Revisiting the origin of the vertebrate Hox14 by including its relict sarcopterygian members
Identifieur interne : 001490 ( Istex/Corpus ); précédent : 001489; suivant : 001491Revisiting the origin of the vertebrate Hox14 by including its relict sarcopterygian members
Auteurs : Nathalie Feiner ; Rolf Ericsson ; Axel Meyer ; Shigehiro KurakuSource :
- Journal of Experimental Zoology Part B: Molecular and Developmental Evolution [ 1552-5007 ] ; 2011-11-15.
Abstract
Bilaterian Hox genes play pivotal roles in the specification of positional identities along the anteroposterior axis. Particularly in vertebrates, their regulation is tightly coordinated by tandem arrays of genes [paralogy groups (PGs)] in four gene clusters (HoxA‐D). Traditionally, the uninterrupted Hox cluster (Hox1‐14) of the invertebrate chordate amphioxus was regarded as an archetype of the vertebrate Hox clusters. In contrast to Hox1‐13 that are globally regulated by the “Hox code” and are often phylogenetically conserved, vertebrate Hox14 members were only recently revealed to be present in an African lungfish, a coelacanth, chondrichthyans and a lamprey, and decoupled from the Hox code. In this study we performed a PCR‐based search of Hox14 members from diverse vertebrates, and identified one in the Australian lungfish, Neoceratodus forsteri. Based on a molecular phylogenetic analysis, this gene was designated NfHoxA14. Our real‐time RT‐PCR suggested its hindgut‐associated expression, previously observed also in cloudy catshark HoxD14 and lamprey Hox14α. It is likely that this altered expression scheme was established before the Hox cluster quadruplication, probably at the base of extant vertebrates. To investigate the origin of vertebrate Hox14, by including this sarcopterygian Hox14 member, we performed focused phylogenetic analyses on its relationship with other vertebrate posterior Hox PGs (Hox9‐13) as well as amphioxus posterior Hox genes. Our results confirmed the hypotheses previously proposed by other studies that vertebrate Hox14 does not have any amphioxus ortholog, and that none of 1‐to‐1 pairs of vertebrate and amphioxus posterior Hox genes, based on their relative location in the clusters, is orthologous. J. Exp. Zool. (Mol. Dev. Evol.) 316:515–525, 2011. © 2011 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/jez.b.21426
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Particularly in vertebrates, their regulation is tightly coordinated by tandem arrays of genes [paralogy groups (PGs)] in four gene clusters (HoxA‐D). Traditionally, the uninterrupted Hox cluster (Hox1‐14) of the invertebrate chordate amphioxus was regarded as an archetype of the vertebrate Hox clusters. In contrast to Hox1‐13 that are globally regulated by the “Hox code” and are often phylogenetically conserved, vertebrate Hox14 members were only recently revealed to be present in an African lungfish, a coelacanth, chondrichthyans and a lamprey, and decoupled from the Hox code. In this study we performed a PCR‐based search of Hox14 members from diverse vertebrates, and identified one in the Australian lungfish, Neoceratodus forsteri. Based on a molecular phylogenetic analysis, this gene was designated NfHoxA14. Our real‐time RT‐PCR suggested its hindgut‐associated expression, previously observed also in cloudy catshark HoxD14 and lamprey Hox14α. It is likely that this altered expression scheme was established before the Hox cluster quadruplication, probably at the base of extant vertebrates. To investigate the origin of vertebrate Hox14, by including this sarcopterygian Hox14 member, we performed focused phylogenetic analyses on its relationship with other vertebrate posterior Hox PGs (Hox9‐13) as well as amphioxus posterior Hox genes. Our results confirmed the hypotheses previously proposed by other studies that vertebrate Hox14 does not have any amphioxus ortholog, and that none of 1‐to‐1 pairs of vertebrate and amphioxus posterior Hox genes, based on their relative location in the clusters, is orthologous. J. Exp. Zool. (Mol. Dev. 