Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays
Identifieur interne : 001307 ( Istex/Curation ); précédent : 001306; suivant : 001308Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays
Auteurs : Assia Aouadene [France] ; Carole Di Giorgio [France] ; Luc Sarrazin [France] ; Xavier Moreau [France] ; Laetitia De Jong [France] ; Fabrice Garcia [France] ; Alain Thiery [France] ; Alain Botta [France] ; Michel De Méo [France]Source :
- Environmental and Molecular Mutagenesis [ 0893-6692 ] ; 2008-05.
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Abstract
The present investigation evaluated the capacity of the Salmonella mutagenicity test, the comet assay, and the micronucleus assay to detect and characterize the genotoxic profile of river sediments. Three stations were selected on an urban river (Bouches du Rhône, France) exposed to various sources of industrial and urban pollution (StA, StB, and StC) and one station on its tributary (StD). One station in a nonurban river was included (REF). The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined by HPLC, and the genotoxicity of the sediments was monitored by the Salmonella mutagenicity test (TA98 + S9, YG1041 ± S9), the comet assay, and the micronucleus assay on CHO cells. Chemical analysis showed that the total PAH concentrations ranged from 23 μg kg−1 dw (REF) to 1285 μg kg−1 dw (StD). All the sediments were mutagenic in the Salmonella mutagenicity test. The mutagenicity was probably induced by the presence of nitroarenes (StA, StB, StC, and StD) and aromatic amines (REF) as deduced from the mutagenicity profiles of strains YG1041 ± S9 and TA98 + S9. The comet assay revealed direct DNA lesions in REF, StA, and StB sediments and metabolization‐dependent DNA damage in StC and StD. The micronucleus assay showed an absence of clastogenicity for StA ± S9 and StC‐S9, and a significant clastogenicity ± S9 for the three other stations. The genotoxicity ranking determined by the comet assay + S9 matched the ranking of total and carcinogenic PAH concentrations, and this assay was found to be the most sensitive. Environ. Mol. Mutagen., 2008. © 2008 Wiley‐Liss, Inc.
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DOI: 10.1002/em.20384
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Faculté de Pharmacie, Marseille, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Hydrologie et de Molysmologie Aquatique, Université de la Méditerranée, Faculté de Pharmacie, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Moreau, Xavier" sort="Moreau, Xavier" uniqKey="Moreau X" first="Xavier" last="Moreau">Xavier Moreau</name><affiliation wicri:level="1"><mods:affiliation>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="De Jong, Laetitia" sort="De Jong, Laetitia" uniqKey="De Jong L" first="Laetitia" last="De Jong">Laetitia De Jong</name><affiliation wicri:level="1"><mods:affiliation>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR‐Centre National de la Recherche Scientifique 6116 IMEP, Case 17, Université de Provence, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Garcia, Fabrice" sort="Garcia, Fabrice" uniqKey="Garcia F" first="Fabrice" last="Garcia">Fabrice Garcia</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire d'Hydrologie et de Molysmologie Aquatique, Université de la Méditerranée, Faculté de Pharmacie, Marseille, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Hydrologie et de Molysmologie Aquatique, Université de la Méditerranée, Faculté de Pharmacie, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Thiery, Alain" sort="Thiery, Alain" uniqKey="Thiery A" first="Alain" last="Thiery">Alain Thiery</name><affiliation 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uniqKey="De Meo M" first="Michel" last="De Méo">Michel De Méo</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Université de la Méditerranée, Facultés de Médecine et Pharmacie, Marseille, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Université de la Méditerranée, Facultés de Médecine et Pharmacie, Marseille</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, EA 1784, Faculté de Pharmacie, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, EA 1784, Faculté de Pharmacie, 27 Bd Jean Moulin, 13385 Marseille Cedex 05</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental and Molecular Mutagenesis</title><title level="j" type="abbrev">Environ. Mol. Mutagen.</title><idno type="ISSN">0893-6692</idno><idno type="eISSN">1098-2280</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2008-05">2008-05</date><biblScope unit="volume">49</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="283">283</biblScope><biblScope unit="page" to="299">299</biblScope></imprint><idno type="ISSN">0893-6692</idno></series><idno type="istex">0A7349E858A1896C845739DD768E1F034CA0D4D1</idno><idno type="DOI">10.1002/em.20384</idno><idno type="ArticleID">EM20384</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-6692</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>PAHs</term><term>Salmonella mutagenicity test</term><term>comet assay</term><term>genotoxicity</term><term>micronucleus assay</term><term>pollution</term><term>river</term><term>sediment</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present investigation evaluated the capacity of the Salmonella mutagenicity test, the comet assay, and the micronucleus assay to detect and characterize the genotoxic profile of river sediments. Three stations were selected on an urban river (Bouches du Rhône, France) exposed to various sources of industrial and urban pollution (StA, StB, and StC) and one station on its tributary (StD). One station in a nonurban river was included (REF). The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined by HPLC, and the genotoxicity of the sediments was monitored by the Salmonella mutagenicity test (TA98 + S9, YG1041 ± S9), the comet assay, and the micronucleus assay on CHO cells. Chemical analysis showed that the total PAH concentrations ranged from 23 μg kg−1 dw (REF) to 1285 μg kg−1 dw (StD). All the sediments were mutagenic in the Salmonella mutagenicity test. The mutagenicity was probably induced by the presence of nitroarenes (StA, StB, StC, and StD) and aromatic amines (REF) as deduced from the mutagenicity profiles of strains YG1041 ± S9 and TA98 + S9. The comet assay revealed direct DNA lesions in REF, StA, and StB sediments and metabolization‐dependent DNA damage in StC and StD. The micronucleus assay showed an absence of clastogenicity for StA ± S9 and StC‐S9, and a significant clastogenicity ± S9 for the three other stations. The genotoxicity ranking determined by the comet assay + S9 matched the ranking of total and carcinogenic PAH concentrations, and this assay was found to be the most sensitive. Environ. Mol. Mutagen., 2008. © 2008 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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