Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results
Identifieur interne : 001677 ( Istex/Corpus ); précédent : 001676; suivant : 001678Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results
Auteurs : A. Alexandre- Pires ; G. Ferreira-Dias ; L. Mateus ; L. Mendes- Jorge ; J. M. Cifuentes ; N. AlemaSource :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti‐angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected post mortem from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis (‘Scion Image, NIH, USA’) based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one‐way ANOVA and post‐comparison tests. There was a significant increase in microvascular areas in November (P < 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February (P < 0.05) and November (P < 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non‐breeding season in the male cat.
Url:
DOI: 10.1111/j.1439-0264.2005.00669_4.x
Links to Exploration step
ISTEX:356E1F0514CB08A534C75719FD6D48864D6DABDALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title><author><name sortKey="Alexandre Pires, A" sort="Alexandre Pires, A" uniqKey="Alexandre Pires A" first="A." last="Alexandre- Pires">A. Alexandre- Pires</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Ferreira Ias, G" sort="Ferreira Ias, G" uniqKey="Ferreira Ias G" first="G." last="Ferreira-Dias">G. Ferreira-Dias</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Mateus, L" sort="Mateus, L" uniqKey="Mateus L" first="L." last="Mateus">L. Mateus</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Mendes Jorge, L" sort="Mendes Jorge, L" uniqKey="Mendes Jorge L" first="L." last="Mendes- Jorge">L. Mendes- Jorge</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Cifuentes, J M" sort="Cifuentes, J M" uniqKey="Cifuentes J" first="J. M." last="Cifuentes">J. M. Cifuentes</name><affiliation><mods:affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</mods:affiliation></affiliation></author><author><name sortKey="Alema, N" sort="Alema, N" uniqKey="Alema N" first="N." last="Alema">N. Alema</name><affiliation><mods:affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:356E1F0514CB08A534C75719FD6D48864D6DABDA</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1111/j.1439-0264.2005.00669_4.x</idno><idno type="url">https://api.istex.fr/document/356E1F0514CB08A534C75719FD6D48864D6DABDA/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001677</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001677</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title><author><name sortKey="Alexandre Pires, A" sort="Alexandre Pires, A" uniqKey="Alexandre Pires A" first="A." last="Alexandre- Pires">A. Alexandre- Pires</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Ferreira Ias, G" sort="Ferreira Ias, G" uniqKey="Ferreira Ias G" first="G." last="Ferreira-Dias">G. Ferreira-Dias</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Mateus, L" sort="Mateus, L" uniqKey="Mateus L" first="L." last="Mateus">L. Mateus</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Mendes Jorge, L" sort="Mendes Jorge, L" uniqKey="Mendes Jorge L" first="L." last="Mendes- Jorge">L. Mendes- Jorge</name><affiliation><mods:affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Cifuentes, J M" sort="Cifuentes, J M" uniqKey="Cifuentes J" first="J. M." last="Cifuentes">J. M. Cifuentes</name><affiliation><mods:affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</mods:affiliation></affiliation></author><author><name sortKey="Alema, N" sort="Alema, N" uniqKey="Alema N" first="N." last="Alema">N. Alema</name><affiliation><mods:affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Anatomia, Histologia, Embryologia</title><idno type="ISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12">2005-12</date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="2">2</biblScope><biblScope unit="page" to="2">2</biblScope></imprint><idno type="ISSN">0340-2096</idno></series><idno type="istex">356E1F0514CB08A534C75719FD6D48864D6DABDA</idno><idno type="DOI">10.1111/j.1439-0264.2005.00669_4.x</idno><idno type="ArticleID">AHE669_4_4</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0340-2096</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti‐angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected post mortem from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis (‘Scion Image, NIH, USA’) based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one‐way ANOVA and post‐comparison tests. There was a significant increase in microvascular areas in November (P < 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February (P < 0.05) and November (P < 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non‐breeding season in the male cat.