Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures
Identifieur interne : 001694 ( Istex/Corpus ); précédent : 001693; suivant : 001695Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures
Auteurs : U. Nebel ; Ch. Mülling ; K. BudrasSource :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
The aim of this study was to characterize the ultrastructure of three‐dimensional keratinocyte colonies in relation to ex vivo bovine hoof epidermis. Keratinocytes were isolated from the claw and cultured in equal parts of Dulbecco's modified Eagle's medium (Sigma, Taufkirchen) supplemented with 10% fetal bovine serum (Sigma, Taufkirchen) and serum‐free MCDB 153 complete medium (Biochrom, Berlin). Cells were allowed to grow until formation of colonies occurred. Then the colonies were isolated by removing the surrounding cells with a cell scraper every 3 days. The colonies were harvested when the reached diameters of 6–8 mm and were fixed in Karnovsky's solution. They were routinely processed for the embedding in Agar 100. Semithin and ultrathin sections were prepared and examined under the light and transmission electron microscope. These colonies revealed the morphological properties and tissue architecture, which are specific for claw epidermis in vivo. We detected up to 22 layers of keratinocytes and could discover between three stages of differentiation: a basal cell layer, followed by several cell layers corresponding morphologically to the cells of the stratum spinosum and a few cornified superficial cell layers. Throughout all layers cells were connected by desmosomes, which were linked to the cytoskeleton by dense bundles of filaments. In addition we observed the formation of desmosomes. The cells of the upper un‐cornified layers generated an cornified envelope. In the following layers cell death and subsequent cornification accompanied by the characteristic alterations of the nuclei occurred. The cells at the superficial layers were filled by electron dense, solid horn masses. Additionally we detected intercellular cementing substance in the intercellular space between the upper layers. Our electron microscopical characterization of the colonies provides evidence that the bovine hoof keratinocytes invitro undergo basically a similar pattern of proliferation and differentiation like in the claw in vivo. These findings provided a basis for further studies like the long‐term culture and differentiation of bovine hoof keratinocytes in perfusion chambers.
Url:
DOI: 10.1111/j.1439-0264.2005.00669_84.x
Links to Exploration step
ISTEX:7E67D120C19CE7BDB27F4E843A7032449884A795Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title><author><name sortKey="Nebel, U" sort="Nebel, U" uniqKey="Nebel U" first="U." last="Nebel">U. Nebel</name><affiliation><mods:affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Mulling, Ch" sort="Mulling, Ch" uniqKey="Mulling C" first="Ch." last="Mülling">Ch. Mülling</name><affiliation><mods:affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Budras, K" sort="Budras, K" uniqKey="Budras K" first="K." last="Budras">K. Budras</name><affiliation><mods:affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7E67D120C19CE7BDB27F4E843A7032449884A795</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1111/j.1439-0264.2005.00669_84.x</idno><idno type="url">https://api.istex.fr/document/7E67D120C19CE7BDB27F4E843A7032449884A795/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001694</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001694</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title><author><name sortKey="Nebel, U" sort="Nebel, U" uniqKey="Nebel U" first="U." last="Nebel">U. Nebel</name><affiliation><mods:affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Mulling, Ch" sort="Mulling, Ch" uniqKey="Mulling C" first="Ch." last="Mülling">Ch. Mülling</name><affiliation><mods:affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Budras, K" sort="Budras, K" uniqKey="Budras K" first="K." last="Budras">K. Budras</name><affiliation><mods:affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Anatomia, Histologia, Embryologia</title><idno type="ISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12">2005-12</date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="37">37</biblScope><biblScope unit="page" to="37">37</biblScope></imprint><idno type="ISSN">0340-2096</idno></series><idno type="istex">7E67D120C19CE7BDB27F4E843A7032449884A795</idno><idno type="DOI">10.1111/j.1439-0264.2005.00669_84.x</idno><idno type="ArticleID">AHE669_84_84</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0340-2096</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to characterize the ultrastructure of three‐dimensional keratinocyte colonies in relation to ex vivo bovine hoof epidermis. Keratinocytes were isolated from the claw and cultured in equal parts of Dulbecco's modified Eagle's medium (Sigma, Taufkirchen) supplemented with 10% fetal bovine serum (Sigma, Taufkirchen) and serum‐free MCDB 153 complete medium (Biochrom, Berlin). Cells were allowed to grow until formation of colonies occurred. Then the colonies were isolated by removing the surrounding cells with a cell scraper every 3 days. The colonies were harvested when the reached diameters of 6–8 mm and were fixed in Karnovsky's solution. They were routinely processed for the embedding in Agar 100. Semithin and ultrathin sections were prepared and examined under the light and transmission electron microscope. These colonies revealed the morphological properties and tissue architecture, which are specific for claw epidermis in vivo. We detected up to 22 layers of keratinocytes and could discover between three stages of differentiation: a basal cell layer, followed by several cell layers corresponding morphologically to the