Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings
Identifieur interne : 001715 ( Istex/Corpus ); précédent : 001714; suivant : 001716Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings
Auteurs : M. Soltani ; M. Pourkazemi ; M. R. Ahmadi ; A. Taherimirghead ; D. L. Merrifield ; A. S. MasoulehSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2013-06.
Abstract
Lactic acid bacteria (LAB) are often found as sub‐dominant microbial components in the gastrointestinal (GI) tract of fish and have positive effects on the host. They are generally promising candidates for probiotics and can act as substances to improving both native immune responses and growth performance of animals including fish. In the present study the Persian sturgeon Acipenser persicus autochthonous intestinal LAB were investigated. A total of 90 sturgeon fingerlings were sampled and LAB were isolated and enumerated on MRS agar. Initial identification of 47 LAB isolates using standard biochemical and phenotypic tests revealed five dominant phenotypes. Twenty‐one isolates, representatives from each of these phenotypes, were subsequently identified by 16S rRNA sequence analysis. The results showed that cultivable authochthonous LAB ranged from log 2.93 to 5.61 CFU g−1 intestine, with a mean of 4.38 ± 0.58 CFU g−1. 16S rRNA sequence analysis revealed that the LAB community was dominated by Lactococcus spp., with Lactococcus garvieae and Lactococcus lactis accounting for 42.55 and 36.17% of the LAB population, respectively. Pediococcus pentosaceus (14.90%), Weissella cibaria (4.25%) and Enterococcus faecalis (2.13%) were identified as minor components of the LAB community. This is the first report of these LAB as members of the microbial community in the intestine of Acipenser persicus. The results show that both potentially pathogenic (i.e. Lactococcus garvieae) and probiotic (i.e. Lactococcus lactis and Weissella cibaria) LAB inhabit the Persian sturgeon GI tract. Future studies should seek to elucidate the relevance of these species to the host.
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DOI: 10.1111/jai.12107
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Soltani</name><affiliation><mods:affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</mods:affiliation></affiliation><affiliation><mods:affiliation>Center of Excellence of Aquatic Animal Health, University of Tehran, Tehran, Iran</mods:affiliation></affiliation><affiliation><mods:affiliation>: Dr Mehdi Soltani, Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, PO Box 14155‐6453, Tehran, Iran.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: msoltani@ut.ac.ir</mods:affiliation></affiliation></author><author><name sortKey="Pourkazemi, M" sort="Pourkazemi, M" uniqKey="Pourkazemi M" first="M." last="Pourkazemi">M. Pourkazemi</name><affiliation><mods:affiliation>International Sturgeon Research Institute, Rasht, Iran</mods:affiliation></affiliation></author><author><name sortKey="Ahmadi, M R" sort="Ahmadi, M R" uniqKey="Ahmadi M" first="M. R." last="Ahmadi">M. R. Ahmadi</name><affiliation><mods:affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</mods:affiliation></affiliation></author><author><name sortKey="Taherimirghead, A" sort="Taherimirghead, A" uniqKey="Taherimirghead A" first="A." last="Taherimirghead">A. Taherimirghead</name><affiliation><mods:affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</mods:affiliation></affiliation></author><author><name sortKey="Merrifield, D L" sort="Merrifield, D L" uniqKey="Merrifield D" first="D. L." last="Merrifield">D. L. Merrifield</name><affiliation><mods:affiliation>Aquaculture and Fish Nutrition Research Group, School of Biological Science, Plymouth University, Devon, Plymouth, UK</mods:affiliation></affiliation></author><author><name sortKey="Masouleh, A S" sort="Masouleh, A S" uniqKey="Masouleh A" first="A. S." last="Masouleh">A. S. Masouleh</name><affiliation><mods:affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</mods:affiliation></affiliation><affiliation><mods:affiliation>International Sturgeon Research Institute, Rasht, Iran</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-06">2013-06</date><biblScope unit="volume">29</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="494">494</biblScope><biblScope unit="page" to="498">498</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">35DDB92C480198F4857BBEF22155C01EE4C88122</idno><idno type="DOI">10.1111/jai.12107</idno><idno type="ArticleID">JAI12107</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Lactic acid bacteria (LAB) are often found as sub‐dominant microbial components in the gastrointestinal (GI) tract of fish and have positive effects on the host. They are generally promising candidates for probiotics and can act as substances to improving both native immune responses and growth performance of animals including fish. In the present study the Persian sturgeon Acipenser persicus autochthonous intestinal LAB were investigated. A total of 90 sturgeon fingerlings were sampled and LAB were isolated and enumerated on MRS agar. Initial identification of 47 LAB isolates using standard biochemical and phenotypic tests revealed five dominant phenotypes. Twenty‐one isolates, representatives from each of these phenotypes, were subsequently identified by 16S rRNA sequence analysis. The results showed that cultivable authochthonous LAB ranged from log 2.93 to 5.61 CFU g−1 intestine, with a mean of 4.38 ± 0.58 CFU g−1. 