Tissue specificity of aryl hydrocarbon receptor (AhR) mediated responses and relative sensitivity of white sturgeon (Acipenser transmontanus) to an AhR agonist.
Identifieur interne : 000424 ( Main/Curation ); précédent : 000423; suivant : 000425Tissue specificity of aryl hydrocarbon receptor (AhR) mediated responses and relative sensitivity of white sturgeon (Acipenser transmontanus) to an AhR agonist.
Auteurs : Jon A. Doering [Canada] ; Steve Wiseman ; Shawn C. Beitel ; Brett J. Tendler ; John P. Giesy ; Markus HeckerSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 1879-1514 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Aryl Hydrocarbon Hydroxylases (genetics), Aryl Hydrocarbon Hydroxylases (metabolism), Dose-Response Relationship, Drug, Enzyme Inhibitors (pharmacology), Fishes, Gene Expression Regulation, Gills (metabolism), Intestines (metabolism), Liver (metabolism), Phylogeny, Receptors, Aryl Hydrocarbon (agonists), Receptors, Aryl Hydrocarbon (genetics), Receptors, Aryl Hydrocarbon (metabolism), beta-Naphthoflavone (pharmacology).
- MESH :
- chemical , agonists : Receptors, Aryl Hydrocarbon.
- chemical , genetics : Aryl Hydrocarbon Hydroxylases, Receptors, Aryl Hydrocarbon.
- chemical , metabolism : Aryl Hydrocarbon Hydroxylases, Receptors, Aryl Hydrocarbon.
- chemical , pharmacology : Enzyme Inhibitors, beta-Naphthoflavone.
- metabolism : Gills, Intestines, Liver.
- Animals, Dose-Response Relationship, Drug, Fishes, Gene Expression Regulation, Phylogeny.
Abstract
Sturgeons are endangered in some parts of the world. Due to their benthic nature and longevity sturgeon are at greater risk of exposure to bioaccumulative contaminants such as dioxin-like compounds that are associated with sediments. Despite their endangered status, little research has been conducted to characterize the relative responsiveness of sturgeon to dioxin-like compounds. In an attempt to study the biological effects and possible associated risks of exposure to dioxin-like compounds in sturgeon, the molecular and biochemical responses of white sturgeon (Acipenser transmontanus) to a model aryl hydrocarbon receptor (AhR) agonist, β-naphthoflavone (βNF) were investigated. White sturgeon were injected intraperitoneally with one of three doses of βNF (0, 50, or 500mg/kg, bw). Rainbow trout (Oncorhynchus mykiss) were used as a reference species since their responses have been well characterized in the past. Three days following injection with βNF, fish were euthanized and livers, gills, and intestines collected for biochemical and molecular analyses. White sturgeon exposed to βNF had significantly greater ethoxyresorufin O-deethylase (EROD) activity in liver (up to 37-fold), gill (up to 41-fold), and intestine (up to 36-fold) than did unexposed controls. Rainbow trout injected with βNF exhibited EROD activity that was significantly greater in liver (88-fold), than that of controls, but was undetectable in gills or intestine. Abundance of CYP1A transcript displayed a comparable pattern of tissue-specific induction with intestine (up to 189-fold), gills (up to 53-fold), and liver (up to 21-fold). Methoxyresorufin O-deethylase (MROD) and pentoxyresorufin O-deethylase (PROD) activities were undetectable in unexposed white sturgeon tissues while exposed tissues displayed MROD activity that was only moderately greater than the activity that could be detected. Differential inducibility among liver, gill, and intestine following exposure to an AhR agonist is likely associated with tissue-specific regulation of the AhR signalling pathway. Liver and gill of white sturgeon had significantly greater AhR transcript abundance than did the intestine, however following exposure to βNF, significantly greater induction in AhR transcript abundance was detected in intestine (up to 35-fold) compared to liver (up to 5-fold) or gills (up to 11-fold). It was shown that white sturgeon are responsive to AhR agonists in the liver, gill, and intestine and could be among the more sensitive fish species with regard to inducibility of CYP1A.