cells of the stratum spinosum and a few cornified superficial cell layers. Throughout all layers cells were connected by desmosomes, which were linked to the cytoskeleton by dense bundles of filaments. In addition we observed the formation of desmosomes. The cells of the upper un‐cornified layers generated an cornified envelope. In the following layers cell death and subsequent cornification accompanied by the characteristic alterations of the nuclei occurred. The cells at the superficial layers were filled by electron dense, solid horn masses. Additionally we detected intercellular cementing substance in the intercellular space between the upper layers. Our electron microscopical characterization of the colonies provides evidence that the bovine hoof keratinocytes invitro undergo basically a similar pattern of proliferation and differentiation like in the claw in vivo. These findings provided a basis for further studies like the long‐term culture and differentiation of bovine hoof keratinocytes in perfusion chambers.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>U. Nebel</name><affiliations><json:string>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</json:string></affiliations></json:item><json:item><name>Ch. Mülling</name><affiliations><json:string>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</json:string></affiliations></json:item><json:item><name>K. ‐D. Budras</name><affiliations><json:string>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</json:string></affiliations></json:item></author><articleId><json:string>AHE669_84_84</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>abstract</json:string></originalGenre><abstract>The aim of this study was to characterize the ultrastructure of three‐dimensional keratinocyte colonies in relation to ex vivo bovine hoof epidermis. Keratinocytes were isolated from the claw and cultured in equal parts of Dulbecco's modified Eagle's medium (Sigma, Taufkirchen) supplemented with 10% fetal bovine serum (Sigma, Taufkirchen) and serum‐free MCDB 153 complete medium (Biochrom, Berlin). Cells were allowed to grow until formation of colonies occurred. Then the colonies were isolated by removing the surrounding cells with a cell scraper every 3 days. The colonies were harvested when the reached diameters of 6–8 mm and were fixed in Karnovsky's solution. They were routinely processed for the embedding in Agar 100. Semithin and ultrathin sections were prepared and examined under the light and transmission electron microscope. These colonies revealed the morphological properties and tissue architecture, which are specific for claw epidermis in vivo. We detected up to 22 layers of keratinocytes and could discover between three stages of differentiation: a basal cell layer, followed by several cell layers corresponding morphologically to the cells of the stratum spinosum and a few cornified superficial cell layers. Throughout all layers cells were connected by desmosomes, which were linked to the cytoskeleton by dense bundles of filaments. In addition we observed the formation of desmosomes. The cells of the upper un‐cornified layers generated an cornified envelope. In the following layers cell death and subsequent cornification accompanied by the characteristic alterations of the nuclei occurred. The cells at the superficial layers were filled by electron dense, solid horn masses. Additionally we detected intercellular cementing substance in the intercellular space between the upper layers. Our electron microscopical characterization of the colonies provides evidence that the bovine hoof keratinocytes invitro undergo basically a similar pattern of proliferation and differentiation like in the claw in vivo. These findings provided a basis for further studies like the long‐term culture and differentiation of bovine hoof keratinocytes in perfusion chambers.</abstract><qualityIndicators><score>8.5</score><pdfVersion>1.4</pdfVersion><pdfPageSize>595 x 842 pts (A4)</pdfPageSize><refBibsNative>false</refBibsNative><abstractCharCount>2197</abstractCharCount><pdfWordCount>39539</pdfWordCount><pdfCharCount>256516</pdfCharCount><pdfPageCount>59</pdfPageCount><abstractWordCount>316</abstractWordCount></qualityIndicators><title>Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title><genre><json:string>abstract</json:string></genre><host><volume>34</volume><publisherId><json:string>AHE</json:string></publisherId><pages><last>37</last><first>37</first></pages><issn><json:string>0340-2096</json:string></issn><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1439-0264</json:string></eissn><title>Anatomia, Histologia, Embryologia</title><doi><json:string>10.1111/(ISSN)1439-0264</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>veterinary sciences</json:string><json:string>anatomy & morphology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>developmental biology</json:string></scienceMetrix></categories><publicationDate>2005</publicationDate><copyrightDate>2005</copyrightDate><doi><json:string>10.1111/j.1439-0264.2005.00669_84.x</json:string></doi><id>7E67D120C19CE7BDB27F4E843A7032449884A795</id><score>0.024610277</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/7E67D120C19CE7BDB27F4E843A7032449884A795/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/7E67D120C19CE7BDB27F4E843A7032449884A795/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/7E67D120C19CE7BDB27F4E843A7032449884A795/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><availability><p>WILEY</p></availability><date>2005</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title><author xml:id="author-1"><persName><forename type="first">U.</forename><surname>Nebel</surname></persName><affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">Ch.