Specific Localization of the Gap Junctional Protein Connexin 32 in Equine Gastric Mucosa
Identifieur interne : 001603 ( Istex/Corpus ); précédent : 001602; suivant : 001604Specific Localization of the Gap Junctional Protein Connexin 32 in Equine Gastric Mucosa
Auteurs : C. Fink ; T. Hembes ; R. Brehm ; R. Weigel ; C. Heeb ; M. Tatar ; M. KressinSource :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
Gap junctional inter‐cellular communication (GJIC) is known to be important in the maintenance of tissue homeostasis. Evidence is accumulating that GJIC plays an important role in the gastric mucosal defence system and that the loss of GJIC is associated with gastric ulcer formation. The prevalence of gastric ulceration in foals and horses is high. However, there are no studies about the presence of gap junctions in gastric mucosa of horses. The purpose of this study was to investigate the distribution pattern of the gap junction protein 32 in normal gastric mucosa of horses. Immunocytochemical observations were made with light microscopy on cryosections of fresh frozen gastric mucosa from the fundic region, pyloric region, margo plicatus and pars nonglandularis. Immunohistochemical staining was performed by standard immunoperoxidase techniques using an anti‐connexin 32 polyclonal antibody. In Situ Hybridization, Western Blot Analysis and RT‐PCR were performed to confirm the presence of connexin 32. In normal horse gastric mucosa, connexin 32 was abundant in the surface epithelium and in a decreasing staining gradient extending down to the neck of the glands. Gastric surface mucous cells are formed in the neck of the glands and migrate along the foveola toward the mucosal surface. So, immature forms of surface mucous cells are found in the generative zone and in the deep part of the foveola, whereas well‐matured ones cover the upper part of the foveola and the luminal surface of the mucosa. Therefore, these findings are in line with other studies, which described larger and more numerous gap junctions in mature surface mucous cells than in immature cells. These results show that gap junctions develop during the maturation of surface mucous cells and suggest that GJIC between gastric surface mucous cells plays an important role in the regulation of cell differentiation and in tissue homeostasis. Further studies are required to investigate the distribution pattern of connexin 32 in the gastric mucosa of horses with gastric ulcer.
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DOI: 10.1111/j.1439-0264.2005.00669_33.x
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ISTEX:D7F2032DB7D544905B2E1F92C02F31E04807A651Le document en format XML
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Fink</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hembes, T" sort="Hembes, T" uniqKey="Hembes T" first="T." last="Hembes">T. Hembes</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Brehm, R" sort="Brehm, R" uniqKey="Brehm R" first="R." last="Brehm">R. Brehm</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Weigel, R" sort="Weigel, R" uniqKey="Weigel R" first="R." last="Weigel">R. Weigel</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Heeb, C" sort="Heeb, C" uniqKey="Heeb C" first="C." last="Heeb">C. Heeb</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Tatar, M" sort="Tatar, M" uniqKey="Tatar M" first="M." last="Tatar">M. Tatar</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Kressin, M" sort="Kressin, M" uniqKey="Kressin M" first="M." last="Kressin">M. Kressin</name><affiliation><mods:affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Anatomia, Histologia, Embryologia</title><idno type="ISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12">2005-12</date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="15">15</biblScope><biblScope unit="page" to="15">15</biblScope></imprint><idno type="ISSN">0340-2096</idno></series><idno type="istex">D7F2032DB7D544905B2E1F92C02F31E04807A651</idno><idno type="DOI">10.1111/j.1439-0264.2005.00669_33.x</idno><idno type="ArticleID">AHE669_33_33</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0340-2096</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Gap junctional inter‐cellular communication (GJIC) is known to be important in the maintenance of tissue homeostasis. Evidence is accumulating that GJIC plays an important role in the gastric mucosal defence system and that the loss of GJIC is associated with gastric ulcer formation. The prevalence of gastric ulceration in foals and horses is high. However, there are no studies about the presence of gap junctions in gastric mucosa of horses. The purpose of this study was to investigate the distribution pattern of the gap junction protein 32 in normal gastric mucosa of horses. Immunocytochemical observations were made with light microscopy on cryosections of fresh frozen gastric mucosa from the fundic region, pyloric region, margo plicatus and pars nonglandularis. Immunohistochemical staining