Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)
Identifieur interne : 000852 ( Istex/Curation ); précédent : 000851; suivant : 000853Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)
Auteurs : E. Ma An S [Espagne] ; J. Nú Ez ; S. Zanuy [Espagne] ; M. Carrillo [Espagne] ; F. Le Menn [France]Source :
- Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology [ 0305-0491 ] ; 1994.
Abstract
In this study, a competitive ELISA was developed for the quantification of vitellogenin (VTG) in sea bass. The purity of the antigen (VTG) and the specificity of the antibody (AbVTG) used in the assay, were assessed by ELISA and radial immunodiffusion, and no crossreactivity was observed with the male plasma. The sensitivity range of the ELISA was 1–60 ng/ml (85-20% of binding). The intra- and inter-assay variations (at 50% of binding) were 5.3% (N = 5) and 9.8% (N = 9), respectively. Annual VTG levels were quantified in animals reared under fish farming conditions and the VTG levels were correlated with the oocyte development.
Url:
DOI: 10.1016/0305-0491(94)90042-6
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000854
Links to Exploration step
ISTEX:F7DC129262A138DBDEB44D92E23EA3AC6D7357E3Curation
No country items
J. Nú Ez<affiliation><mods:affiliation>ORSTOM, Centre de Recherches Océanologiques (CRO), BP V18 Abidjan, Ivory Coast</mods:affiliation><wicri:noCountry code="subField">Coast</wicri:noCountry></affiliation>Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)</title><author><name sortKey="Ma An S, E" sort="Ma An S, E" uniqKey="Ma An S E" first="E." last="Ma An S">E. Ma An S</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea></affiliation></author><author><name sortKey="Nu Ez, J" sort="Nu Ez, J" uniqKey="Nu Ez J" first="J." last="Nú Ez">J. Nú Ez</name><affiliation><mods:affiliation>ORSTOM, Centre de Recherches Océanologiques (CRO), BP V18 Abidjan, Ivory Coast</mods:affiliation><wicri:noCountry code="subField">Coast</wicri:noCountry></affiliation></author><author><name sortKey="Zanuy, S" sort="Zanuy, S" uniqKey="Zanuy S" first="S." last="Zanuy">S. Zanuy</name><affiliation><mods:affiliation>Correspondence to: S. Zanuy, Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain, Fax: (964) 31-03-50.</mods:affiliation><wicri:noCountry code="subField">31-03-50.</wicri:noCountry></affiliation><affiliation wicri:level="1"><mods:affiliation>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea></affiliation></author><author><name sortKey="Carrillo, M" sort="Carrillo, M" uniqKey="Carrillo M" first="M." last="Carrillo">M. Carrillo</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea></affiliation></author><author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">F. Le Menn</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Biologie Marine, Université de Bordeaux I, Av. des Facultés, 33405 Talence Cedex, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Marine, Université de Bordeaux I, Av. des Facultés, 33405 Talence Cedex</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F7DC129262A138DBDEB44D92E23EA3AC6D7357E3</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1016/0305-0491(94)90042-6</idno><idno type="url">https://api.istex.fr/document/F7DC129262A138DBDEB44D92E23EA3AC6D7357E3/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000854</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000854</idno><idno type="wicri:Area/Istex/Curation">000852</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)</title><author><name sortKey="Ma An S, E" sort="Ma An S, E" uniqKey="Ma An S E" first="E." last="Ma An S">E. Ma An S</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea></affiliation></author><author><name sortKey="Nu Ez, J" sort="Nu Ez, J" uniqKey="Nu Ez J" first="J." last="Nú Ez">J. Nú Ez</name><affiliation><mods:affiliation>ORSTOM, Centre de Recherches Océanologiques (CRO), BP V18 Abidjan, Ivory Coast</mods:affiliation><wicri:noCountry code="subField">Coast</wicri:noCountry></affiliation></author><author><name sortKey="Zanuy, S" sort="Zanuy, S" uniqKey="Zanuy S" first="S." last="Zanuy">S. Zanuy</name><affiliation><mods:affiliation>Correspondence to: S. Zanuy, Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain, Fax: (964) 31-03-50.</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea></affiliation></author><author><name sortKey="Carrillo, M" sort="Carrillo, M" uniqKey="Carrillo M" first="M." last="Carrillo">M. Carrillo</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea></affiliation></author><author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">F. Le Menn</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Biologie Marine, Université de Bordeaux I, Av. des Facultés, 33405 Talence Cedex, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Marine, Université de Bordeaux I, Av. des Facultés, 33405 Talence Cedex</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title><title level="j" type="abbrev">CBBOLD</title><idno type="ISSN">0305-0491</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">107</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="217">217</biblScope><biblScope unit="page" to="223">223</biblScope></imprint><idno type="ISSN">0305-0491</idno></series><idno type="istex">F7DC129262A138DBDEB44D92E23EA3AC6D7357E3</idno><idno type="DOI">10.1016/0305-0491(94)90042-6</idno><idno type="PII">0305-0491(94)90042-6</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-0491</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, a competitive ELISA was developed for the quantification of vitellogenin (VTG) in sea bass. The purity of the antigen (VTG) and the specificity of the antibody (AbVTG) used in the assay, were assessed by ELISA and radial immunodiffusion, and no crossreactivity was observed with the male plasma. The sensitivity range of the ELISA was 1–60 ng/ml (85-20% of binding). The intra- and inter-assay variations (at 50% of binding) were 5.3% (N = 5) and 9.8% (N = 9), respectively. Annual VTG levels were quantified in animals reared under fish farming conditions and the VTG levels were correlated with the oocyte development.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000852 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000852 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:F7DC129262A138DBDEB44D92E23EA3AC6D7357E3
|texte= Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)
}}
| This area was generated with Dilib version V0.6.27. |  |