16S rRNA sequence analysis revealed that the LAB community was dominated by Lactococcus spp., with Lactococcus garvieae and Lactococcus lactis accounting for 42.55 and 36.17% of the LAB population, respectively. Pediococcus pentosaceus (14.90%), Weissella cibaria (4.25%) and Enterococcus faecalis (2.13%) were identified as minor components of the LAB community. This is the first report of these LAB as members of the microbial community in the intestine of Acipenser persicus. The results show that both potentially pathogenic (i.e. Lactococcus garvieae) and probiotic (i.e. Lactococcus lactis and Weissella cibaria) LAB inhabit the Persian sturgeon GI tract. Future studies should seek to elucidate the relevance of these species to the host.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>M. Soltani</name><affiliations><json:string>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</json:string><json:string>Center of Excellence of Aquatic Animal Health, University of Tehran, Tehran, Iran</json:string><json:string>: Dr Mehdi Soltani, Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, PO Box 14155‐6453, Tehran, Iran.E‐mail:</json:string><json:string>E-mail: msoltani@ut.ac.ir</json:string></affiliations></json:item><json:item><name>M. Pourkazemi</name><affiliations><json:string>International Sturgeon Research Institute, Rasht, Iran</json:string></affiliations></json:item><json:item><name>M.R. Ahmadi</name><affiliations><json:string>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</json:string></affiliations></json:item><json:item><name>A. 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A total of 90 sturgeon fingerlings were sampled and LAB were isolated and enumerated on MRS agar. Initial identification of 47 LAB isolates using standard biochemical and phenotypic tests revealed five dominant phenotypes. Twenty‐one isolates, representatives from each of these phenotypes, were subsequently identified by 16S rRNA sequence analysis. The results showed that cultivable authochthonous LAB ranged from log 2.93 to 5.61 CFU g−1 intestine, with a mean of 4.38 ± 0.58 CFU g−1. 16S rRNA sequence analysis revealed that the LAB community was dominated by Lactococcus spp., with Lactococcus garvieae and Lactococcus lactis accounting for 42.55 and 36.17% of the LAB population, respectively. Pediococcus pentosaceus (14.90%), Weissella cibaria (4.25%) and Enterococcus faecalis (2.13%) were identified as minor components of the LAB community. This is the first report of these LAB as members of the microbial community in the intestine of Acipenser persicus. The results show that both potentially pathogenic (i.e. Lactococcus garvieae) and probiotic (i.e. Lactococcus lactis and Weissella cibaria) LAB inhabit the Persian sturgeon GI tract. 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L.</forename><surname>Merrifield</surname></persName><affiliation>Aquaculture and Fish Nutrition Research Group, School of Biological Science, Plymouth University, Devon, Plymouth, UK</affiliation></author><author xml:id="author-6"><persName><forename type="first">A. S.</forename><surname>Masouleh</surname></persName><affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</affiliation><affiliation>International Sturgeon Research Institute, Rasht, Iran</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-06"></date><biblScope unit="volume">29</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="494">494</biblScope><biblScope unit="page" to="498">498</biblScope></imprint></monogr><idno type="istex">35DDB92C480198F4857BBEF22155C01EE4C88122</idno><idno type="DOI">10.1111/jai.12107</idno><idno type="ArticleID">JAI12107</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-10-19</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Lactic acid bacteria (LAB) are often found as sub‐dominant microbial components in the gastrointestinal (GI) tract of fish and have positive effects on the host. They are generally promising candidates for probiotics and can act as substances to improving both native immune responses and growth performance of animals including fish. In the present study the Persian sturgeon Acipenser persicus autochthonous intestinal LAB were investigated. A total of 90 sturgeon fingerlings were sampled and LAB were isolated and enumerated on MRS agar. Initial identification of 47 LAB isolates using standard biochemical and phenotypic tests revealed five dominant phenotypes. Twenty‐one isolates, representatives from each of these phenotypes, were subsequently identified by 16S rRNA sequence analysis. The results showed that cultivable authochthonous LAB ranged from log 2.93 to 5.61 CFU g−1 intestine, with a mean of 4.38 ± 0.58 CFU g−1. 