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Particularly in vertebrates, their regulation is tightly coordinated by tandem arrays of genes [paralogy groups (PGs)] in four gene clusters (HoxA‐D). Traditionally, the uninterrupted Hox cluster (Hox1‐14) of the invertebrate chordate amphioxus was regarded as an archetype of the vertebrate Hox clusters. In contrast to Hox1‐13 that are globally regulated by the “Hox code” and are often phylogenetically conserved, vertebrate Hox14 members were only recently revealed to be present in an African lungfish, a coelacanth, chondrichthyans and a lamprey, and decoupled from the Hox code. In this study we performed a PCR‐based search of Hox14 members from diverse vertebrates, and identified one in the Australian lungfish, Neoceratodus forsteri. Based on a molecular phylogenetic analysis, this gene was designated NfHoxA14. Our real‐time RT‐PCR suggested its hindgut‐associated expression, previously observed also in cloudy catshark HoxD14 and lamprey Hox14α. It is likely that this altered expression scheme was established before the Hox cluster quadruplication, probably at the base of extant vertebrates. To investigate the origin of vertebrate Hox14, by including this sarcopterygian Hox14 member, we performed focused phylogenetic analyses on its relationship with other vertebrate posterior Hox PGs (Hox9‐13) as well as amphioxus posterior Hox genes. Our results confirmed the hypotheses previously proposed by other studies that vertebrate Hox14 does not have any amphioxus ortholog, and that none of 1‐to‐1 pairs of vertebrate and amphioxus posterior Hox genes, based on their relative location in the clusters, is orthologous. J. Exp. Zool. (Mol. Dev. 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Particularly in vertebrates, their regulation is tightly coordinated by tandem arrays of genes [paralogy groups (PGs)] in four gene clusters (HoxA‐D). Traditionally, the uninterrupted Hox cluster (Hox1‐14) of the invertebrate chordate amphioxus was regarded as an archetype of the vertebrate Hox clusters. In contrast to Hox1‐13 that are globally regulated by the “Hox code” and are often phylogenetically conserved, vertebrate Hox14 members were only recently revealed to be present in an African lungfish, a coelacanth, chondrichthyans and a lamprey, and decoupled from the Hox code. In this study we performed a PCR‐based search of Hox14 members from diverse vertebrates, and identified one in the Australian lungfish, Neoceratodus forsteri. Based on a molecular phylogenetic analysis, this gene was designated NfHoxA14. Our real‐time RT‐PCR suggested its hindgut‐associated expression, previously observed also in cloudy catshark HoxD14 and lamprey Hox14α. It is likely that this altered expression scheme was established before the Hox cluster quadruplication, probably at the base of extant vertebrates. To investigate the origin of vertebrate Hox14, by including this sarcopterygian Hox14 member, we performed focused phylogenetic analyses on its relationship with other vertebrate posterior Hox PGs (Hox9‐13) as well as amphioxus posterior Hox genes. Our results confirmed the hypotheses previously proposed by other studies that vertebrate Hox14 does not have any amphioxus ortholog, and that none of 1‐to‐1 pairs of vertebrate and amphioxus posterior Hox genes, based on their relative location in the clusters, is orthologous. J. Exp. Zool. (Mol. Dev. 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currentRef="#curr1"><personName><givenNames>Rolf</givenNames><familyName>Ericsson</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1 #af2 #af4"><personName><givenNames>Axel</givenNames><familyName>Meyer</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1 #af2 #af4" corresponding="yes"><personName><givenNames>Shigehiro</givenNames><familyName>Kuraku</familyName></personName><contactDetails><email normalForm="shigehiro.kuraku@uni-konstanz.de">shigehiro.kuraku@uni‐konstanz.de</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="DE" type="organization"><unparsedAffiliation>Department of Biology, University of Konstanz, Konstanz, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="DE" type="organization"><unparsedAffiliation>International Max‐Planck Research School (IMPRS) for Organismal Biology, University of Konstanz, Konstanz, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="AU" type="organization"><unparsedAffiliation>Biological Sciences, Macquarie University, Sydney, New South