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>A. Alexandre‐ Pires</name><affiliations><json:string>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>G. Ferreira‐Dias</name><affiliations><json:string>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>L. Mateus</name><affiliations><json:string>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>L. Mendes‐ Jorge</name><affiliations><json:string>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>J. M. Cifuentes</name><affiliations><json:string>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</json:string></affiliations></json:item><json:item><name>N. Alemañ</name><affiliations><json:string>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</json:string></affiliations></json:item></author><articleId><json:string>AHE669_4_4</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>abstract</json:string></originalGenre><abstract>Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti‐angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected post mortem from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis (‘Scion Image, NIH, USA’) based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one‐way ANOVA and post‐comparison tests. There was a significant increase in microvascular areas in November (P > 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February (P > 0.05) and November (P > 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non‐breeding season in the male cat.</abstract><qualityIndicators><score>8.5</score><pdfVersion>1.4</pdfVersion><pdfPageSize>595 x 842 pts (A4)</pdfPageSize><refBibsNative>false</refBibsNative><abstractCharCount>1905</abstractCharCount><pdfWordCount>39539</pdfWordCount><pdfCharCount>256516</pdfCharCount><pdfPageCount>59</pdfPageCount><abstractWordCount>281</abstractWordCount></qualityIndicators><title>Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title><genre><json:string>abstract</json:string></genre><host><volume>34</volume><publisherId><json:string>AHE</json:string></publisherId><pages><last>2</last><first>2</first></pages><issn><json:string>0340-2096</json:string></issn><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1439-0264</json:string></eissn><title>Anatomia, Histologia, Embryologia</title><doi><json:string>10.1111/(ISSN)1439-0264</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>veterinary sciences</json:string><json:string>anatomy & morphology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>developmental biology</json:string></scienceMetrix></categories><publicationDate>2005</publicationDate><copyrightDate>2005</copyrightDate><doi><json:string>10.1111/j.1439-0264.2005.00669_4.x</json:string></doi><id>356E1F0514CB08A534C75719FD6D48864D6DABDA</id><score>0.024552964</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/356E1F0514CB08A534C75719FD6D48864D6DABDA/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/356E1F0514CB08A534C75719FD6D48864D6DABDA/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/356E1F0514CB08A534C75719FD6D48864D6DABDA/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><availability><p>WILEY</p></availability><date>2005</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title><author xml:id="author-1"><persName><forename type="first">A.</forename><surname>Alexandre‐ Pires</surname></persName><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation></author><author xml:id="author-2"><persName><forename type="first">G.</forename><surname>Ferreira‐Dias</surname></persName><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation></author><author xml:id="author-3"><persName><forename type="first">L.</forename><surname>Mateus</surname></persName><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation></author><author xml:id="author-4"><persName><forename type="first">L.</forename><surname>Mendes‐ Jorge</surname></persName><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation></author><author xml:id="author-5"><persName><forename type="first">J. M.</forename><surname>Cifuentes</surname></persName><affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</affiliation></author><author xml:id="author-6"><persName><forename type="first">N.</forename><surname>Alemañ</surname></persName><affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</affiliation></author></analytic><monogr><title level="j">Anatomia, Histologia, Embryologia</title><idno type="pISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><idno type="DOI">10.1111/(ISSN)1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12"></date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="2">2</biblScope><biblScope unit="page" to="2">2</biblScope></imprint></monogr><idno type="istex">356E1F0514CB08A534C75719FD6D48864D6DABDA</idno><idno type="DOI">10.1111/j.1439-0264.2005.00669_4.x</idno><idno type="ArticleID">AHE669_4_4</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti‐angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected post mortem from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis (‘Scion Image, NIH, USA’) based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one‐way ANOVA and post‐comparison tests. There was a significant increase in microvascular areas in November (P < 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February (P < 0.05) and November (P < 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non‐breeding season in the male cat.