Metallothionein levels in sperm of various fish species
Identifieur interne : 000274 ( Istex/Curation ); précédent : 000273; suivant : 000275Metallothionein levels in sperm of various fish species
Auteurs : I. Fabrik [République tchèque] ; Z. Svobodova [République tchèque] ; V. Adam [République tchèque] ; S. Krizkova [République tchèque] ; L. Trnkova [République tchèque] ; M. Beklova [République tchèque] ; M. Rodina ; R. Kizek [République tchèque]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2008-08.
Abstract
In the present paper we determined low molecular heavy metal binding protein called metallothionein (MT) in fish spermatozoa sampled from four fish species (Acipenser baerii, Acipenser ruthenus, Perca fluviatilis and Sander lucioperca). The spermatozoa samples were analyzed with differential pulse voltammetry DPV (Brdicka reaction). The average content of MT in the fish sperm was 0.64 ± 0.55 μm. The highest content of MT was determined in spermatozoa of S. lucioperca (0.96 ± 0.86 μm), followed by P. fluviatilis (0.88 ± 0.11 μm), A. ruthenus (0.57 ± 0.60 μm) and A. baerii (0.43 ± 0.25 μm). The differences in the content of MT was probably related to the differences in age and perhaps to the health status based on the environmental conditions in which the tested specimens developed while also other still unknown factors may have been involved.
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DOI: 10.1111/j.1439-0426.2008.01132.x
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M. Rodina<affiliation><mods:affiliation>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia Ceske Budejovice, Vodnany</mods:affiliation>
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<front><div type="abstract" xml:lang="en">In the present paper we determined low molecular heavy metal binding protein called metallothionein (MT) in fish spermatozoa sampled from four fish species (Acipenser baerii, Acipenser ruthenus, Perca fluviatilis and Sander lucioperca). The spermatozoa samples were analyzed with differential pulse voltammetry DPV (Brdicka reaction). The average content of MT in the fish sperm was 0.64 ± 0.55 μm. The highest content of MT was determined in spermatozoa of S. lucioperca (0.96 ± 0.86 μm), followed by P. fluviatilis (0.88 ± 0.11 μm), A. ruthenus (0.57 ± 0.60 μm) and A. baerii (0.43 ± 0.25 μm). The differences in the content of MT was probably related to the differences in age and perhaps to the health status based on the environmental conditions in which the tested specimens developed while also other still unknown factors may have been involved.</div>
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