16S rRNA sequence analysis revealed that the LAB community was dominated by Lactococcus spp., with Lactococcus garvieae and Lactococcus lactis accounting for 42.55 and 36.17% of the LAB population, respectively. Pediococcus pentosaceus (14.90%), Weissella cibaria (4.25%) and Enterococcus faecalis (2.13%) were identified as minor components of the LAB community. This is the first report of these LAB as members of the microbial community in the intestine of Acipenser persicus. The results show that both potentially pathogenic (i.e. Lactococcus garvieae) and probiotic (i.e. Lactococcus lactis and Weissella cibaria) LAB inhabit the Persian sturgeon GI tract. Future studies should seek to elucidate the relevance of these species to the host.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Original Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-05-07">Received</change><change when="2012-08-13">Registration</change><change when="2012-10-19">Created</change><change when="2013-06">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/35DDB92C480198F4857BBEF22155C01EE4C88122/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:id="jai12107" xml:lang="en"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id></idGroup><titleGroup><title sort="JOURNAL OF APPLIED ICHTHYOLOGY" type="main">Journal of Applied Ichthyology</title><title type="short">J. 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Soltani et al.</title></titleGroup><creators><creator affiliationRef="#jai12107-aff-0001 #jai12107-aff-0002" corresponding="yes" creatorRole="author" xml:id="jai12107-cr-0001"><personName><givenNames>M.</givenNames> <familyName>Soltani</familyName></personName></creator><creator affiliationRef="#jai12107-aff-0003" creatorRole="author" xml:id="jai12107-cr-0002"><personName><givenNames>M.</givenNames> <familyName>Pourkazemi</familyName></personName></creator><creator affiliationRef="#jai12107-aff-0001" creatorRole="author" xml:id="jai12107-cr-0003"><personName><givenNames>M.R.</givenNames> <familyName>Ahmadi</familyName></personName></creator><creator affiliationRef="#jai12107-aff-0001" creatorRole="author" xml:id="jai12107-cr-0004"><personName><givenNames>A.</givenNames> <familyName>Taherimirghead</familyName></personName></creator><creator affiliationRef="#jai12107-aff-0004" creatorRole="author" xml:id="jai12107-cr-0005"><personName><givenNames>D. L.</givenNames> <familyName>Merrifield</familyName></personName></creator><creator affiliationRef="#jai12107-aff-0001 #jai12107-aff-0003" creatorRole="author" xml:id="jai12107-cr-0006"><personName><givenNames>A. S.</givenNames> <familyName>Masouleh</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="IR" type="organization" xml:id="jai12107-aff-0001"><orgDiv>Department of Aquatic Animal Health</orgDiv> <orgDiv>Faculty of Veterinary Medicine</orgDiv> <orgName>University of Tehran</orgName> <address><city>Tehran</city> <country>Iran</country></address></affiliation><affiliation countryCode="IR" type="organization" xml:id="jai12107-aff-0002"><orgDiv>Center of Excellence of Aquatic Animal Health</orgDiv> <orgName>University of Tehran</orgName> <address><city>Tehran</city> <country>Iran</country></address></affiliation><affiliation countryCode="IR" type="organization" xml:id="jai12107-aff-0003"><orgName>International Sturgeon Research Institute</orgName> <address><city>Rasht</city> <country>Iran</country></address></affiliation><affiliation countryCode="GB" type="organization" xml:id="jai12107-aff-0004"><orgDiv>Aquaculture and Fish Nutrition Research Group</orgDiv> <orgDiv>School of Biological Science</orgDiv> <orgName>Plymouth University</orgName> <address><city>Plymouth</city> <countryPart>Devon</countryPart> <country>UK</country></address></affiliation></affiliationGroup><fundingInfo><fundingAgency>Research Council of the University of Tehran</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:id="jai12107-abs-0001"><title type="main">Summary</title><p>Lactic acid bacteria (<fc>LAB</fc>) are often found as sub‐dominant microbial components in the gastrointestinal (<fc>GI</fc>) tract of fish and have positive effects on the host. They are generally promising candidates for probiotics and can act as substances to improving both native immune responses and growth performance of animals including fish. In the present study the Persian sturgeon <i>Acipenser persicus</i> autochthonous intestinal <fc>LAB</fc> were investigated. A total of 90 sturgeon fingerlings were sampled and <fc>LAB</fc> were isolated and enumerated on <fc>MRS</fc> agar. Initial identification of 47 <fc>LAB</fc> isolates using standard biochemical and phenotypic tests revealed five dominant phenotypes. Twenty‐one isolates, representatives from each of these phenotypes, were subsequently identified by 16S <fc>rRNA</fc> sequence analysis. The results showed that cultivable authochthonous <fc>LAB</fc> ranged from log 2.93 to 5.61 CFU g<sup>−1</sup> intestine, with a mean of 4.38 ± 0.58 CFU g<sup>−1</sup>. 