Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study
Identifieur interne : 001645 ( Istex/Corpus ); précédent : 001644; suivant : 001646Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study
Auteurs : Verona Villar-Cervi O ; Ant N Barreiro-Iglesias ; Blanca Fernández-L Pez ; Sylvie Mazan ; María Celina Rodicio ; Ram N Anad NSource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2013-02-15.
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Abstract
Glutamate is the major excitatory neurotransmitter in vertebrates, and glutamatergic cells probably represent a majority of neurons in the brain. Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium‐sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium‐sized cells of some reticulospinal‐projecting groups were often glutamate‐immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal‐projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non‐glutamatergic cells or neurons that co‐release both GABA and glutamate. J. Comp. Neurol. 521:522–557, 2013. © 2012 Wiley Periodicals, Inc.
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DOI: 10.1002/cne.23189
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last="Villar-Cervi O">Verona Villar-Cervi O</name><affiliation><mods:affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Instituto de Neurociencias, Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain</mods:affiliation></affiliation></author><author><name sortKey="Barreiro Glesias, Ant N" sort="Barreiro Glesias, Ant N" uniqKey="Barreiro Glesias A" first="Ant N" last="Barreiro-Iglesias">Ant N Barreiro-Iglesias</name><affiliation><mods:affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Center for Neural Repair and Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 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Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</mods:affiliation></affiliation></author><author><name sortKey="Anad N, Ram N" sort="Anad N, Ram N" uniqKey="Anad N R" first="Ram N" last="Anad N">Ram N Anad N</name><affiliation><mods:affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Campus Sur, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. 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Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium‐sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium‐sized cells of some reticulospinal‐projecting groups were often glutamate‐immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal‐projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non‐glutamatergic cells or neurons that co‐release both GABA and glutamate. J. Comp. Neurol. 521:522–557, 2013. © 2012 Wiley Periodicals, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Verona Villar‐Cerviño</name><affiliations><json:string>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</json:string><json:string>Instituto de Neurociencias, Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain</json:string></affiliations></json:item><json:item><name>Antón Barreiro‐Iglesias</name><affiliations><json:string>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</json:string><json:string>Center for Neural Repair and Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140</json:string></affiliations></json:item><json:item><name>Blanca Fernández‐lópez</name><affiliations><json:string>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</json:string></affiliations></json:item><json:item><name>Sylvie Mazan</name><affiliations><json:string>Développement et Evolution des Vertébrés, UMR7150 CNRS and Université Paris 6, Station Biologique, 29680 Roscoff, France</json:string></affiliations></json:item><json:item><name>María Celina Rodicio</name><affiliations><json:string>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</json:string></affiliations></json:item><json:item><name>Ramón Anadón</name><affiliations><json:string>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</json:string><json:string>Departamento de Biología Celular y Ecología, Facultad de Biología, Campus Sur, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>glutamate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>vesicular glutamate transporter</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>in situ hybridization</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>immunocytochemistry</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>neurobiotin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>reticulospinal system</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>GABA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>lamprey</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Agnathans</value></json:item></subject><articleId><json:string>CNE23189</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Glutamate is the major excitatory neurotransmitter in vertebrates, and glutamatergic cells probably represent a majority of neurons in the brain. Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium‐sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium‐sized cells of some reticulospinal‐projecting groups were often glutamate‐immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal‐projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non‐glutamatergic cells or neurons that co‐release both GABA and glutamate. J. Comp. Neurol. 521:522–557, 2013. © 2012 Wiley Periodicals, Inc.</abstract><qualityIndicators><score>8.5</score><pdfVersion>1.4</pdfVersion><pdfPageSize>612 x 810 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>2057</abstractCharCount><pdfWordCount>16552</pdfWordCount><pdfCharCount>109823</pdfCharCount><pdfPageCount>36</pdfPageCount><abstractWordCount>262</abstractWordCount></qualityIndicators><title>Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study</title><genre><json:string>article</json:string></genre><host><volume>521</volume><publisherId><json:string>CNE</json:string></publisherId><pages><total>36</total><last>557</last><first>522</first></pages><issn><json:string>0021-9967</json:string></issn><issue>3</issue><subject><json:item><value>Research Article</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1096-9861</json:string></eissn><title>Journal of Comparative Neurology</title><doi><json:string>10.1002/(ISSN)1096-9861</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>zoology</json:string><json:string>neurosciences</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>neurology & neurosurgery</json:string></scienceMetrix></categories><publicationDate>2013</publicationDate><copyrightDate>2013</copyrightDate><doi><json:string>10.1002/cne.23189</json:string></doi><id>E871511E107D10A903C2CD1C60E85F0E86A1DEA2</id><score>0.013024874</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/E871511E107D10A903C2CD1C60E85F0E86A1DEA2/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/E871511E107D10A903C2CD1C60E85F0E86A1DEA2/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/E871511E107D10A903C2CD1C60E85F0E86A1DEA2/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 2012 Wiley Periodicals, Inc.</p></availability><date>2013</date></publicationStmt><notesStmt><note>Spanish Ministry of Science and Innovation - No. BFU2007‐61056 and BFU2010‐17174 (to M.C.R.);</note><note>Xunta de Galicia - No. INCITE09ENA200036ES (to R.A.);</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study</title><author xml:id="author-1"><persName><forename type="first">Verona</forename><surname>Villar‐Cerviño</surname></persName><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><affiliation>Instituto de Neurociencias, Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">Antón</forename><surname>Barreiro‐Iglesias</surname></persName><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><affiliation>Center for Neural Repair and Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140</affiliation></author><author xml:id="author-3"><persName><forename type="first">Blanca</forename><surname>Fernández‐lópez</surname></persName><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">Sylvie</forename><surname>Mazan</surname></persName><affiliation>Développement et Evolution des Vertébrés, UMR7150 CNRS and Université Paris 6, Station Biologique, 29680 Roscoff, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">María Celina</forename><surname>Rodicio</surname></persName><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation></author><author xml:id="author-6"><persName><forename type="first">Ramón</forename><surname>Anadón</surname></persName><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Campus Sur, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation></author></analytic><monogr><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="pISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><idno type="DOI">10.1002/(ISSN)1096-9861</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2013-02-15"></date><biblScope unit="volume">521</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="522">522</biblScope><biblScope unit="page" to="557">557</biblScope></imprint></monogr><idno type="istex">E871511E107D10A903C2CD1C60E85F0E86A1DEA2</idno><idno type="DOI">10.1002/cne.23189</idno><idno type="ArticleID">CNE23189</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2013</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Glutamate is the major excitatory neurotransmitter in vertebrates, and glutamatergic cells probably represent a majority of neurons in the brain. Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium‐sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium‐sized cells of some reticulospinal‐projecting groups were often glutamate‐immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal‐projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non‐glutamatergic cells or neurons that co‐release both GABA and glutamate. J. Comp. Neurol. 521:522–557, 2013. © 2012 Wiley Periodicals, Inc.</p></abstract><abstract xml:lang="en" style="graphical"><p>The present in situ hybridization study provides an anatomical basis for the localization of glutamatergic neurons in the lamprey brainstem. