Cytochrome b of fish mitochondria is strongly resistant to funiculosin, a powerful inhibitor of respiration
Identifieur interne : 000A20 ( Istex/Curation ); précédent : 000A19; suivant : 000A21Cytochrome b of fish mitochondria is strongly resistant to funiculosin, a powerful inhibitor of respiration
Auteurs : Mauro Degli Esposti [États-Unis] ; Anna Ghelli [Italie] ; Massimo Crimi [États-Unis] ; Alessandra Baracca [États-Unis] ; Giancarlo Solaini [Italie] ; Thierry Tron [États-Unis] ; Axel Meyer [États-Unis]Source :
- Archives of Biochemistry and Biophysics [ 0003-9861 ] ; 1992.
Abstract
We report here some unusual properties of ubiquinol: cytochrome c reductase of eel and other fish mitochondria. The turnover rate of the reductase is clearly higher than in mammalian mitochondria and the binding constant for ubiquinone seems to be larger than in other vertebrates. Additionally, the reductase activity of fish mitochondria is resistant to some powerful inhibitors that bind to cytochrome b, in particular to funiculosin. After sequencing most of the gene of eel cytochrome b and comparing the deduced amino acid sequence with that of other fish and animals, we hypothesize that the decreased binding of funiculosin could be due to a few amino acid replacements in the third and fourth transmembrane helix of the protein. In particular, the presence of methionine instead of alanine at position 125 seems to be largely responsible for the strong resistance to funiculosin and also to the partial resistance to myxothiazol in all fish mitochondria. Correlations between some residue substitutions in cytochrome b and the different effects of funiculosin in different species are also considered.
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DOI: 10.1016/0003-9861(92)90506-R
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Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. 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Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Meyer, Axel" sort="Meyer, Axel" uniqKey="Meyer A" first="Axel" last="Meyer">Axel Meyer</name><affiliation wicri:level="2"><mods:affiliation>Department of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A2484CB35239548DD4EB34F32FFF902C517A7480</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1016/0003-9861(92)90506-R</idno><idno type="url">https://api.istex.fr/document/A2484CB35239548DD4EB34F32FFF902C517A7480/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A22</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A22</idno><idno type="wicri:Area/Istex/Curation">000A20</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Cytochrome b of fish mitochondria is strongly resistant to funiculosin, a powerful inhibitor of respiration</title><author><name sortKey="Esposti, Mauro Degli" sort="Esposti, Mauro Degli" uniqKey="Esposti M" first="Mauro Degli" last="Esposti">Mauro Degli Esposti</name><affiliation wicri:level="2"><mods:affiliation>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Ghelli, Anna" sort="Ghelli, Anna" uniqKey="Ghelli A" first="Anna" last="Ghelli">Anna Ghelli</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy</mods:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna</wicri:regionArea></affiliation></author><author><name sortKey="Crimi, Massimo" sort="Crimi, Massimo" uniqKey="Crimi M" first="Massimo" last="Crimi">Massimo Crimi</name><affiliation wicri:level="2"><mods:affiliation>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Baracca, Alessandra" sort="Baracca, Alessandra" uniqKey="Baracca A" first="Alessandra" last="Baracca">Alessandra Baracca</name><affiliation wicri:level="2"><mods:affiliation>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Solaini, Giancarlo" sort="Solaini, Giancarlo" uniqKey="Solaini G" first="Giancarlo" last="Solaini">Giancarlo Solaini</name><affiliation wicri:level="1"><mods:affiliation>Scuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, Italy</mods:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Scuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa</wicri:regionArea></affiliation></author><author><name sortKey="Tron, Thierry" sort="Tron, Thierry" uniqKey="Tron T" first="Thierry" last="Tron">Thierry Tron</name><affiliation wicri:level="2"><mods:affiliation>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Biophysics, Department of Biology, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyScuola Superiore di Studi Universitari e Perfezionamento S. Anna, Pisa, ItalyDepartment of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Meyer, Axel" sort="Meyer, Axel" uniqKey="Meyer A" first="Axel" last="Meyer">Axel Meyer</name><affiliation wicri:level="2"><mods:affiliation>Department of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-5245 USA</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Ecology and Evolution, Division of Biological Sciences, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Biochemistry and Biophysics</title><title level="j" type="abbrev">YABBI</title><idno type="ISSN">0003-9861</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">295</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="198">198</biblScope><biblScope unit="page" to="204">204</biblScope></imprint><idno type="ISSN">0003-9861</idno></series><idno type="istex">A2484CB35239548DD4EB34F32FFF902C517A7480</idno><idno type="DOI">10.1016/0003-9861(92)90506-R</idno><idno type="PII">0003-9861(92)90506-R</idno><idno type="ArticleID">9290506R</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-9861</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report here some unusual properties of ubiquinol: cytochrome c reductase of eel and other fish mitochondria. The turnover rate of the reductase is clearly higher than in mammalian mitochondria and the binding constant for ubiquinone seems to be larger than in other vertebrates. Additionally, the reductase activity of fish mitochondria is resistant to some powerful inhibitors that bind to cytochrome b, in particular to funiculosin. After sequencing most of the gene of eel cytochrome b and comparing the deduced amino acid sequence with that of other fish and animals, we hypothesize that the decreased binding of funiculosin could be due to a few amino acid replacements in the third and fourth transmembrane helix of the protein. In particular, the presence of methionine instead of alanine at position 125 seems to be largely responsible for the strong resistance to funiculosin and also to the partial resistance to myxothiazol in all fish mitochondria. Correlations between some residue substitutions in cytochrome b and the different effects of funiculosin in different species are also considered.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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