16S <fc>rRNA</fc> sequence analysis revealed that the <fc>LAB</fc> community was dominated by <i><fc>L</fc>actococcus</i> spp., with <i><fc>L</fc>actococcus garvieae</i> and <i><fc>L</fc>actococcus lactis</i> accounting for 42.55 and 36.17% of the <fc>LAB</fc> population, respectively. <i><fc>P</fc>ediococcus pentosaceus</i> (14.90%), <i><fc>W</fc>eissella cibaria</i> (4.25%) and <i><fc>E</fc>nterococcus faecalis</i> (2.13%) were identified as minor components of the <fc>LAB</fc> community. This is the first report of these <fc>LAB</fc> as members of the microbial community in the intestine of <i><fc>A</fc>cipenser persicus</i>. The results show that both potentially pathogenic (i.e. <i><fc>L</fc>actococcus garvieae</i>) and probiotic (i.e. <i><fc>L</fc>actococcus lactis</i> and <i><fc>W</fc>eissella cibaria</i>) <fc>LAB</fc> inhabit the <fc>P</fc>ersian sturgeon <fc>GI</fc> tract. Future studies should seek to elucidate the relevance of these species to the host.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Soltani</namePart><affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</affiliation><affiliation>Center of Excellence of Aquatic Animal Health, University of Tehran, Tehran, Iran</affiliation><affiliation>: Dr Mehdi Soltani, Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, PO Box 14155‐6453, Tehran, Iran.E‐mail:</affiliation><affiliation>E-mail: msoltani@ut.ac.ir</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Pourkazemi</namePart><affiliation>International Sturgeon Research Institute, Rasht, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.R.</namePart><namePart type="family">Ahmadi</namePart><affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Taherimirghead</namePart><affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D. L.</namePart><namePart type="family">Merrifield</namePart><affiliation>Aquaculture and Fish Nutrition Research Group, School of Biological Science, Plymouth University, Devon, Plymouth, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. S.</namePart><namePart type="family">Masouleh</namePart><affiliation>Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran</affiliation><affiliation>International Sturgeon Research Institute, Rasht, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2013-06</dateIssued><dateCreated encoding="w3cdtf">2012-10-19</dateCreated><dateCaptured encoding="w3cdtf">2012-05-07</dateCaptured><dateValid encoding="w3cdtf">2012-08-13</dateValid><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Lactic acid bacteria (LAB) are often found as sub‐dominant microbial components in the gastrointestinal (GI) tract of fish and have positive effects on the host. They are generally promising candidates for probiotics and can act as substances to improving both native immune responses and growth performance of animals including fish. In the present study the Persian sturgeon Acipenser persicus autochthonous intestinal LAB were investigated. A total of 90 sturgeon fingerlings were sampled and LAB were isolated and enumerated on MRS agar. Initial identification of 47 LAB isolates using standard biochemical and phenotypic tests revealed five dominant phenotypes. Twenty‐one isolates, representatives from each of these phenotypes, were subsequently identified by 16S rRNA sequence analysis. The results showed that cultivable authochthonous LAB ranged from log 2.93 to 5.61 CFU g−1 intestine, with a mean of 4.38 ± 0.58 CFU g−1. 16S rRNA sequence analysis revealed that the LAB community was dominated by Lactococcus spp., with Lactococcus garvieae and Lactococcus lactis accounting for 42.55 and 36.17% of the LAB population, respectively. Pediococcus pentosaceus (14.90%), Weissella cibaria (4.25%) and Enterococcus faecalis (2.13%) were identified as minor components of the LAB community. This is the first report of these LAB as members of the microbial community in the intestine of Acipenser persicus. The results show that both potentially pathogenic (i.e. Lactococcus garvieae) and probiotic (i.e. Lactococcus lactis and Weissella cibaria) LAB inhabit the Persian sturgeon GI tract. Future studies should seek to elucidate the relevance of these species to the host.</abstract><note type="funding">Research Council of the University of Tehran</note><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><titleInfo type="abbreviated"><title>J. Appl. Ichthyol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Original Article</topic></subject><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2013</date><detail type="volume"><caption>vol.</caption><number>29</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>494</start><end>498</end><total>5</total></extent></part></relatedItem><identifier type="istex">35DDB92C480198F4857BBEF22155C01EE4C88122</identifier><identifier type="DOI">10.1111/jai.12107</identifier><identifier type="ArticleID">JAI12107</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2013 Blackwell Verlag GmbH© 2012 Blackwell Verlag GmbH</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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