Wales, Australia</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="DE" type="organization"><unparsedAffiliation>Konstanz Research School Chemical Biology (KoRS‐CB), University of Konstanz, Konstanz, Germany</unparsedAffiliation></affiliation><affiliation xml:id="curr1" countryCode="GB"><unparsedAffiliation>The Natural History Museum, Palaeontology Department, Cromwell Road, London SW7 BD, United Kingdom</unparsedAffiliation></affiliation></affiliationGroup><fundingInfo><fundingAgency>University of Konstanz, International Max Planck Research School (IMPRS) for Organismal Biology</fundingAgency></fundingInfo><fundingInfo><fundingAgency>German Research Foundation</fundingAgency><fundingNumber>KU2669/1‐1</fundingNumber></fundingInfo><supportingInformation><p> Additional Supporting information may be found in the online version of this article </p><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:15525007:media:jez21426:jez_21426_sm_SuppInfo"></mediaResource><caption>Supplementary Materials</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Bilaterian <i>Hox</i> genes play pivotal roles in the specification of positional identities along the anteroposterior axis. Particularly in vertebrates, their regulation is tightly coordinated by tandem arrays of genes [paralogy groups (PGs)] in four gene clusters (HoxA‐D). Traditionally, the uninterrupted <i>Hox</i> cluster (<i>Hox1‐14</i>) of the invertebrate chordate amphioxus was regarded as an archetype of the vertebrate <i>Hox</i> clusters. In contrast to Hox1‐13 that are globally regulated by the “Hox code” and are often phylogenetically conserved, vertebrate Hox14 members were only recently revealed to be present in an African lungfish, a coelacanth, chondrichthyans and a lamprey, and decoupled from the Hox code. In this study we performed a PCR‐based search of Hox14 members from diverse vertebrates, and identified one in the Australian lungfish, <i>Neoceratodus forsteri</i>. Based on a molecular phylogenetic analysis, this gene was designated <i>NfHoxA14</i>. Our real‐time RT‐PCR suggested its hindgut‐associated expression, previously observed also in cloudy catshark <i>HoxD14</i> and lamprey <i>Hox14</i>α. It is likely that this altered expression scheme was established before the <i>Hox</i> cluster quadruplication, probably at the base of extant vertebrates. To investigate the origin of vertebrate Hox14, by including this sarcopterygian Hox14 member, we performed focused phylogenetic analyses on its relationship with other vertebrate posterior Hox PGs (Hox9‐13) as well as amphioxus posterior <i>Hox</i> genes. Our results confirmed the hypotheses previously proposed by other studies that vertebrate Hox14 does not have any amphioxus ortholog, and that none of 1‐to‐1 pairs of vertebrate and amphioxus posterior <i>Hox</i> genes, based on their relative location in the clusters, is orthologous. <i>J. Exp. Zool. (Mol. Dev. Evol.) 316:515–525, 2011</i>. © 2011 Wiley Periodicals, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Revisiting the origin of the vertebrate Hox14 by including its relict sarcopterygian members</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>PHYLOGENY OF VERTEBRATE Hox14</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Revisiting the origin of the vertebrate Hox14 by including its relict sarcopterygian members</title></titleInfo><name type="personal"><namePart type="given">Nathalie</namePart><namePart type="family">Feiner</namePart><affiliation>Department of Biology, University of Konstanz, Konstanz, Germany</affiliation><affiliation>International Max‐Planck Research School (IMPRS) for Organismal Biology, University of Konstanz, Konstanz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rolf</namePart><namePart type="family">Ericsson</namePart><affiliation>Biological Sciences, Macquarie University, Sydney, New South Wales, Australia</affiliation><affiliation>Current Address: The Natural History Museum, Palaeontology Department, Cromwell Road, London SW7 BD, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Axel</namePart><namePart type="family">Meyer</namePart><affiliation>Department of Biology, University of Konstanz, Konstanz, Germany</affiliation><affiliation>International Max‐Planck Research School (IMPRS) for Organismal Biology, University of Konstanz, Konstanz, Germany</affiliation><affiliation>Konstanz