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry. The results indicate that glutamate immunohistochemistry did not reveal the entire set of glutamatergic populations demonstrated by expression of vesicular glutamate transporter (VGLUT) mRNA, and this applies to the largest reticulospinal neurons. Colocalization of glutamate and GABA was observed in some small neurons, suggesting co‐release of GABA and glutamate.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>glutamate</term></item><item><term>vesicular glutamate transporter</term></item><item><term>in situ hybridization</term></item><item><term>immunocytochemistry</term></item><item><term>neurobiotin</term></item><item><term>reticulospinal system</term></item><item><term>GABA</term></item><item><term>lamprey</term></item><item><term>Agnathans</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-01-09">Received</change><change when="2012-07-06">Registration</change><change when="2013-02-15">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/E871511E107D10A903C2CD1C60E85F0E86A1DEA2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9861</doi><issn type="print">0021-9967</issn><issn type="electronic">1096-9861</issn><idGroup><id type="product" value="CNE"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF COMPARATIVE NEUROLOGY">Journal of Comparative Neurology</title><title type="short">J. 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Neurol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="30"><doi origin="wiley" registered="yes">10.1002/cne.v521.3</doi><numberingGroup><numbering type="journalVolume" number="521">521</numbering><numbering type="journalIssue">3</numbering></numberingGroup><coverDate startDate="2013-02-15">15 February 2013</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="30" status="forIssue"><doi origin="wiley" registered="yes">10.1002/cne.23189</doi><idGroup><id type="unit" value="CNE23189"></id></idGroup><countGroup><count type="pageTotal" number="36"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2012 Wiley Periodicals, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="2012-01-09"></event><event type="manuscriptAccepted" date="2012-07-06"></event><event 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number="18"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="123"></count><count type="wordTotal" number="18968"></count></countGroup><titleGroup><title type="main" xml:lang="en">Glutamatergic neuronal populations in the brainstem of the sea lamprey, <i>Petromyzon marinus</i>: An in situ hybridization and immunocytochemical study</title><title type="short" xml:lang="en">Glutamatergic Neurons in the Lamprey Brainstem</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1 #af2"><personName><givenNames>Verona</givenNames><familyName>Villar‐Cerviño</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1 #af3"><personName><givenNames>Antón</givenNames><familyName>Barreiro‐Iglesias</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Blanca</givenNames><familyName>Fernández‐lópez</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Sylvie</givenNames><familyName>Mazan</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1"><personName><givenNames>María Celina</givenNames><familyName>Rodicio</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Ramón</givenNames><familyName>Anadón</familyName></personName><contactDetails><email>ramon.anadon@usc.es</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="ES" type="organization"><unparsedAffiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="ES" type="organization"><unparsedAffiliation>Instituto de Neurociencias, Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="US" type="organization"><unparsedAffiliation>Center for Neural Repair and Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="FR" type="organization"><unparsedAffiliation>Développement et Evolution des Vertébrés, UMR7150 CNRS and Université Paris 6, Station Biologique, 29680 Roscoff, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">glutamate</keyword><keyword xml:id="kwd2">vesicular glutamate transporter</keyword><keyword xml:id="kwd3">in situ hybridization</keyword><keyword xml:id="kwd4">immunocytochemistry</keyword><keyword xml:id="kwd5">neurobiotin</keyword><keyword xml:id="kwd6">reticulospinal system</keyword><keyword xml:id="kwd7">GABA</keyword><keyword xml:id="kwd8">lamprey</keyword><keyword xml:id="kwd9">Agnathans</keyword></keywordGroup><fundingInfo><fundingAgency>Spanish Ministry of Science and Innovation</fundingAgency><fundingNumber>BFU2007‐61056 and BFU2010‐17174 (to M.C.R.)</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Xunta de Galicia</fundingAgency><fundingNumber>INCITE09ENA200036ES (to R.A.)</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Glutamate is the major excitatory neurotransmitter in vertebrates, and glutamatergic cells probably represent a majority of neurons in the brain. Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium‐sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium‐sized cells of some reticulospinal‐projecting groups were often glutamate‐immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal‐projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non‐glutamatergic cells or neurons that co‐release both GABA and glutamate. J. Comp. Neurol. 