cDNA Cloning and Functional Expression of Growth Hormone Receptor from Soft-Shelled Turtle ( Pelodiscus sinensis japonicus )
Identifieur interne : 001535 ( Main/Curation ); précédent : 001534; suivant : 001536cDNA Cloning and Functional Expression of Growth Hormone Receptor from Soft-Shelled Turtle ( Pelodiscus sinensis japonicus )
Auteurs : Xinna Zhang [République populaire de Chine] ; Xiongbin Lu [République populaire de Chine] ; Naihe Jing [République populaire de Chine] ; Shangquan Zhu [République populaire de Chine]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 2000.
English descriptors
- KwdEn :
Abstract
The growth hormone receptor (GHR) cDNA was cloned from the liver of soft-shelled turtle (Pelodiscus sinensis japonicus) using the polymerase chain reaction (PCR). Although GHR has been cloned from several mammalian and avian species, this is the first description of the reptilian receptor. As deduced from the nucleotide sequence, the precursor GHR of soft-shelled turtle (tGHR) is a protein of 615 amino acids which presents 72% identity with the chicken receptor and 57–64% identity with GHRs of several mammals. The tGHR expressed in COS-7 cells specifically bound human growth hormone (hGH) and was able to transduce an activation of transcription in the transfected cells. Binding of 125I-hGH to the expressed receptor was decreased by the addition of excess unlabeled hGH, pig GH, and bream GH but not by pig insulin. The open reading frame of tGHR cDNA was inserted into the pSINrep/gfp (green fluorescence protein) vector and the tGHR-gfp fusion protein was stably expressed in baby hamster kidney (BHK) cells. Confocal imaging showed that tGHR-gfp was largely concentrated on the plasma membrane. Western blot analysis and deglycosylation treatment with PNGase F demonstrated that tGHR was a glycoprotein in BHK cells.
Url:
DOI: 10.1006/gcen.2000.7530
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000D66
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000D64
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000C61
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001612
Links to Exploration step
ISTEX:83786B4492B424A9727478AADE1E2A0492C0308DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">cDNA Cloning and Functional Expression of Growth Hormone Receptor from Soft-Shelled Turtle ( Pelodiscus sinensis japonicus )</title>
<author><name sortKey="Zhang, Xinna" sort="Zhang, Xinna" uniqKey="Zhang X" first="Xinna" last="Zhang">Xinna Zhang</name>
</author>
<author><name sortKey="Lu, Xiongbin" sort="Lu, Xiongbin" uniqKey="Lu X" first="Xiongbin" last="Lu">Xiongbin Lu</name>
</author>
<author><name sortKey="Jing, Naihe" sort="Jing, Naihe" uniqKey="Jing N" first="Naihe" last="Jing">Naihe Jing</name>
</author>
<author><name sortKey="Zhu, Shangquan" sort="Zhu, Shangquan" uniqKey="Zhu S" first="Shangquan" last="Zhu">Shangquan Zhu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:83786B4492B424A9727478AADE1E2A0492C0308D</idno>
<date when="2000" year="2000">2000</date>
<idno type="doi">10.1006/gcen.2000.7530</idno>
<idno type="url">https://api.istex.fr/document/83786B4492B424A9727478AADE1E2A0492C0308D/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D66</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D66</idno>
<idno type="wicri:Area/Istex/Curation">000D64</idno>
<idno type="wicri:Area/Istex/Checkpoint">000C61</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C61</idno>
<idno type="wicri:doubleKey">0016-6480:2000:Zhang X:cdna:cloning:and</idno>
<idno type="wicri:Area/Main/Merge">001612</idno>
<idno type="wicri:Area/Main/Curation">001535</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">cDNA Cloning and Functional Expression of Growth Hormone Receptor from Soft-Shelled Turtle ( Pelodiscus sinensis japonicus )</title>
<author><name sortKey="Zhang, Xinna" sort="Zhang, Xinna" uniqKey="Zhang X" first="Xinna" last="Zhang">Xinna Zhang</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, 200031</wicri:regionArea>
<wicri:noRegion>200031</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lu, Xiongbin" sort="Lu, Xiongbin" uniqKey="Lu X" first="Xiongbin" last="Lu">Xiongbin Lu</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, 200031</wicri:regionArea>
<wicri:noRegion>200031</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jing, Naihe" sort="Jing, Naihe" uniqKey="Jing N" first="Naihe" last="Jing">Naihe Jing</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, 200031</wicri:regionArea>
<wicri:noRegion>200031</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhu, Shangquan" sort="Zhu, Shangquan" uniqKey="Zhu S" first="Shangquan" last="Zhu">Shangquan Zhu</name>
<affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, 200031</wicri:regionArea>
<wicri:noRegion>200031</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">General and Comparative Endocrinology</title>
<title level="j" type="abbrev">YGCEN</title>
<idno type="ISSN">0016-6480</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="2000">2000</date>
<biblScope unit="volume">119</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="265">265</biblScope>
<biblScope unit="page" to="275">275</biblScope>
</imprint>
<idno type="ISSN">0016-6480</idno>
</series>
<idno type="istex">83786B4492B424A9727478AADE1E2A0492C0308D</idno>
<idno type="DOI">10.1006/gcen.2000.7530</idno>
<idno type="PII">S0016-6480(00)97530-0</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0016-6480</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>BHK cells</term>
<term>COS-7 cells</term>
<term>cDNA</term>
<term>expression</term>
<term>growth hormone receptor (GHR)</term>
<term>sequence</term>
<term>soft-shelled turtle</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The growth hormone receptor (GHR) cDNA was cloned from the liver of soft-shelled turtle (Pelodiscus sinensis japonicus) using the polymerase chain reaction (PCR). Although GHR has been cloned from several mammalian and avian species, this is the first description of the reptilian receptor. As deduced from the nucleotide sequence, the precursor GHR of soft-shelled turtle (tGHR) is a protein of 615 amino acids which presents 72% identity with the chicken receptor and 57–64% identity with GHRs of several mammals. The tGHR expressed in COS-7 cells specifically bound human growth hormone (hGH) and was able to transduce an activation of transcription in the transfected cells. Binding of 125I-hGH to the expressed receptor was decreased by the addition of excess unlabeled hGH, pig GH, and bream GH but not by pig insulin. The open reading frame of tGHR cDNA was inserted into the pSINrep/gfp (green fluorescence protein) vector and the tGHR-gfp fusion protein was stably expressed in baby hamster kidney (BHK) cells. Confocal imaging showed that tGHR-gfp was largely concentrated on the plasma membrane. Western blot analysis and deglycosylation treatment with PNGase F demonstrated that tGHR was a glycoprotein in BHK cells.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001535 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001535 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:83786B4492B424A9727478AADE1E2A0492C0308D |texte= cDNA Cloning and Functional Expression of Growth Hormone Receptor from Soft-Shelled Turtle ( Pelodiscus sinensis japonicus ) }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |