Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA
Identifieur interne : 000E86 ( Istex/Curation ); précédent : 000E85; suivant : 000E87Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA
Auteurs : Robert M. Dores ; Tana R. Smith ; David A. Rubin [États-Unis] ; Phillip Danielson ; Luciano E. Marra [Canada] ; John H. Youson [Canada]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1997.
Abstract
A cDNA that codes for the polypeptide hormone precursor proopiomelanocortin (POMC) was cloned and sequenced from a gar (Lepisosteus osseus) pituitary cDNA library. The gar POMC cDNA is 1237 bp and contains a 780-bp open reading frame. The deduced amino acid sequence for gar POMC is 259 amino acids in length. The general organization of gar POMC is very similar to that of other gnathostome POMC sequences. The β-endorphin sequence had 91% sequence identity with sockeye A β-endorphin and 71% sequence identity withXenopus laevisβ-endorphin. Three melanocyte-stimulating hormone (MSH) core sequences [HFR(W)] were detected. The gar α-MSH sequence was identical to the α-MSH sequence in rat POMC. The gar β-MSH sequence had 77% sequence identity with salmonid forms of β-MSH and 53% sequence identity with tetrapod forms of β-MSH. The γ-MSH region of gar POMC only had 26% primary sequence identity with tetrapod γ-MSH sequences. Gar γ-MSH had an incomplete MSH core sequence (HRF), an apparent internal deletion of five amino acids, and lacked flanking paired basic amino acids essential for proteolytic cleavage. The apparent degenerate nature of gar γ-MSH is discussed in light of the absence of this sequence in salmonid fish.
Url:
DOI: 10.1006/gcen.1997.6947
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000E88
Links to Exploration step
ISTEX:02BD825C481E7FD86032FD519FE32ED3C5D61AE1Curation
No country items
Robert M. Dores<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA</title>
<author><name sortKey="Dores, Robert M" sort="Dores, Robert M" uniqKey="Dores R" first="Robert M." last="Dores">Robert M. Dores</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Smith, Tana R" sort="Smith, Tana R" uniqKey="Smith T" first="Tana R." last="Smith">Tana R. Smith</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Rubin, David A" sort="Rubin, David A" uniqKey="Rubin D" first="David A." last="Rubin">David A. Rubin</name>
<affiliation wicri:level="2"><mods:affiliation>Endocrine Unit, Massachusetts General Hospital, Harvard University Medical School, Boston, Massachusetts</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Endocrine Unit, Massachusetts General Hospital, Harvard University Medical School, Boston</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Danielson, Phillip" sort="Danielson, Phillip" uniqKey="Danielson P" first="Phillip" last="Danielson">Phillip Danielson</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Marra, Luciano E" sort="Marra, Luciano E" uniqKey="Marra L" first="Luciano E." last="Marra">Luciano E. Marra</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Youson, John H" sort="Youson, John H" uniqKey="Youson J" first="John H." last="Youson">John H. Youson</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:02BD825C481E7FD86032FD519FE32ED3C5D61AE1</idno>
<date when="1997" year="1997">1997</date>
<idno type="doi">10.1006/gcen.1997.6947</idno>
<idno type="url">https://api.istex.fr/document/02BD825C481E7FD86032FD519FE32ED3C5D61AE1/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000E88</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E88</idno>
<idno type="wicri:Area/Istex/Curation">000E86</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA</title>
<author><name sortKey="Dores, Robert M" sort="Dores, Robert M" uniqKey="Dores R" first="Robert M." last="Dores">Robert M. Dores</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Smith, Tana R" sort="Smith, Tana R" uniqKey="Smith T" first="Tana R." last="Smith">Tana R. Smith</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Rubin, David A" sort="Rubin, David A" uniqKey="Rubin D" first="David A." last="Rubin">David A. Rubin</name>
<affiliation wicri:level="2"><mods:affiliation>Endocrine Unit, Massachusetts General Hospital, Harvard University Medical School, Boston, Massachusetts</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Endocrine Unit, Massachusetts General Hospital, Harvard University Medical School, Boston</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Danielson, Phillip" sort="Danielson, Phillip" uniqKey="Danielson P" first="Phillip" last="Danielson">Phillip Danielson</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation>
<wicri:noCountry code="subField">80208</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Marra, Luciano E" sort="Marra, Luciano E" uniqKey="Marra L" first="Luciano E." last="Marra">Luciano E. Marra</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Youson, John H" sort="Youson, John H" uniqKey="Youson J" first="John H." last="Youson">John H. Youson</name>
<affiliation wicri:level="1"><mods:affiliation>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Division of Life Sciences, University of Toronto, Scarborough Campus, Scarborough, Ontario, M1C 1A4</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">General and Comparative Endocrinology</title>
<title level="j" type="abbrev">YGCEN</title>
<idno type="ISSN">0016-6480</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1997">1997</date>
<biblScope unit="volume">107</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="401">401</biblScope>
<biblScope unit="page" to="413">413</biblScope>
</imprint>
<idno type="ISSN">0016-6480</idno>
</series>
<idno type="istex">02BD825C481E7FD86032FD519FE32ED3C5D61AE1</idno>
<idno type="DOI">10.1006/gcen.1997.6947</idno>
<idno type="PII">S0016-6480(97)96947-1</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0016-6480</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A cDNA that codes for the polypeptide hormone precursor proopiomelanocortin (POMC) was cloned and sequenced from a gar (Lepisosteus osseus) pituitary cDNA library. The gar POMC cDNA is 1237 bp and contains a 780-bp open reading frame. The deduced amino acid sequence for gar POMC is 259 amino acids in length. The general organization of gar POMC is very similar to that of other gnathostome POMC sequences. The β-endorphin sequence had 91% sequence identity with sockeye A β-endorphin and 71% sequence identity withXenopus laevisβ-endorphin. Three melanocyte-stimulating hormone (MSH) core sequences [HFR(W)] were detected. The gar α-MSH sequence was identical to the α-MSH sequence in rat POMC. The gar β-MSH sequence had 77% sequence identity with salmonid forms of β-MSH and 53% sequence identity with tetrapod forms of β-MSH. The γ-MSH region of gar POMC only had 26% primary sequence identity with tetrapod γ-MSH sequences. Gar γ-MSH had an incomplete MSH core sequence (HRF), an apparent internal deletion of five amino acids, and lacked flanking paired basic amino acids essential for proteolytic cleavage. The apparent degenerate nature of gar γ-MSH is discussed in light of the absence of this sequence in salmonid fish.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E86 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000E86 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:02BD825C481E7FD86032FD519FE32ED3C5D61AE1 |texte= Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |