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Occurrence and Distribution of Lactate Dehydrogenase in Knee Joint Carilage in Healthy and Osteoarthrotic Dogs

Identifieur interne : 001675 ( Istex/Corpus ); précédent : 001674; suivant : 001676

Occurrence and Distribution of Lactate Dehydrogenase in Knee Joint Carilage in Healthy and Osteoarthrotic Dogs

Auteurs : E. Walter ; D. Spreng ; A. E. Friess ; M. H. Stoffel

Source :

RBID : ISTEX:1F2C0040DD3AA43FF1D40C895074DB4634E4C111

Abstract

According to clinical studies, degenerative diseases of canine joints lead to higher lactate dehydrogenase (LDH) levels in synovial fluid. The goal of the present study was to examine the intraarticular distribution of LDH in healthy and osteoarthrotic knee joints in order to identify possible sources of LDH in synovial fluid. As synovial LDH concentrations neither correlate with the number of leukocytes nor with synovitis, our investigation focused on the articular cartilage. Samples from healthy and osteoarthrotic knee joints were fixed and processed for transmission electron microscopy (TEM), immunohistochemistry (IHC), and immunocytochemistry (ICC). In addition, fresh cartilage samples were investigated cytochemically by the tetrazolium‐formazan reaction. Analyses of blood and synovial fluid samples were used to confirm the absence of inflammatory disease. Morphology of articular cartilage was assessed macroscopically and by means of TEM. IHC revealed highest levels of LDH in chondrones and a diffuse labelling of the matrix with a distinctive decrease in signal from superficial to deeper cartilage layers. Ultrastructural localization by ICC showed LDH to be present in the cytoplasm of all chondrocytes and confirmed the density gradient in the matrix. Labelling was absent from nuclei and from pericellular rims. Cytochemistry confirmed the distribution pattern and, thus, expanded our findings beyond immunological evidence by providing proof of enzymatic activity of LDH in articular cartilage. The present results indicate that LDH is transferred from chondrocytes to the cartilaginous matrix. We suggest, therefore, that LDH found in synovial fluid originates from the articular cartilage and that osteoarthrotic processes promote LDH release from the cartilaginous matrix.

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DOI: 10.1111/j.1439-0264.2005.00669_127.x

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ISTEX:1F2C0040DD3AA43FF1D40C895074DB4634E4C111

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<affiliation>Department of Clinical Veterinary Medicine, Division of small animal surgery, University of Berne Veterinary School, Berne, Switzerland</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">A. E.</namePart>
<namePart type="family">Friess</namePart>
<affiliation>Institute of Veterinary Anatomy</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">M. H.</namePart>
<namePart type="family">Stoffel</namePart>
<affiliation>Institute of Veterinary Anatomy</affiliation>
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<roleTerm type="text">author</roleTerm>
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<typeOfResource>text</typeOfResource>
<genre type="abstract" displayLabel="abstract"></genre>
<originInfo>
<publisher>Blackwell Verlag GmbH</publisher>
<place>
<placeTerm type="text">Berlin, Germany</placeTerm>
</place>
<dateIssued encoding="w3cdtf">2005-12</dateIssued>
<copyrightDate encoding="w3cdtf">2005</copyrightDate>
</originInfo>
<language>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract lang="en">According to clinical studies, degenerative diseases of canine joints lead to higher lactate dehydrogenase (LDH) levels in synovial fluid. The goal of the present study was to examine the intraarticular distribution of LDH in healthy and osteoarthrotic knee joints in order to identify possible sources of LDH in synovial fluid. As synovial LDH concentrations neither correlate with the number of leukocytes nor with synovitis, our investigation focused on the articular cartilage. Samples from healthy and osteoarthrotic knee joints were fixed and processed for transmission electron microscopy (TEM), immunohistochemistry (IHC), and immunocytochemistry (ICC). In addition, fresh cartilage samples were investigated cytochemically by the tetrazolium‐formazan reaction. Analyses of blood and synovial fluid samples were used to confirm the absence of inflammatory disease. Morphology of articular cartilage was assessed macroscopically and by means of TEM. IHC revealed highest levels of LDH in chondrones and a diffuse labelling of the matrix with a distinctive decrease in signal from superficial to deeper cartilage layers. Ultrastructural localization by ICC showed LDH to be present in the cytoplasm of all chondrocytes and confirmed the density gradient in the matrix. Labelling was absent from nuclei and from pericellular rims. Cytochemistry confirmed the distribution pattern and, thus, expanded our findings beyond immunological evidence by providing proof of enzymatic activity of LDH in articular cartilage. The present results indicate that LDH is transferred from chondrocytes to the cartilaginous matrix. We suggest, therefore, that LDH found in synovial fluid originates from the articular cartilage and that osteoarthrotic processes promote LDH release from the cartilaginous matrix.</abstract>
<relatedItem type="host">
<titleInfo>
<title>Anatomia, Histologia, Embryologia</title>
</titleInfo>
<genre type="journal">journal</genre>
<identifier type="ISSN">0340-2096</identifier>
<identifier type="eISSN">1439-0264</identifier>
<identifier type="DOI">10.1111/(ISSN)1439-0264</identifier>
<identifier type="PublisherID">AHE</identifier>
<part>
<date>2005</date>
<detail type="volume">
<caption>vol.</caption>
<number>34</number>
</detail>
<detail type="supplement">
<caption>Suppl. no.</caption>
<number>s1</number>
</detail>
<extent unit="pages">
<start>56</start>
<end>56</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">1F2C0040DD3AA43FF1D40C895074DB4634E4C111</identifier>
<identifier type="DOI">10.1111/j.1439-0264.2005.00669_127.x</identifier>
<identifier type="ArticleID">AHE669_127_127</identifier>
<recordInfo>
<recordContentSource>WILEY</recordContentSource>
<recordOrigin>Blackwell Verlag GmbH</recordOrigin>
</recordInfo>
</mods>
</metadata>
<serie></serie>
</istex>
</record>

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