was performed by standard immunoperoxidase techniques using an anti‐connexin 32 polyclonal antibody. In Situ Hybridization, Western Blot Analysis and RT‐PCR were performed to confirm the presence of connexin 32. In normal horse gastric mucosa, connexin 32 was abundant in the surface epithelium and in a decreasing staining gradient extending down to the neck of the glands. Gastric surface mucous cells are formed in the neck of the glands and migrate along the foveola toward the mucosal surface. So, immature forms of surface mucous cells are found in the generative zone and in the deep part of the foveola, whereas well‐matured ones cover the upper part of the foveola and the luminal surface of the mucosa. Therefore, these findings are in line with other studies, which described larger and more numerous gap junctions in mature surface mucous cells than in immature cells. These results show that gap junctions develop during the maturation of surface mucous cells and suggest that GJIC between gastric surface mucous cells plays an important role in the regulation of cell differentiation and in tissue homeostasis. Further studies are required to investigate the distribution pattern of connexin 32 in the gastric mucosa of horses with gastric ulcer.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>C. Fink</name><affiliations><json:string>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</json:string></affiliations></json:item><json:item><name>T. Hembes</name><affiliations><json:string>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</json:string></affiliations></json:item><json:item><name>R. Brehm</name><affiliations><json:string>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</json:string></affiliations></json:item><json:item><name>R. 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Kressin</name><affiliations><json:string>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</json:string></affiliations></json:item></author><articleId><json:string>AHE669_33_33</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>abstract</json:string></originalGenre><abstract>Gap junctional inter‐cellular communication (GJIC) is known to be important in the maintenance of tissue homeostasis. Evidence is accumulating that GJIC plays an important role in the gastric mucosal defence system and that the loss of GJIC is associated with gastric ulcer formation. The prevalence of gastric ulceration in foals and horses is high. However, there are no studies about the presence of gap junctions in gastric mucosa of horses. The purpose of this study was to investigate the distribution pattern of the gap junction protein 32 in normal gastric mucosa of horses. Immunocytochemical observations were made with light microscopy on cryosections of fresh frozen gastric mucosa from the fundic region, pyloric region, margo plicatus and pars nonglandularis. Immunohistochemical staining was performed by standard immunoperoxidase techniques using an anti‐connexin 32 polyclonal antibody. In Situ Hybridization, Western Blot Analysis and RT‐PCR were performed to confirm the presence of connexin 32. In normal horse gastric mucosa, connexin 32 was abundant in the surface epithelium and in a decreasing staining gradient extending down to the neck of the glands. Gastric surface mucous cells are formed in the neck of the glands and migrate along the foveola toward the mucosal surface. So, immature forms of surface mucous cells are found in the generative zone and in the deep part of the foveola, whereas well‐matured ones cover the upper part of the foveola and the luminal surface of the mucosa. Therefore, these findings are in line with other studies, which described larger and more numerous gap junctions in mature surface mucous cells than in immature cells. These results show that gap junctions develop during the maturation of surface mucous cells and suggest that GJIC between gastric surface mucous cells plays an important role in the regulation of cell differentiation and in tissue homeostasis. 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Evidence is accumulating that GJIC plays an important role in the gastric mucosal defence system and that the loss of GJIC is associated with gastric ulcer formation. The prevalence of gastric ulceration in foals and horses is high. However, there are no studies about the presence of gap junctions in gastric mucosa of horses. The purpose of this study was to investigate the distribution pattern of the gap junction protein 32 in normal gastric mucosa of horses. Immunocytochemical observations were made with light microscopy on cryosections of fresh frozen gastric mucosa from the fundic region, pyloric region, margo plicatus and pars nonglandularis. Immunohistochemical staining was performed by standard immunoperoxidase techniques using an anti‐connexin 32 polyclonal antibody. In Situ Hybridization, Western Blot Analysis and RT‐PCR were performed to confirm the presence of connexin 32. In normal