (3 and 6% CO(2)), sturgeon cells were acidified and no pH(i) compensation was observed. However, when exposure to 6% CO(2) was extended to over 19 h, the CO(2)-induced intracellular acidosis was partially compensated by a pH(i) increase of over 0.2 pH unit despite the sustained extracellular acidosis, indicative of a capacity for preferential pH(i) regulation in vitro. Since this capacity in sturgeon liver is present both in vivo and in vitro, the transmembrane transporters involved may be the same. Therefore, cell culture may be a suitable tool to identify the transporters (i.e., the cellular mechanisms underlying in vivo CO(2) tolerance) in white sturgeon and possibly in other hypercapnia-tolerant species.
DOI: 10.1007/s00360-011-0579-x
PubMed: 21519877
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Capacity for intracellular pH compensation during hypercapnia in white sturgeon primary liver cells.</title><author><name sortKey="Huynh, Khuong Tuyen" sort="Huynh, Khuong Tuyen" uniqKey="Huynh K" first="Khuong Tuyen" last="Huynh">Khuong Tuyen Huynh</name><affiliation wicri:level="1"><nlm:affiliation>Department of Zoology, University of British Columbia, 6270, University Boulevard, Vancouver, BC, V6T 1Z4, Canada. huynh@zoology.ubc.ca</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Zoology, University of British Columbia, 6270, University Boulevard, Vancouver, BC, V6T 1Z4</wicri:regionArea><wicri:noRegion>V6T 1Z4</wicri:noRegion></affiliation></author><author><name sortKey="Baker, Daniel W" sort="Baker, Daniel W" uniqKey="Baker D" first="Daniel W" last="Baker">Daniel W. Baker</name></author><author><name sortKey="Harris, Robert" sort="Harris, Robert" uniqKey="Harris R" first="Robert" last="Harris">Robert Harris</name></author><author><name sortKey="Church, John" sort="Church, John" uniqKey="Church J" first="John" last="Church">John Church</name></author><author><name sortKey="Brauner, Colin J" sort="Brauner, Colin J" uniqKey="Brauner C" first="Colin J" last="Brauner">Colin J. Brauner</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21519877</idno><idno type="pmid">21519877</idno><idno type="doi">10.1007/s00360-011-0579-x</idno><idno type="wicri:Area/PubMed/Corpus">000343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000343</idno><idno type="wicri:Area/PubMed/Curation">000343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000343</idno><idno type="wicri:Area/PubMed/Checkpoint">000343</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000343</idno><idno type="wicri:Area/Ncbi/Merge">000377</idno><idno type="wicri:Area/Ncbi/Curation">000377</idno><idno type="wicri:Area/Ncbi/Checkpoint">000377</idno><idno type="wicri:Area/Main/Merge">000607</idno><idno type="wicri:Area/Main/Curation">000597</idno><idno type="wicri:Area/Main/Exploration">000597</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Capacity for intracellular pH compensation during hypercapnia in white sturgeon primary liver cells.</title><author><name sortKey="Huynh, Khuong Tuyen" sort="Huynh, Khuong Tuyen" uniqKey="Huynh K" first="Khuong Tuyen" last="Huynh">Khuong Tuyen Huynh</name><affiliation wicri:level="1"><nlm:affiliation>Department of Zoology, University of British Columbia, 6270, University Boulevard, Vancouver, BC, V6T 1Z4, Canada. huynh@zoology.ubc.ca</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Zoology, University of British Columbia, 6270, University Boulevard, Vancouver, BC, V6T 1Z4</wicri:regionArea><wicri:noRegion>V6T 1Z4</wicri:noRegion></affiliation></author><author><name sortKey="Baker, Daniel W" sort="Baker, Daniel W" uniqKey="Baker D" first="Daniel W" last="Baker">Daniel W. Baker</name></author><author><name sortKey="Harris, Robert" sort="Harris, Robert" uniqKey="Harris R" first="Robert" last="Harris">Robert Harris</name></author><author><name sortKey="Church, John" sort="Church, John" uniqKey="Church J" first="John" last="Church">John Church</name></author><author><name sortKey="Brauner, Colin J" sort="Brauner, Colin J" uniqKey="Brauner C" first="Colin J" last="Brauner">Colin J. Brauner</name></author></analytic><series><title level="j">Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology</title><idno type="eISSN">1432-136X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acidosis (blood)</term><term>Acidosis (metabolism)</term><term>Animals</term><term>Carbon Dioxide (blood)</term><term>Carbon Dioxide (metabolism)</term><term>Cells, Cultured</term><term>Fishes (physiology)</term><term>Hepatocytes (cytology)</term><term>Hepatocytes (metabolism)</term><term>Hydrogen-Ion Concentration</term><term>Hypercapnia (blood)</term><term>Hypercapnia (metabolism)</term><term>Hypercapnia (pathology)</term><term>Intracellular Membranes (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Carbon Dioxide</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Acidosis</term><term>Hypercapnia</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hepatocytes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Acidosis</term><term>Carbon Dioxide</term><term>Hepatocytes</term><term>Hypercapnia</term><term>Intracellular Membranes</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Hypercapnia</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Hydrogen-Ion Concentration</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fish, exposed to elevated water CO(2), experience a rapid elevation in blood CO(2) (hypercapnia), resulting in acidification of both intra- and extra-cellular compartments. White sturgeon, Acipenser transmontanus, are exceptionally CO(2) tolerant and can regulate tissue intracellular pH (pH(i)) in the presence of a pronounced hypercapnic acidosis (preferential pH(i) regulation). In this study, pH(i) regulatory capacity of sturgeon liver cells in primary culture was examined to assess the suitability of employing this in vitro system to understand in vivo CO(2) tolerance in sturgeon. Using the pH-sensitive fluoroprobe BCECF, real-time changes in resting pH(i) and rates of pH(i) recovery were investigated during exposure to hypercapnia (3 and 6% CO(2)) in the absence and presence of additional acid loads induced by (20 mM) ammonium prepulse. During short-term (10 min) exposure to hypercapnia (3 and 6% CO(2)), sturgeon cells were acidified and no pH(i) compensation was observed. However, when exposure to 6% CO(2) was extended to over 19 h, the CO(2)-induced intracellular acidosis was partially compensated by a pH(i) increase of over 0.2 pH unit despite the sustained extracellular acidosis, indicative of a capacity for preferential pH(i) regulation in vitro. Since this capacity in sturgeon liver is present both in vivo and in vitro, the transmembrane transporters involved may be the same. Therefore, cell culture may be a suitable tool to identify the transporters (i.e., the cellular mechanisms underlying in vivo CO(2) tolerance) in white sturgeon and possibly in other hypercapnia-tolerant species.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Baker, Daniel W" sort="Baker, Daniel W" uniqKey="Baker D" first="Daniel W" last="Baker">Daniel W. Baker</name><name sortKey="Brauner, Colin J" sort="Brauner, Colin J" uniqKey="Brauner C" first="Colin J" last="Brauner">Colin J. Brauner</name><name sortKey="Church, John" sort="Church, John" uniqKey="Church J" first="John" last="Church">John Church</name><name sortKey="Harris, Robert" sort="Harris, Robert" uniqKey="Harris R" first="Robert" last="Harris">Robert Harris</name></noCountry><country name="Canada"><noRegion><name sortKey="Huynh, Khuong Tuyen" sort="Huynh, Khuong Tuyen" uniqKey="Huynh K" first="Khuong Tuyen" last="Huynh">Khuong Tuyen Huynh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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