</p></abstract></profileDesc><revisionDesc><change when="2005-12">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-02-9">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/356E1F0514CB08A534C75719FD6D48864D6DABDA/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley component found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Verlag GmbH</publisherName><publisherLoc>Berlin, Germany</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0264</doi><issn type="print">0340-2096</issn><issn type="electronic">1439-0264</issn><idGroup><id type="product" value="AHE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ANATOMIA HISTOLOGIA EMBRYOLOGIA">Anatomia, Histologia, Embryologia</title></titleGroup></publicationMeta><publicationMeta level="part" position="12000"><doi origin="wiley">10.1111/ahe.2005.34.issue-s1</doi><numberingGroup><numbering type="journalVolume" number="34">34</numbering><numbering type="supplement">s1</numbering></numberingGroup><coverDate startDate="2005-12">December 2005</coverDate></publicationMeta><publicationMeta level="unit" type="abstract" position="1" status="forIssue"><doi origin="wiley">10.1111/j.1439-0264.2005.00669_4.x</doi><idGroup><id type="unit" value="AHE669_4_4"></id></idGroup><titleGroup><title type="tocHeading1">Abstracts of the XXVth Congress of the European Association of Veterinary Anatomists (EAVA), Oslo, 28–31 July 2004</title></titleGroup><eventGroup><event type="firstOnline" date="2005-11-22"></event><event type="publishedOnlineFinalForm" date="2005-11-22"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:Header result:Header" date="2010-04-14"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="2">2</numbering><numbering type="pageLast" number="2">2</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:AHE.AHE669_4_4.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (<i>Felis catus</i>) Testes: Preliminary Results</title><title type="short">Abstracts</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a4-1"><personName><givenNames>A.</givenNames><familyName>Alexandre‐ Pires</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a4-1"><personName><givenNames>G.</givenNames><familyName>Ferreira‐Dias</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a4-1"><personName><givenNames>L.</givenNames><familyName>Mateus</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a4-1"><personName><givenNames>L.</givenNames><familyName>Mendes‐ Jorge</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a4-2"><personName><givenNames>J. M.</givenNames><familyName>Cifuentes</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a4-2"><personName><givenNames>N.</givenNames><familyName>Alemañ</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a4-1" countryCode="PT"><unparsedAffiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</unparsedAffiliation></affiliation><affiliation xml:id="a4-2" countryCode="ES"><unparsedAffiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti‐angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected <i>post mortem</i> from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis (‘Scion Image, NIH, USA’) based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one‐way ANOVA and post‐comparison tests. There was a significant increase in microvascular areas in November (<i>P </i>< 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February (<i>P </i>< 0.05) and November (<i>P </i>< 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non‐breeding season in the male cat.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Abstracts</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Alexandre‐ Pires</namePart><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Ferreira‐Dias</namePart><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Mateus</namePart><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Mendes‐ Jorge</namePart><affiliation>CIISA, Faculdade de Medicina Veterinária, Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Cifuentes</namePart><affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Alemañ</namePart><affiliation>Dpto. Anatomía, Facultad de Veterinária, U S C, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Verlag GmbH</publisher><place><placeTerm type="text">Berlin, Germany</placeTerm></place><dateIssued encoding="w3cdtf">2005-12</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti‐angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected post mortem from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis (‘Scion Image, NIH, USA’) based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one‐way ANOVA and post‐comparison tests. There was a significant increase in microvascular areas in November (P < 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February (P < 0.05) and November (P < 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non‐breeding season in the male cat.</abstract><relatedItem type="host"><titleInfo><title>Anatomia, Histologia, Embryologia</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0340-2096</identifier><identifier type="eISSN">1439-0264</identifier><identifier type="DOI">10.1111/(ISSN)1439-0264</identifier><identifier type="PublisherID">AHE</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>2</start><end>2</end></extent></part></relatedItem><identifier type="istex">356E1F0514CB08A534C75719FD6D48864D6DABDA</identifier><identifier type="DOI">10.1111/j.1439-0264.2005.00669_4.x</identifier><identifier type="ArticleID">AHE669_4_4</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Verlag GmbH</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001677 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001677 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:356E1F0514CB08A534C75719FD6D48864D6DABDA
|texte= Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results
}}
| This area was generated with Dilib version V0.6.27. |  |