horse gastric mucosa, connexin 32 was abundant in the surface epithelium and in a decreasing staining gradient extending down to the neck of the glands. Gastric surface mucous cells are formed in the neck of the glands and migrate along the foveola toward the mucosal surface. So, immature forms of surface mucous cells are found in the generative zone and in the deep part of the foveola, whereas well‐matured ones cover the upper part of the foveola and the luminal surface of the mucosa. Therefore, these findings are in line with other studies, which described larger and more numerous gap junctions in mature surface mucous cells than in immature cells. These results show that gap junctions develop during the maturation of surface mucous cells and suggest that GJIC between gastric surface mucous cells plays an important role in the regulation of cell differentiation and in tissue homeostasis. Further studies are required to investigate the distribution pattern of connexin 32 in the gastric mucosa of horses with gastric ulcer.</p></abstract></profileDesc><revisionDesc><change when="2005-12">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-02-8">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/D7F2032DB7D544905B2E1F92C02F31E04807A651/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley component found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Verlag GmbH</publisherName><publisherLoc>Berlin, Germany</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0264</doi><issn type="print">0340-2096</issn><issn type="electronic">1439-0264</issn><idGroup><id type="product" value="AHE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ANATOMIA HISTOLOGIA EMBRYOLOGIA">Anatomia, Histologia, Embryologia</title></titleGroup></publicationMeta><publicationMeta level="part" position="12000"><doi origin="wiley">10.1111/ahe.2005.34.issue-s1</doi><numberingGroup><numbering type="journalVolume" number="34">34</numbering><numbering type="supplement">s1</numbering></numberingGroup><coverDate startDate="2005-12">December 2005</coverDate></publicationMeta><publicationMeta level="unit" type="abstract" position="1" status="forIssue"><doi origin="wiley">10.1111/j.1439-0264.2005.00669_33.x</doi><idGroup><id type="unit" value="AHE669_33_33"></id></idGroup><titleGroup><title type="tocHeading1">Abstracts of the XXVth Congress of the European Association of Veterinary Anatomists (EAVA), Oslo, 28–31 July 2004</title></titleGroup><eventGroup><event type="firstOnline" date="2005-11-22"></event><event type="publishedOnlineFinalForm" date="2005-11-22"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:Header result:Header" date="2010-04-14"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="15">15</numbering><numbering type="pageLast" number="15">15</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:AHE.AHE669_33_33.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Specific Localization of the Gap Junctional Protein Connexin 32 in Equine Gastric Mucosa</title><title type="short">Abstracts</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1"><personName><givenNames>C.</givenNames><familyName>Fink</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>T.</givenNames><familyName>Hembes</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>R.</givenNames><familyName>Brehm</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#aff-1-1"><personName><givenNames>R.</givenNames><familyName>Weigel</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#aff-1-1"><personName><givenNames>C.</givenNames><familyName>Heeb</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#aff-1-1"><personName><givenNames>M.</givenNames><familyName>Tatar</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#aff-1-1"><personName><givenNames>M.</givenNames><familyName>Kressin</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="DE"><unparsedAffiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Gap junctional inter‐cellular communication (GJIC) is known to be important in the maintenance of tissue homeostasis. Evidence is accumulating that GJIC plays an important role in the gastric mucosal defence system and that the loss of GJIC is associated with gastric ulcer formation. The prevalence of gastric ulceration in foals and horses is high. However, there are no studies about the presence of gap junctions in gastric mucosa of horses. The purpose of this study was to investigate the distribution pattern of the gap junction protein 32 in normal gastric mucosa of horses. Immunocytochemical observations were made with light microscopy on cryosections of fresh frozen gastric mucosa from the fundic region, pyloric region, <i>margo plicatus</i> and <i>pars nonglandularis</i>. Immunohistochemical staining was performed by standard immunoperoxidase techniques using an anti‐connexin 32 polyclonal antibody. <i>In Situ</i> Hybridization, Western Blot Analysis and RT‐PCR were performed to confirm the presence of connexin 32. In normal horse gastric mucosa, connexin 32 was abundant in the surface epithelium and in a decreasing staining gradient extending down to the neck of the glands. Gastric surface mucous cells are formed in the neck of the glands and migrate along the foveola toward the mucosal surface. So, immature forms of surface mucous cells are found in the generative zone and in the deep part of the foveola, whereas well‐matured ones cover the upper part of the foveola and the luminal surface of the mucosa. Therefore, these findings are in line with other studies, which described larger and more numerous gap junctions in mature surface mucous cells than in immature cells. These results show that gap junctions develop during the maturation of surface mucous cells and suggest that GJIC between gastric surface mucous cells plays an important role in the regulation of cell differentiation and in tissue homeostasis. Further studies are required to investigate the distribution pattern of connexin 32 in the gastric mucosa of horses with gastric ulcer.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Specific Localization of the Gap Junctional Protein Connexin 32 in Equine Gastric Mucosa</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Abstracts</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Specific Localization of the Gap Junctional Protein Connexin 32 in Equine Gastric Mucosa</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Fink</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Hembes</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Brehm</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Weigel</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Heeb</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Tatar</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Kressin</namePart><affiliation>Institut für Veterinär‐Anatomie, Histologie und Embryologie der Justus‐Liebig‐Universität Giessen, Frankfurter Str.98, D‐35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Verlag GmbH</publisher><place><placeTerm type="text">Berlin, Germany</placeTerm></place><dateIssued encoding="w3cdtf">2005-12</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Gap junctional inter‐cellular communication (GJIC) is known to be important in the maintenance of tissue homeostasis. Evidence is accumulating that GJIC plays an important role in the gastric mucosal defence system and that the loss of GJIC is associated with gastric ulcer formation. The prevalence of gastric ulceration in foals and horses is high. However, there are no studies about the presence of gap junctions in gastric mucosa of horses. The purpose of this study was to investigate the distribution pattern of the gap junction protein 32 in normal gastric mucosa of horses. Immunocytochemical observations were made with light microscopy on cryosections of fresh frozen gastric mucosa from the fundic region, pyloric region, margo plicatus and pars nonglandularis. Immunohistochemical staining was performed by standard immunoperoxidase techniques using an anti‐connexin 32 polyclonal antibody. In Situ Hybridization, Western Blot Analysis and RT‐PCR were performed to confirm the presence of connexin 32. In normal horse gastric mucosa, connexin 32 was abundant in the surface epithelium and in a decreasing staining gradient extending down to the neck of the glands. Gastric surface mucous cells are formed in the neck of the glands and migrate along the foveola toward the mucosal surface. So, immature forms of surface mucous cells are found in the generative zone and in the deep part of the foveola, whereas well‐matured ones cover the upper part of the foveola and the luminal surface of the mucosa. Therefore, these findings are in line with other studies, which described larger and more numerous gap junctions in mature surface mucous cells than in immature cells. These results show that gap junctions develop during the maturation of surface mucous cells and suggest that GJIC between gastric surface mucous cells plays an important role in the regulation of cell differentiation and in tissue homeostasis. Further studies are required to investigate the distribution pattern of connexin 32 in the gastric mucosa of horses with gastric ulcer.</abstract><relatedItem type="host"><titleInfo><title>Anatomia, Histologia, Embryologia</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0340-2096</identifier><identifier type="eISSN">1439-0264</identifier><identifier type="DOI">10.1111/(ISSN)1439-0264</identifier><identifier type="PublisherID">AHE</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>15</start><end>15</end></extent></part></relatedItem><identifier type="istex">D7F2032DB7D544905B2E1F92C02F31E04807A651</identifier><identifier type="DOI">10.1111/j.1439-0264.2005.00669_33.x</identifier><identifier type="ArticleID">AHE669_33_33</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Verlag GmbH</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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