</forename><surname>Mülling</surname></persName><affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</affiliation></author><author xml:id="author-3"><persName><forename type="first">K. ‐D.</forename><surname>Budras</surname></persName><affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</affiliation></author></analytic><monogr><title level="j">Anatomia, Histologia, Embryologia</title><idno type="pISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><idno type="DOI">10.1111/(ISSN)1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12"></date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="37">37</biblScope><biblScope unit="page" to="37">37</biblScope></imprint></monogr><idno type="istex">7E67D120C19CE7BDB27F4E843A7032449884A795</idno><idno type="DOI">10.1111/j.1439-0264.2005.00669_84.x</idno><idno type="ArticleID">AHE669_84_84</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The aim of this study was to characterize the ultrastructure of three‐dimensional keratinocyte colonies in relation to ex vivo bovine hoof epidermis. Keratinocytes were isolated from the claw and cultured in equal parts of Dulbecco's modified Eagle's medium (Sigma, Taufkirchen) supplemented with 10% fetal bovine serum (Sigma, Taufkirchen) and serum‐free MCDB 153 complete medium (Biochrom, Berlin). Cells were allowed to grow until formation of colonies occurred. Then the colonies were isolated by removing the surrounding cells with a cell scraper every 3 days. The colonies were harvested when the reached diameters of 6–8 mm and were fixed in Karnovsky's solution. They were routinely processed for the embedding in Agar 100. Semithin and ultrathin sections were prepared and examined under the light and transmission electron microscope. These colonies revealed the morphological properties and tissue architecture, which are specific for claw epidermis in vivo. We detected up to 22 layers of keratinocytes and could discover between three stages of differentiation: a basal cell layer, followed by several cell layers corresponding morphologically to the cells of the stratum spinosum and a few cornified superficial cell layers. Throughout all layers cells were connected by desmosomes, which were linked to the cytoskeleton by dense bundles of filaments. In addition we observed the formation of desmosomes. The cells of the upper un‐cornified layers generated an cornified envelope. In the following layers cell death and subsequent cornification accompanied by the characteristic alterations of the nuclei occurred. The cells at the superficial layers were filled by electron dense, solid horn masses. Additionally we detected intercellular cementing substance in the intercellular space between the upper layers. Our electron microscopical characterization of the colonies provides evidence that the bovine hoof keratinocytes invitro undergo basically a similar pattern of proliferation and differentiation like in the claw in vivo. These findings provided a basis for further studies like the long‐term culture and differentiation of bovine hoof keratinocytes in perfusion chambers.</p></abstract></profileDesc><revisionDesc><change when="2005-12">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-02-9">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/7E67D120C19CE7BDB27F4E843A7032449884A795/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley component found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Verlag GmbH</publisherName><publisherLoc>Berlin, Germany</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0264</doi><issn type="print">0340-2096</issn><issn type="electronic">1439-0264</issn><idGroup><id type="product" value="AHE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ANATOMIA HISTOLOGIA EMBRYOLOGIA">Anatomia, Histologia, Embryologia</title></titleGroup></publicationMeta><publicationMeta level="part" position="12000"><doi origin="wiley">10.1111/ahe.2005.34.issue-s1</doi><numberingGroup><numbering type="journalVolume" number="34">34</numbering><numbering type="supplement">s1</numbering></numberingGroup><coverDate startDate="2005-12">December 2005</coverDate></publicationMeta><publicationMeta level="unit" type="abstract" position="1" status="forIssue"><doi origin="wiley">10.1111/j.1439-0264.2005.00669_84.x</doi><idGroup><id type="unit" value="AHE669_84_84"></id></idGroup><titleGroup><title type="tocHeading1">Abstracts of the XXVth Congress of the European Association of Veterinary Anatomists (EAVA), Oslo, 28–31 July 2004</title></titleGroup><eventGroup><event type="firstOnline" date="2005-11-22"></event><event type="publishedOnlineFinalForm" date="2005-11-22"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:Header result:Header" date="2010-04-14"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="37">37</numbering><numbering type="pageLast" number="37">37</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:AHE.AHE669_84_84.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title><title type="short">Abstracts</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1"><personName><givenNames>U.</givenNames><familyName>Nebel</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>Ch.</givenNames><familyName>Mülling</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>K. ‐D.</givenNames><familyName>Budras</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="DE"><unparsedAffiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The aim of this study was to characterize the ultrastructure of three‐dimensional keratinocyte colonies in relation to ex vivo bovine hoof epidermis. Keratinocytes were isolated from the claw and cultured in equal parts of Dulbecco's modified Eagle's medium (Sigma, Taufkirchen) supplemented with 10% fetal bovine serum (Sigma, Taufkirchen) and serum‐free MCDB 153 complete medium (Biochrom, Berlin). Cells were allowed to grow until formation of colonies occurred. Then the colonies were isolated by removing the surrounding cells with a cell scraper every 3 days. The colonies were harvested when the reached diameters of 6–8 mm and were fixed in Karnovsky's solution. They were routinely processed for the embedding in Agar 100. Semithin and ultrathin sections were prepared and examined under the light and transmission electron microscope.