Research School Chemical Biology (KoRS‐CB), University of Konstanz, Konstanz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shigehiro</namePart><namePart type="family">Kuraku</namePart><affiliation>Department of Biology, University of Konstanz, Konstanz, Germany</affiliation><affiliation>International Max‐Planck Research School (IMPRS) for Organismal Biology, University of Konstanz, Konstanz, Germany</affiliation><affiliation>Konstanz Research School Chemical Biology (KoRS‐CB), University of Konstanz, Konstanz, Germany</affiliation><affiliation>Department of Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2011-11-15</dateIssued><dateCaptured encoding="w3cdtf">2011-04-01</dateCaptured><dateValid encoding="w3cdtf">2011-06-18</dateValid><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="references">55</extent></physicalDescription><abstract lang="en">Bilaterian Hox genes play pivotal roles in the specification of positional identities along the anteroposterior axis. Particularly in vertebrates, their regulation is tightly coordinated by tandem arrays of genes [paralogy groups (PGs)] in four gene clusters (HoxA‐D). Traditionally, the uninterrupted Hox cluster (Hox1‐14) of the invertebrate chordate amphioxus was regarded as an archetype of the vertebrate Hox clusters. In contrast to Hox1‐13 that are globally regulated by the “Hox code” and are often phylogenetically conserved, vertebrate Hox14 members were only recently revealed to be present in an African lungfish, a coelacanth, chondrichthyans and a lamprey, and decoupled from the Hox code. In this study we performed a PCR‐based search of Hox14 members from diverse vertebrates, and identified one in the Australian lungfish, Neoceratodus forsteri. Based on a molecular phylogenetic analysis, this gene was designated NfHoxA14. Our real‐time RT‐PCR suggested its hindgut‐associated expression, previously observed also in cloudy catshark HoxD14 and lamprey Hox14α. It is likely that this altered expression scheme was established before the Hox cluster quadruplication, probably at the base of extant vertebrates. To investigate the origin of vertebrate Hox14, by including this sarcopterygian Hox14 member, we performed focused phylogenetic analyses on its relationship with other vertebrate posterior Hox PGs (Hox9‐13) as well as amphioxus posterior Hox genes. Our results confirmed the hypotheses previously proposed by other studies that vertebrate Hox14 does not have any amphioxus ortholog, and that none of 1‐to‐1 pairs of vertebrate and amphioxus posterior Hox genes, based on their relative location in the clusters, is orthologous. J. Exp. Zool. (Mol. Dev. Evol.) 316:515–525, 2011. © 2011 Wiley Periodicals, Inc.</abstract><note type="funding">University of Konstanz, International Max Planck Research School (IMPRS) for Organismal Biology</note><note type="funding">German Research Foundation - No. KU2669/1‐1; </note><relatedItem type="host"><titleInfo><title>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</title></titleInfo><titleInfo type="abbreviated"><title>J. Exp. Zool.</title></titleInfo><genre type="journal">journal</genre><note type="content"> Additional Supporting information may be found in the online version of this articleSupporting Info Item: Supplementary Materials - </note><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">1552-5007</identifier><identifier type="eISSN">1552-5015</identifier><identifier type="DOI">10.1002/(ISSN)1552-5015</identifier><identifier type="PublisherID">JEZ</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>316B</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>515</start><end>525</end><total>11</total></extent></part></relatedItem><relatedItem type="preceding"><titleInfo><title>Journal of Experimental Zoology</title></titleInfo><identifier type="ISSN">0022-104X</identifier><identifier type="ISSN">1097-010X</identifier><part><date point="end">2004</date></part></relatedItem><identifier type="istex">C4C11F030385E9DCE11EA150773B9AC97E0A9694</identifier><identifier type="DOI">10.1002/jez.b.21426</identifier><identifier type="ArticleID">JEZ21426</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Wiley Periodicals, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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