521:522–557, 2013. © 2012 Wiley Periodicals, Inc.</p></abstract><abstract type="graphical" xml:lang="en"><p>The present <i>in situ</i> hybridization study provides an anatomical basis for the localization of glutamatergic neurons in the lamprey brainstem. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry. The results indicate that glutamate immunohistochemistry did not reveal the entire set of glutamatergic populations demonstrated by expression of vesicular glutamate transporter (VGLUT) mRNA, and this applies to the largest reticulospinal neurons. Colocalization of glutamate and GABA was observed in some small neurons, suggesting co‐release of GABA and glutamate.</p><p><blockFixed type="graphic"><mediaResourceGroup><mediaResource alt="image" eRights="yes" copyright="Wiley Periodicals, Inc." href="urn:x-wiley:00219967:media:CNE23189:gra001"></mediaResource></mediaResourceGroup></blockFixed></p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Glutamatergic Neurons in the Lamprey Brainstem</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study</title></titleInfo><name type="personal"><namePart type="given">Verona</namePart><namePart type="family">Villar‐Cerviño</namePart><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><affiliation>Instituto de Neurociencias, Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Antón</namePart><namePart type="family">Barreiro‐Iglesias</namePart><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><affiliation>Center for Neural Repair and Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Blanca</namePart><namePart type="family">Fernández‐lópez</namePart><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sylvie</namePart><namePart type="family">Mazan</namePart><affiliation>Développement et Evolution des Vertébrés, UMR7150 CNRS and Université Paris 6, Station Biologique, 29680 Roscoff, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">María Celina</namePart><namePart type="family">Rodicio</namePart><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ramón</namePart><namePart type="family">Anadón</namePart><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><affiliation>Departamento de Biología Celular y Ecología, Facultad de Biología, Campus Sur, Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2013-02-15</dateIssued><dateCaptured encoding="w3cdtf">2012-01-09</dateCaptured><dateValid encoding="w3cdtf">2012-07-06</dateValid><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">18</extent><extent unit="references">123</extent><extent unit="words">18968</extent></physicalDescription><abstract lang="en">Glutamate is the major excitatory neurotransmitter in vertebrates, and glutamatergic cells probably represent a majority of neurons in the brain. Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium‐sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium‐sized cells of some reticulospinal‐projecting groups were often glutamate‐immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal‐projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non‐glutamatergic cells or neurons that co‐release both GABA and glutamate. J. Comp. Neurol. 521:522–557, 2013. © 2012 Wiley Periodicals, Inc.</abstract><abstract type="graphical" lang="en">The present in situ hybridization study provides an anatomical basis for the localization of glutamatergic neurons in the lamprey brainstem. We also combined glutamate immunohistochemistry with neuronal tract‐tracing methods or γ‐aminobutyric acid (GABA) immunohistochemistry. The results indicate that glutamate immunohistochemistry did not reveal the entire set of glutamatergic populations demonstrated by expression of vesicular glutamate transporter (VGLUT) mRNA, and this applies to the largest reticulospinal neurons. Colocalization of glutamate and GABA was observed in some small neurons, suggesting co‐release of GABA and glutamate.</abstract><note type="funding">Spanish Ministry of Science and Innovation - No. BFU2007‐61056 and BFU2010‐17174 (to M.C.R.); </note><note type="funding">Xunta de Galicia - No. INCITE09ENA200036ES (to R.A.); </note><subject lang="en"><genre>keywords</genre><topic>glutamate</topic><topic>vesicular glutamate transporter</topic><topic>in situ hybridization</topic><topic>immunocytochemistry</topic><topic>neurobiotin</topic><topic>reticulospinal system</topic><topic>GABA</topic><topic>lamprey</topic><topic>Agnathans</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>2013</date><detail type="volume"><caption>vol.</caption><number>521</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>522</start><end>557</end><total>36</total></extent></part></relatedItem><identifier type="istex">E871511E107D10A903C2CD1C60E85F0E86A1DEA2</identifier><identifier type="DOI">10.1002/cne.23189</identifier><identifier type="ArticleID">CNE23189</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2012 Wiley Periodicals, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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