Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA
Identifieur interne : 000E86 ( Istex/Curation ); précédent : 000E85; suivant : 000E87Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA
Auteurs : Robert M. Dores ; Tana R. Smith ; David A. Rubin [États-Unis] ; Phillip Danielson ; Luciano E. Marra [Canada] ; John H. Youson [Canada]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1997.
Abstract
A cDNA that codes for the polypeptide hormone precursor proopiomelanocortin (POMC) was cloned and sequenced from a gar (Lepisosteus osseus) pituitary cDNA library. The gar POMC cDNA is 1237 bp and contains a 780-bp open reading frame. The deduced amino acid sequence for gar POMC is 259 amino acids in length. The general organization of gar POMC is very similar to that of other gnathostome POMC sequences. The β-endorphin sequence had 91% sequence identity with sockeye A β-endorphin and 71% sequence identity withXenopus laevisβ-endorphin. Three melanocyte-stimulating hormone (MSH) core sequences [HFR(W)] were detected. The gar α-MSH sequence was identical to the α-MSH sequence in rat POMC. The gar β-MSH sequence had 77% sequence identity with salmonid forms of β-MSH and 53% sequence identity with tetrapod forms of β-MSH. The γ-MSH region of gar POMC only had 26% primary sequence identity with tetrapod γ-MSH sequences. Gar γ-MSH had an incomplete MSH core sequence (HRF), an apparent internal deletion of five amino acids, and lacked flanking paired basic amino acids essential for proteolytic cleavage. The apparent degenerate nature of gar γ-MSH is discussed in light of the absence of this sequence in salmonid fish.
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DOI: 10.1006/gcen.1997.6947
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Robert M. Dores<affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation><wicri:noCountry code="subField">80208</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation><wicri:noCountry code="subField">80208</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Biological Sciences, University of Denver, Denver, Colorado, 80208</mods:affiliation><wicri:noCountry code="subField">80208</wicri:noCountry></affiliation>Le document en format XML
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The gar POMC cDNA is 1237 bp and contains a 780-bp open reading frame. The deduced amino acid sequence for gar POMC is 259 amino acids in length. The general organization of gar POMC is very similar to that of other gnathostome POMC sequences. The β-endorphin sequence had 91% sequence identity with sockeye A β-endorphin and 71% sequence identity withXenopus laevisβ-endorphin. Three melanocyte-stimulating hormone (MSH) core sequences [HFR(W)] were detected. The gar α-MSH sequence was identical to the α-MSH sequence in rat POMC. The gar β-MSH sequence had 77% sequence identity with salmonid forms of β-MSH and 53% sequence identity with tetrapod forms of β-MSH. The γ-MSH region of gar POMC only had 26% primary sequence identity with tetrapod γ-MSH sequences. Gar γ-MSH had an incomplete MSH core sequence (HRF), an apparent internal deletion of five amino acids, and lacked flanking paired basic amino acids essential for proteolytic cleavage. The apparent degenerate nature of gar γ-MSH is discussed in light of the absence of this sequence in salmonid fish.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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