DOI: 10.1016/j.aquatox.2012.02.015
PubMed: 22446824
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000309
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000309
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000309
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000424
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000424
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000424
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :000430
Links to Exploration step
pubmed:22446824Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tissue specificity of aryl hydrocarbon receptor (AhR) mediated responses and relative sensitivity of white sturgeon (Acipenser transmontanus) to an AhR agonist.</title>
<author><name sortKey="Doering, Jon A" sort="Doering, Jon A" uniqKey="Doering J" first="Jon A" last="Doering">Jon A. Doering</name>
<affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK S7N5B3, Canada. jad929@mail.usask.ca</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK S7N5B3</wicri:regionArea>
<wicri:noRegion>SK S7N5B3</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wiseman, Steve" sort="Wiseman, Steve" uniqKey="Wiseman S" first="Steve" last="Wiseman">Steve Wiseman</name>
</author>
<author><name sortKey="Beitel, Shawn C" sort="Beitel, Shawn C" uniqKey="Beitel S" first="Shawn C" last="Beitel">Shawn C. Beitel</name>
</author>
<author><name sortKey="Tendler, Brett J" sort="Tendler, Brett J" uniqKey="Tendler B" first="Brett J" last="Tendler">Brett J. Tendler</name>
</author>
<author><name sortKey="Giesy, John P" sort="Giesy, John P" uniqKey="Giesy J" first="John P" last="Giesy">John P. Giesy</name>
</author>
<author><name sortKey="Hecker, Markus" sort="Hecker, Markus" uniqKey="Hecker M" first="Markus" last="Hecker">Markus Hecker</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2012">2012</date>
<idno type="RBID">pubmed:22446824</idno>
<idno type="pmid">22446824</idno>
<idno type="doi">10.1016/j.aquatox.2012.02.015</idno>
<idno type="wicri:Area/PubMed/Corpus">000309</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000309</idno>
<idno type="wicri:Area/PubMed/Curation">000309</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000309</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000309</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000309</idno>
<idno type="wicri:Area/Ncbi/Merge">000424</idno>
<idno type="wicri:Area/Ncbi/Curation">000424</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000424</idno>
<idno type="wicri:Area/Main/Merge">000430</idno>
<idno type="wicri:Area/Main/Curation">000424</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Tissue specificity of aryl hydrocarbon receptor (AhR) mediated responses and relative sensitivity of white sturgeon (Acipenser transmontanus) to an AhR agonist.</title>
<author><name sortKey="Doering, Jon A" sort="Doering, Jon A" uniqKey="Doering J" first="Jon A" last="Doering">Jon A. Doering</name>
<affiliation wicri:level="1"><nlm:affiliation>Toxicology Centre, University of Saskatchewan, Saskatoon, SK S7N5B3, Canada. jad929@mail.usask.ca</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Toxicology Centre, University of Saskatchewan, Saskatoon, SK S7N5B3</wicri:regionArea>
<wicri:noRegion>SK S7N5B3</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wiseman, Steve" sort="Wiseman, Steve" uniqKey="Wiseman S" first="Steve" last="Wiseman">Steve Wiseman</name>
</author>
<author><name sortKey="Beitel, Shawn C" sort="Beitel, Shawn C" uniqKey="Beitel S" first="Shawn C" last="Beitel">Shawn C. Beitel</name>
</author>
<author><name sortKey="Tendler, Brett J" sort="Tendler, Brett J" uniqKey="Tendler B" first="Brett J" last="Tendler">Brett J. Tendler</name>
</author>
<author><name sortKey="Giesy, John P" sort="Giesy, John P" uniqKey="Giesy J" first="John P" last="Giesy">John P. Giesy</name>
</author>
<author><name sortKey="Hecker, Markus" sort="Hecker, Markus" uniqKey="Hecker M" first="Markus" last="Hecker">Markus Hecker</name>
</author>
</analytic>
<series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title>
<idno type="eISSN">1879-1514</idno>
<imprint><date when="2012" type="published">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Aryl Hydrocarbon Hydroxylases (genetics)</term>
<term>Aryl Hydrocarbon Hydroxylases (metabolism)</term>