</p><p>These colonies revealed the morphological properties and tissue architecture, which are specific for claw epidermis <i>in vivo</i>. We detected up to 22 layers of keratinocytes and could discover between three stages of differentiation: a basal cell layer, followed by several cell layers corresponding morphologically to the cells of the stratum spinosum and a few cornified superficial cell layers. Throughout all layers cells were connected by desmosomes, which were linked to the cytoskeleton by dense bundles of filaments. In addition we observed the formation of desmosomes. The cells of the upper un‐cornified layers generated an cornified envelope. In the following layers cell death and subsequent cornification accompanied by the characteristic alterations of the nuclei occurred. The cells at the superficial layers were filled by electron dense, solid horn masses. Additionally we detected intercellular cementing substance in the intercellular space between the upper layers. Our electron microscopical characterization of the colonies provides evidence that the bovine hoof keratinocytes <i>in</i><i>vitro</i> undergo basically a similar pattern of proliferation and differentiation like in the claw <i>in vivo</i>. These findings provided a basis for further studies like the long‐term culture and differentiation of bovine hoof keratinocytes in perfusion chambers.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Abstracts</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures</title></titleInfo><name type="personal"><namePart type="given">U.</namePart><namePart type="family">Nebel</namePart><affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ch.</namePart><namePart type="family">Mülling</namePart><affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. ‐D.</namePart><namePart type="family">Budras</namePart><affiliation>Department of Veterinary Anatomy, Freie Universität Berlin, Koserstr. 20, D14195 Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Verlag GmbH</publisher><place><placeTerm type="text">Berlin, Germany</placeTerm></place><dateIssued encoding="w3cdtf">2005-12</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The aim of this study was to characterize the ultrastructure of three‐dimensional keratinocyte colonies in relation to ex vivo bovine hoof epidermis. Keratinocytes were isolated from the claw and cultured in equal parts of Dulbecco's modified Eagle's medium (Sigma, Taufkirchen) supplemented with 10% fetal bovine serum (Sigma, Taufkirchen) and serum‐free MCDB 153 complete medium (Biochrom, Berlin). Cells were allowed to grow until formation of colonies occurred. Then the colonies were isolated by removing the surrounding cells with a cell scraper every 3 days. The colonies were harvested when the reached diameters of 6–8 mm and were fixed in Karnovsky's solution. They were routinely processed for the embedding in Agar 100. Semithin and ultrathin sections were prepared and examined under the light and transmission electron microscope. These colonies revealed the morphological properties and tissue architecture, which are specific for claw epidermis in vivo. We detected up to 22 layers of keratinocytes and could discover between three stages of differentiation: a basal cell layer, followed by several cell layers corresponding morphologically to the cells of the stratum spinosum and a few cornified superficial cell layers. Throughout all layers cells were connected by desmosomes, which were linked to the cytoskeleton by dense bundles of filaments. In addition we observed the formation of desmosomes. The cells of the upper un‐cornified layers generated an cornified envelope. In the following layers cell death and subsequent cornification accompanied by the characteristic alterations of the nuclei occurred. The cells at the superficial layers were filled by electron dense, solid horn masses. Additionally we detected intercellular cementing substance in the intercellular space between the upper layers. Our electron microscopical characterization of the colonies provides evidence that the bovine hoof keratinocytes invitro undergo basically a similar pattern of proliferation and differentiation like in the claw in vivo. These findings provided a basis for further studies like the long‐term culture and differentiation of bovine hoof keratinocytes in perfusion chambers.</abstract><relatedItem type="host"><titleInfo><title>Anatomia, Histologia, Embryologia</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0340-2096</identifier><identifier type="eISSN">1439-0264</identifier><identifier type="DOI">10.1111/(ISSN)1439-0264</identifier><identifier type="PublisherID">AHE</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>37</start><end>37</end></extent></part></relatedItem><identifier type="istex">7E67D120C19CE7BDB27F4E843A7032449884A795</identifier><identifier type="DOI">10.1111/j.1439-0264.2005.00669_84.x</identifier><identifier type="ArticleID">AHE669_84_84</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Verlag GmbH</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001694 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001694 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:7E67D120C19CE7BDB27F4E843A7032449884A795
|texte= Ultrastructure of Three‐dimensional Bovine Hoof Keratinocyte Cultures
}}
| This area was generated with Dilib version V0.6.27. |  |