Effects of Cryopreservation on Morphology and Viability of Sperm and Larvae of Atlantic Cod, Gadus morhua L.
Identifieur interne : 000521 ( Main/Exploration ); précédent : 000520; suivant : 000522Effects of Cryopreservation on Morphology and Viability of Sperm and Larvae of Atlantic Cod, Gadus morhua L.
Auteurs : Oddvar H. Ottesen [Norvège] ; Vegard Marschh User [Norvège] ; Igor Babiak [Norvège]Source :
- Journal of the World Aquaculture Society [ 0893-8849 ] ; 2012-06.
Abstract
The effects of cryopreservation on the viability, morphology and capability of spermatozoa in Atlantic cod, Gadus morhua L., were studied. The sperm was cryopreserved in straws using Hanks' balanced salt solution, hens' egg yolks and glycerol in the vapor of liquid nitrogen. Straws of cryopreserved sperm were stored in liquid nitrogen and thawed in seawater (35 C) for 8 sec before use. The motility of cryopreserved sperm was low (range 8–19%) compared to motility before freezing (range 69–76%). The fertilization rate (range 94–95%) in control groups using fresh sperm was significantly higher (P < 0.05) than in test groups (range 48–72%). In cryopreserved sperm, a relatively high percentage (range 82–93%) of the spermatozoa had changes in morphology. Many spermatozoa had no mitochondria; when mitochondria were present, the observed number varied from one and five in cryopreserved spermatozoa, and from two and seven in noncryopreserved spermatozoa. In groups where cryopreserved sperm was used, the hatching rate was lower (range 18–38%) than in control groups (range 41–63%), indicating higher mortality during embryonic development. Paternal effects on progeny performance were noted in the proportion of abnormalities but no negative effects were identified in newly hatched larvae produced using cryopreserved sperm.
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DOI: 10.1111/j.1749-7345.2012.00571.x
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Ottesen</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø</wicri:regionArea><wicri:noRegion>8049 Bodø</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Marschh User, Vegard" sort="Marschh User, Vegard" uniqKey="Marschh User V" first="Vegard" last="Marschh User">Vegard Marschh User</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø</wicri:regionArea><wicri:noRegion>8049 Bodø</wicri:noRegion></affiliation></author><author><name sortKey="Babiak, Igor" sort="Babiak, Igor" uniqKey="Babiak I" first="Igor" last="Babiak">Igor Babiak</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø</wicri:regionArea><wicri:noRegion>8049 Bodø</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of the World Aquaculture Society</title><idno type="ISSN">0893-8849</idno><idno type="eISSN">1749-7345</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06">2012-06</date><biblScope unit="volume">43</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="375">375</biblScope><biblScope unit="page" to="386">386</biblScope></imprint><idno type="ISSN">0893-8849</idno></series><idno type="istex">7A0F25D90350D0733E6D6B12CF807EF9B592D7D9</idno><idno type="DOI">10.1111/j.1749-7345.2012.00571.x</idno><idno type="ArticleID">JWAS571</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-8849</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The effects of cryopreservation on the viability, morphology and capability of spermatozoa in Atlantic cod, Gadus morhua L., were studied. The sperm was cryopreserved in straws using Hanks' balanced salt solution, hens' egg yolks and glycerol in the vapor of liquid nitrogen. Straws of cryopreserved sperm were stored in liquid nitrogen and thawed in seawater (35 C) for 8 sec before use. The motility of cryopreserved sperm was low (range 8–19%) compared to motility before freezing (range 69–76%). The fertilization rate (range 94–95%) in control groups using fresh sperm was significantly higher (P < 0.05) than in test groups (range 48–72%). In cryopreserved sperm, a relatively high percentage (range 82–93%) of the spermatozoa had changes in morphology. Many spermatozoa had no mitochondria; when mitochondria were present, the observed number varied from one and five in cryopreserved spermatozoa, and from two and seven in noncryopreserved spermatozoa. In groups where cryopreserved sperm was used, the hatching rate was lower (range 18–38%) than in control groups (range 41–63%), indicating higher mortality during embryonic development. Paternal effects on progeny performance were noted in the proportion of abnormalities but no negative effects were identified in newly hatched larvae produced using cryopreserved sperm.</div></front></TEI><affiliations><list><country><li>Norvège</li></country></list><tree><country name="Norvège"><noRegion><name sortKey="Ottesen, Oddvar H" sort="Ottesen, Oddvar H" uniqKey="Ottesen O" first="Oddvar H." last="Ottesen">Oddvar H. Ottesen</name></noRegion><name sortKey="Babiak, Igor" sort="Babiak, Igor" uniqKey="Babiak I" first="Igor" last="Babiak">Igor Babiak</name><name sortKey="Marschh User, Vegard" sort="Marschh User, Vegard" uniqKey="Marschh User V" first="Vegard" last="Marschh User">Vegard Marschh User</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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