<term>Dose-Response Relationship, Drug</term>
<term>Enzyme Inhibitors (pharmacology)</term>
<term>Fishes</term>
<term>Gene Expression Regulation</term>
<term>Gills (metabolism)</term>
<term>Intestines (metabolism)</term>
<term>Liver (metabolism)</term>
<term>Phylogeny</term>
<term>Receptors, Aryl Hydrocarbon (agonists)</term>
<term>Receptors, Aryl Hydrocarbon (genetics)</term>
<term>Receptors, Aryl Hydrocarbon (metabolism)</term>
<term>beta-Naphthoflavone (pharmacology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="agonists" xml:lang="en"><term>Receptors, Aryl Hydrocarbon</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aryl Hydrocarbon Hydroxylases</term>
<term>Receptors, Aryl Hydrocarbon</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aryl Hydrocarbon Hydroxylases</term>
<term>Receptors, Aryl Hydrocarbon</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term>
<term>beta-Naphthoflavone</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gills</term>
<term>Intestines</term>
<term>Liver</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Dose-Response Relationship, Drug</term>
<term>Fishes</term>
<term>Gene Expression Regulation</term>
<term>Phylogeny</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Sturgeons are endangered in some parts of the world. Due to their benthic nature and longevity sturgeon are at greater risk of exposure to bioaccumulative contaminants such as dioxin-like compounds that are associated with sediments. Despite their endangered status, little research has been conducted to characterize the relative responsiveness of sturgeon to dioxin-like compounds. In an attempt to study the biological effects and possible associated risks of exposure to dioxin-like compounds in sturgeon, the molecular and biochemical responses of white sturgeon (Acipenser transmontanus) to a model aryl hydrocarbon receptor (AhR) agonist, β-naphthoflavone (βNF) were investigated. White sturgeon were injected intraperitoneally with one of three doses of βNF (0, 50, or 500mg/kg, bw). Rainbow trout (Oncorhynchus mykiss) were used as a reference species since their responses have been well characterized in the past. Three days following injection with βNF, fish were euthanized and livers, gills, and intestines collected for biochemical and molecular analyses. White sturgeon exposed to βNF had significantly greater ethoxyresorufin O-deethylase (EROD) activity in liver (up to 37-fold), gill (up to 41-fold), and intestine (up to 36-fold) than did unexposed controls. Rainbow trout injected with βNF exhibited EROD activity that was significantly greater in liver (88-fold), than that of controls, but was undetectable in gills or intestine. Abundance of CYP1A transcript displayed a comparable pattern of tissue-specific induction with intestine (up to 189-fold), gills (up to 53-fold), and liver (up to 21-fold). Methoxyresorufin O-deethylase (MROD) and pentoxyresorufin O-deethylase (PROD) activities were undetectable in unexposed white sturgeon tissues while exposed tissues displayed MROD activity that was only moderately greater than the activity that could be detected. Differential inducibility among liver, gill, and intestine following exposure to an AhR agonist is likely associated with tissue-specific regulation of the AhR signalling pathway. Liver and gill of white sturgeon had significantly greater AhR transcript abundance than did the intestine, however following exposure to βNF, significantly greater induction in AhR transcript abundance was detected in intestine (up to 35-fold) compared to liver (up to 5-fold) or gills (up to 11-fold). It was shown that white sturgeon are responsive to AhR agonists in the liver, gill, and intestine and could be among the more sensitive fish species with regard to inducibility of CYP1A.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000424 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000424 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:22446824 |texte= Tissue specificity of aryl hydrocarbon receptor (AhR) mediated responses and relative sensitivity of white sturgeon (Acipenser transmontanus) to an AhR agonist. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:22446824" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
This area was generated with Dilib version V0.6.27. |